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Metallome analysis with cellular differentiation

Research Project

Project/Area Number 26293030
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Environmental and hygienic pharmacy
Research InstitutionChiba University

Principal Investigator

Ogra Yasumitsu  千葉大学, 大学院薬学研究院, 教授 (40292677)

Co-Investigator(Kenkyū-buntansha) 鈴木 紀行  千葉大学, 薬学研究科(研究院), 准教授 (10376379)
阿南 弥寿美  昭和薬科大学, 薬学部, 講師 (40403860)
小泉 信滋  独立行政法人労働者健康安全機構労働安全衛生総合研究所, 研究企画調整部, 特任研究員 (80183325)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2016: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Keywordsメタロミクス / スペシエーション / 分化 / 生体必須元素 / 銅 / セレン / 生体微量元素 / ICP-MS / イメージング / ESI-MS
Outline of Final Research Achievements

Metallomics is the name given to the integrated research field of biometals. Metallomics and research on metallome require analytical techniques that can provide information for the identification and quantification of metal species. The hitherto techniques for bio-trace elements including metals/metalloids have been applicable to massive samples such as blood serum, tissue extract and urine. However, analytical techniques for metallomics research using cultured cells are needed. Thus, in this study, we intended to reveal novel techniques for metallome analysis using cultured cells and novel concepts for the metabolism of bio-trace elements by the novel techniques. The first outcome in this study is the development of “element array” technique to analyze changes in entire elements with a cellular event. The second is to propose new reactive molecular species, “reactive cyanogen species” which act as a detoxificant of excess amounts of intracellular selenium.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (32 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (2 results) Journal Article (18 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 16 results,  Open Access: 7 results,  Acknowledgement Compliant: 15 results) Presentation (6 results) (of which Int'l Joint Research: 3 results,  Invited: 5 results) Book (3 results) Remarks (3 results)

  • [Int'l Joint Research] 浙江大学(中国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] バルパライソ神学大学(チリ)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Bioavailability comparison of nine bioselenocompounds in vitro and in vivo.2017

    • Author(s)
      K. Takahashi, N. Suzuki and Y. Ogra
    • Journal Title

      Int. J. Mol. Sci.

      Volume: - Issue: 3 Pages: 1-11

    • DOI

      10.3390/ijms18030506

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Comparison of metabolism of inorganic and organic selenium species between two selenium accumulator plants, garlic and Indian mustard.2017

    • Author(s)
      Y. Ogra, Y. Ogihara and Y. Anan
    • Journal Title

      Metallomics

      Volume: 9 Issue: 1 Pages: 61-68

    • DOI

      10.1039/c6mt00128a

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Biology and toxicology of tellurium explored by speciation analysis.2017

    • Author(s)
      Y. Ogra
    • Journal Title

      Metallomics

      Volume: - Issue: 5 Pages: 435-441

    • DOI

      10.1039/c7mt00022g

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Mechanisms underlying the toxic effects of antimony species in human embryonic kidney cells (HEK-293) and their comparison with arsenic species.2016

    • Author(s)
      M. Verdugo, Y. Ogra, and W. Quiroz
    • Journal Title

      J. Toxicol. Sci.

      Volume: 41 Pages: 783-792

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Changes in intracellular copper concentration and copper-regulating gene expression after PC12 differentiation into neurons.2016

    • Author(s)
      Y. Ogra, A. Tejima, N. Hatakeyama, M. Shiraiwa, S. Wu, T. Ishikawa, A. Yawata, Y. Anan and N. Suzuki
    • Journal Title

      Sci. Rep.

      Volume: 6 Issue: 1 Pages: 33007-33007

    • DOI

      10.1038/srep33007

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Metallomics approach to changes in element concentration during differentiation from fibroblasts into adipocytes by element array analysis.2016

    • Author(s)
      Y. Ogra, S. Nagasaki, A. Yawata, Y. Anan, K. Hamada and A. Mizutani
    • Journal Title

      J. Toxicol. Sci.

      Volume: 41 Pages: 241-244

    • NAID

      130005132657

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 生体内微量元素解析と機能解明2016

    • Author(s)
      小椋康光
    • Journal Title

      ぶんせき

      Volume: - Pages: 442-445

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 生命機能のファインチューナーとして働く生体微量元素研究の新たな動向2016

    • Author(s)
      小椋康光
    • Journal Title

      ファルマシア

      Volume: 52 Pages: 764-768

    • NAID

      130005254396

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detoxification of selenite to form selenocyanate in mammalian cells.2015

    • Author(s)
      Y. Anan, M. Kimura, M. Hayashi, R. Koike and Y. Ogra
    • Journal Title

      Chem. Res. Toxicol.

      Volume: 28 Issue: 9 Pages: 1803-1814

    • DOI

      10.1021/acs.chemrestox.5b00254

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Metabolism of trimethylselenonium ion in selenium accumulator, Allium sativum.2015

    • Author(s)
      Y. Ogra, Y. Ogihara and Y. Anan
    • Journal Title

      Fundam. Toxicol. Sci.

