Analysis of plant environmental response through plasmamembrane microdomain
Project/Area Number |
26292190
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Applied molecular and cellular biology
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Research Institution | Saitama University |
Principal Investigator |
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Project Period (FY) |
2014-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2017: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
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Keywords | 酸化ストレス / スフィンゴ脂質 / 脂質ラフト / 細胞死 / 環境ストレス応答 / 小胞体 / ストレス応答 / マイクロドメイン / シロイヌナズナ / イネ / 環境応答 / 細胞機能 / 脂質代謝 |
Outline of Final Research Achievements |
Plants respond to various environmental stresses through plasmamembrane microdomains. In our previous studied, we demonstrated that the overexpression of Arabidopsis BI-1, which localized on ER membrane, appeared enhanced tolerance to oxidative stress. In this research project, we characterized FAH, ELO, and SLD proteins, which are isolated as interacting factors to BI-1, directly or indirectly. Interestingly, these factors are related to the sphingolipid metabolism. The sphingolipid is known to be involved in the plasmamembrane microdomain. In this study, we produced transgenic plants in which these genes were over-expressing or knock-down. These plants showed modified sphingolipid profiles and different responses to oxidative stresses, suggest that the involvement of sphingolipid metabolism to the plat environmental responses.
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Academic Significance and Societal Importance of the Research Achievements |
植物は様々な環境ストレス下で生育しており、時として、大きなダメージを受ける。本研究では、モデル植物であるシロイヌナズナやイネを対象として、環境ストレスに対応するシグナル伝達因子が細胞膜上で局在して働くための足場を構成すると考えられているスフィンゴ脂質代謝系の因子に注目して研究を行った。その結果、それらの因子の機能を改変することにより、植物の環境ストレス感受性を変化させられる可能性を示した。本成果を作物に適用することにより、将来的に、食糧や生活に必要な植物資源の増産につながると考えている。
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Report
(6 results)
Research Products
(77 results)
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[Journal Article] Suppressing Arabidopsis GGLT1 affects growth by reducing the L-galactose content and borate cross-linking of rhamnogalacturonan II2018
Author(s)
Sechet J, Htwe S, Urbanowicz B, Agyeman A, Feng W, Ishikawa T, Dinneny J, Colomes M, Satish KK, Kawai-Yamada M, O'Neill M, Mortimer J
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Journal Title
The Plant Journal
Volume: 96
Issue: 5
Pages: 1036-1050
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) is a GlcNAc-containing glycosylinositol phosphorylceramide glycosyltransferase.2018
Author(s)
Ishikawa T, Fang L, Rennie EA, Sechet J, Yan J, Jing B, Moore W, Cahoon EB, Scheller HV, Kawai-Yamada M, Mortimer JC.
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Journal Title
Plant Physiol.
Volume: 177
Issue: 3
Pages: 938-952
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Effects of elevated atmospheric CO2 on respiratory rates in mature leaves of two rice cultivars grown at a free-air CO2 enrichment site and analyses of the underlying mechanisms.2018
Author(s)
Ko Noguchi, Tomonori Tsunoda, Atsuko Miyagi, Maki Kawai-Yamada, Daisuke Sugiura, Shin-Ichi Miyazawa, Takeshi Tokida, Yasuhiro Usui, Hirofumi Nakamura, Hidemitsu Sakai, Toshihiro Hasegawa
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Journal Title
Plant and Cell Physiology
Volume: 59
Issue: 3
Pages: 637-649
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] INT1 is a GIPC GlcNAc glycosyltransferase2018
Author(s)
Toshiki Ishikawa, Lin Fang, Emilie A. Rennie, Julien Sechet, Jingwei Yan, Beibei Jing, William Moore, Jonathan Markham, Edgar B. Cahoon, Henrik V. Scheller, Maki Kawai-Yamada1, Jenny C. Mortimer
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Journal Title
Plant Physiology
Volume: 未定
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Loss of inositol phosphorylceramide sphingolipid mannosylation induces plant immune responses and reduces cellulose content in Arabidopsis.2016
Author(s)
Fang L, Ishikawa T, Rennie EA, Murawska GM, Lao J, Yan J, Tsai AY, Baidoo EE, Xu J, Keasling JD, Demura T, Kawai-Yamada M, Scheller HV, *Mortimer JC
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Journal Title
Plant Cell
Volume: 28
Issue: 12
Pages: 2991-3004
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] KONJAC1 and 2 are key factors for GDP-mannose generation and affect l-ascorbic acid and glucomannan biosynthesis in Arabidopsis.2015
Author(s)
Sawake S, Tajima N, Mortimer JC, Lao J, Ishikawa T, Yu X, Yamanashi Y, Yoshimi Y, Kawai-Yamada M, Dupree P, Tsumuraya Y and Kotake T
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Journal Title
Plant Cell
Volume: 27
Issue: 12
Pages: 3397-3409
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Ethylene Biosynthesis Is Promoted by Very-Long-Chain Fatty Acids during Lysigenous Aerenchyma Formation in Rice Roots2015
Author(s)
Yamauchi T, Shiono K, Nagano M, Fukazawa A, Ando M, Takamure I, Mori H, Nishizawa NK, Kawai-Yamada M, Tsutsumi N, Kato K, Nakazono M
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Journal Title
Plant Physiology
Volume: 169
Issue: 1
Pages: 180-193
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Arabidopsis Bax inhibitor-1 promotes sphingolipid synthesis during cold stress by interacting with ceramide-modifying enzymes2014
Author(s)
Nagano, M., Ishikawa, T., Ogawa, Y., Iwabuchi, M., Nakasone, A., Shimamoto, K., Uchimiya, H., Kawai-Yamada, M.
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Journal Title
Planta
Volume: 240
Issue: 1
Pages: 77-89
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Rapid induction of lipid droplets in Chlamydomonas reinhardtii and Chlorella vulgaris by brefeldin A.2013
Author(s)
Kim, S., Kim, H., Ko, D., Yamaoka, Y., Otsuru, M., Kawai-Yamada, M., Ishikawa, T., Oh, H.-M., Nishida, I., Li-Beisson, Y., and Lee, Y.
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Journal Title
PLOS one
Volume: 8
Issue: 12
Pages: 295-304
DOI
Related Report
Peer Reviewed
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[Presentation] 組織特異的なクチクラ形成制御に基づいた種子の改変2017
Author(s)
Oshima Y, Narumi T, Kaneko Y, Ishikawa T, Kawai-Yamada M, Ohme-Takagi M, Mitsuda N
Organizer
植物生理学会
Place of Presentation
鹿児島大学(鹿児島県、鹿児島市)
Year and Date
2017-03-16
Related Report
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[Presentation] Sphingolipid-derived plasma membrane microdomains regulate innate immunity in rice.2014
Author(s)
Nagano, M., Ishikawa, T., Fujiwara, M., Fukao, Y., Kawano, Y., Kawai-Yamada, M.
Organizer
Plant Biology 2014
Place of Presentation
Portland (USA)
Year and Date
2014-07-12 – 2014-07-16
Related Report
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