Development of therapeutic systems based on three-dimensional DNA nanostructures
| Project/Area Number |
26293008
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Partial Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Physical pharmacy
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
Nishikawa Makiya 京都大学, 薬学研究科(研究院), 准教授 (40273437)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
高橋 有己 京都大学, 薬学研究科(研究院), 助教 (00547870)
高倉 喜信 京都大学, 薬学研究科(研究院), 教授 (30171432)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
ENDO Masayuki 京都大学, 物質-細胞統合システム拠点, 准教授 (70335389)
WATANABE Hiroshi 京都大学, 化学研究所, 教授 (90167164)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
|
|---|
| Keywords | 核酸 / DNA / DDS / ハイドロゲル / 抗原 |
|---|
| Outline of Final Research Achievements |
To develop therapeutic systems based on three-dimensional DNA nanostructures, we newly designed several types of basic DNA units. The comparison of their properties revealed that tetrapod-like structured DNA was the DNA nanostructure with the highest formation efficiency and uptake by immune cells. Then, a self-assembling gelling system was developed to obtain DNA hydrogel. Rheological analysis revealed that this hydrogel is injectable because of its quick gel-sol transition, and is regelated after injection. We also found that the antitumor effects of antigen-loaded DNA hydrogel can be greatly improved by the use of cationized antigen, which was more slowly released from the hydrogel than unmodified antigen.
|
Report
(4 results)
Research Products
(37 results)
-
-
[Journal Article] Self-assembling DNA hydrogel-based delivery of immunoinhibitory nucleic acids to immune cells.2016
Author(s)
Nishida Y, Ohtsuki S, Araie Y, Umeki Y, Endo M, Emura T, Hidaka K, Sugiyama H, Takahashi Y, Takakura Y, Nishikawa M.
-
Journal Title
Nanomedicine: Nanotechnology, Biology and Medicine
Volume: 12
Issue: 1
Pages: 123-130
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
-
-
-
-
[Journal Article] Optimal Arrangement of Four Short DNA Strands for Delivery of Immunostimulatory Nucleic Acids to Immune Cells2015
Author(s)
Shozo Ohtsuki, Noriyuki Matsuzaki, Kohta Mohri, Masayuki Endo, Tomoko Emura, Kumi Hidaka, Hiroshi Sugiyama, Yuki Takahashi, Kenichi Ishiyama, Norimitsu Kadowaki, Yoshinobu Takakura, Makiya Nishikawa.
-
Journal Title
Nucleic Acid Therapeutics
Volume: 25
Issue: 5
Pages: 245-253
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
-
-
-
[Journal Article] Ishii-Mizuno Y, Umeki Y, Takahashi Y, Kato Y, Takabayashi T, Fujieda S, Takakura Y, Nishikawa M. Nasal delivery of Japanese cedar pollen Cryj1 by using self-gelling immunostimulatory DNA for effective induction of immune responses in mice2015
Author(s)
Ishii-Mizuno Y, Umeki Y, Takahashi Y, Kato Y, Takabayashi T, Fujieda S, Takakura Y, Nishikawa M
-
Journal Title
Journal of Controlled Release
Volume: 200
Pages: 52-59
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
