| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2016: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
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| Outline of Final Research Achievements |
Cell wall polysaccharides such as pectin are known as major plant biomass. These are synthesized at the Golgi apparatus in the cell and are transported outside the cell via a vesicular transport system structure such as trans Golgi network (TGN).However the functional details and whether their function is controlled by nutrition had not been clarified.
Therefore, functional analysis of the Golgi apparatus was conducted mainly about the modification of glycoprotein. Analysis of structural transformation mechanism by TGN under sugar deficiency was also promoted. We also analyzed the localization and membrane orientation of proteins found in isolated fraction enriched with secretory vesicle cluster, which is one of the vesicular transport systems after TGN. These analyzes have deepened the accumulation of knowledge concerning the functions and control of the Golgi apparatus in plant cells.
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