| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
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| Outline of Final Research Achievements |
This work aims to investigate biosilicification mechanism in geothermal environment. To complete the study, we carried out metagenomic analysis of microflora in geothermal water and silica scale formed in the geothermal water. Comparative genome analysis showed specific hyperthermophilc microrganisms habitat in only silica scale. According to the results, we examined biosilicification abilities of T. thermophilus, G. kaustophilus, and P. calidifontis as representatives. Among these thermophiles, availability of silica biodeposition of T. thermophilus was significant. We conclude that cell surface proteins of T. thermophilus (molecular weight; 45、57、70 kDa) function to interact with supersaturated silica in environment. These proteins are annotated periplasm localized ABC transporters by ESI-MS/MS analysis.
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