| Project/Area Number |
26293008
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Kyoto University |
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Principal Investigator |
Nishikawa Makiya 京都大学, 薬学研究科(研究院), 准教授 (40273437)
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| Co-Investigator(Kenkyū-buntansha) |
高橋 有己 京都大学, 薬学研究科(研究院), 助教 (00547870)
高倉 喜信 京都大学, 薬学研究科(研究院), 教授 (30171432)
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| Co-Investigator(Renkei-kenkyūsha) |
ENDO Masayuki 京都大学, 物質-細胞統合システム拠点, 准教授 (70335389)
WATANABE Hiroshi 京都大学, 化学研究所, 教授 (90167164)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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| Keywords | 核酸 / DNA / DDS / ハイドロゲル / 抗原 |
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| Outline of Final Research Achievements |
To develop therapeutic systems based on three-dimensional DNA nanostructures, we newly designed several types of basic DNA units. The comparison of their properties revealed that tetrapod-like structured DNA was the DNA nanostructure with the highest formation efficiency and uptake by immune cells. Then, a self-assembling gelling system was developed to obtain DNA hydrogel. Rheological analysis revealed that this hydrogel is injectable because of its quick gel-sol transition, and is regelated after injection. We also found that the antitumor effects of antigen-loaded DNA hydrogel can be greatly improved by the use of cationized antigen, which was more slowly released from the hydrogel than unmodified antigen.
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