Investigation of biosilicification mechanism in geothermal environment
| Project/Area Number |
26292182
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental agriculture(including landscape science)
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| Research Institution | Kyushu University |
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Principal Investigator |
Doi Katsumi 九州大学, 農学研究院, 教授 (40253520)
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| Co-Investigator(Kenkyū-buntansha) |
田代 康介 九州大学, 農学研究院, 准教授 (00192170)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
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| Keywords | シリカ / バイオミネラリゼーション / メタゲノム / Thermus / Geobacillus / 鉄飢餓 / プロモーター / シリカスケール / シリカ誘導性タンパク質 / γ-Proteobacteria門 / プロモーターアッセイ / Thermus thermopiles / 金属元素 / Thermus thermopilus / 超好熱アーキア / Feric uptake regulator / 環境 / 応用微生物 / ゲノム / シグナル伝達 / 金属応答 / 好熱微生物 |
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| Outline of Final Research Achievements |
This work aims to investigate biosilicification mechanism in geothermal environment. To complete the study, we carried out metagenomic analysis of microflora in geothermal water and silica scale formed in the geothermal water. Comparative genome analysis showed specific hyperthermophilc microrganisms habitat in only silica scale. According to the results, we examined biosilicification abilities of T. thermophilus, G. kaustophilus, and P. calidifontis as representatives. Among these thermophiles, availability of silica biodeposition of T. thermophilus was significant. We conclude that cell surface proteins of T. thermophilus (molecular weight; 45、57、70 kDa) function to interact with supersaturated silica in environment. These proteins are annotated periplasm localized ABC transporters by ESI-MS/MS analysis.
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Report
(5 results)
Research Products
(48 results)
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[Journal Article] Physiological Properties and Genome Structure of the Hyperthermophilic Filamentous Phage φOH3 Which Infects Thermus thermophilus HB8.2016
Author(s)
Nagayoshi Y, Kumagae K, Mori K, Tashiro K, Nakamura A, Fujino Y, Hiromasa Y, Iwamoto T, Kuhara S, Ohshima T, Doi K.
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Journal Title
Front Microbiol.
Volume: -
Pages: 1-11
DOI
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Peer Reviewed / Open Access
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[Journal Article] Wild mushrooms in Nepal : some potential candidates as antioxidant and ACE-inhibition sources.2014
Author(s)
Hai Bang T, Suhara H, Doi K, Ishikawa H, Fukami K, Parajuli GP, Katakura Y, Yamashita S. Watanabe K, Adhikari MK, Manandhar HK, Kondo R, Shimizu K.
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Journal Title
Evidence-based Complementary and Alternative Medicine
Volume: 2014
Issue: 1
Pages: 195305-195305
DOI
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Peer Reviewed / Open Access
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[Presentation] 好熱性細菌によるシリカ沈殿機構の解明2016
Author(s)
岡田 智紗, 藤野 泰寛, 花島 映里, 横山 拓史, 上田 晃, 土居 克実
Organizer
日本農芸化学会2016年度大会
Place of Presentation
札幌コンベンションセンター
Year and Date
2016-03-29
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[Book] アーキア生物学2017
Author(s)
永吉 佑子, 土居 克実
Total Pages
212
Publisher
共立出版
ISBN
9784320057852
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