      Volume: 2 Pages: 95-99

    • NAID

      130005083365

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Complementary use of LC-ICP-MS and LC-ESI-Q-TOF-MS for selenium speciation.2015

    • Author(s)
      Y. Anan, G. Nakajima and Y. Ogra
    • Journal Title

      Anal. Sci.

      Volume: 31 Pages: 561-564

    • NAID

      130005075709

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Comparison of accumulation of four metalloids in Allium sativum.2015

    • Author(s)
      Y. Ogra, Y. Awaya and Y. Anan
    • Journal Title

      Bull. Environ. Contam. Toxicol.

      Volume: 94 Issue: 5 Pages: 604-608

    • DOI

      10.1007/s00128-015-1508-6

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Complementary use of LC-ICP-MS and LC-ESI-Q-TOF-MS for selenium Speciation.2015

    • Author(s)
      Y. Anan, G. Nakajima and Y. Ogra
    • Journal Title

      Anal. Sci.

      Volume: in press

    • NAID

      130005075709

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 環境毒性学におけるメタロミクス研究の展開2015

    • Author(s)
      小椋康光
    • Journal Title

      YAKUGAKU ZASSHI

      Volume: 135 Pages: 307-314

    • NAID

      130004756488

    • Related Report
      2014 Annual Research Report
  • [Journal Article] An improved tolerance to cadmium by overexpression of two genes for cysteine synthesis in tobacco.2014

    • Author(s)
      M. Nakamura, T. Ochiai, M. Noji, Y. Ogra, K.T. Suzuki, N. Yoshimoto, M. Yamazaki and K. Saito
    • Journal Title

      Plant Biotech.

      Volume: 31 Pages: 141-147

    • NAID

      130003391375

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Arsenic (+3 oxidation state) methyltransferase is a specific but replaceable factor against arsenic toxicity.2014

    • Author(s)
      M. Tokumoto, N. Kutsukake, E. Yamanishi, D. Katsuta, Y. Anan and Y. Ogra
    • Journal Title

      Toxicol. Rep.

      Volume: 1 Pages: 589-595

    • DOI

      10.1016/j.toxrep.2014.08.011

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Metabolic pathway of inorganic and organic selenocompounds labeled with stable isotope in Japanese quail.2014

    • Author(s)
      Anan Y, Ohbo A, Tani Y, Ogra Y.
    • Journal Title

      Anal Bioanal Chem

      Volume: 406 Issue: 30 Pages: 7959-7966

    • DOI

      10.1007/s00216-014-8260-3

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 哺乳類細胞における銅の恒常性維持の分子機構2014

    • Author(s)
      小椋康光
    • Journal Title

      日本衛生学会誌

      Volume: 69 Pages: 136-145

    • NAID

      130004776102

    • Related Report
      2014 Annual Research Report
  • [Presentation] Evaluation of naturally occurring selenocompounds on their nutritional availability.2016

    • Author(s)
      Y. Ogra, K. Takahashi and N. Suzuki
    • Organizer
      The International Selenium Seminar (ISS 2016)
    • Place of Presentation
      Guangzhou-Shenzhen, China
    • Year and Date
      2016-09-21
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 質量分析法を基盤としたセレノメタボローム解析2016

    • Author(s)
      小椋康光
    • Organizer
      第43回BMSコンファレンス
    • Place of Presentation
      熱海、静岡
    • Year and Date
      2016-07-05
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Biosynthesis and biological role of selenocyanate.2015

    • Author(s)
      Y. Ogra
    • Organizer
      The 5th International Selenium Seminar 2015
    • Place of Presentation
      Yaroslavl, Russia
    • Year and Date
      2015-09-21
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Identification of a novel selenium metabolite and elucidation of its biological and toxicological roles.2015

    • Author(s)
      Y. Ogra
    • Organizer
      5th International Symposium on Metallomics
    • Place of Presentation
      Beijing, China
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Identification of a novel selenium metabolite in cultured cells and evaluation of its biological function.2014

    • Author(s)
      Y. Ogra
    • Organizer
      The International Se Seminar 2014
    • Place of Presentation
      Busan, Korea
    • Year and Date
      2014-10-16 – 2014-10-18
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Changes in intracellular copper concentration and expression of copper-regulating genes after differentiation of rat pheochromocytoma (PC12) cells into neural cells.2014

    • Author(s)
      Y. Ogra, M. Tokumoto, A. Tejima, N.o Hatakeyama, S. Wu, T. Ishikawa, N. Suzuki, Y. Anan
    • Organizer
      17th BNASS/14th TraceSpec Tandem Conference
    • Place of Presentation
      Aberdeen, UK
    • Year and Date
      2014-07-31 – 2014-08-04
    • Related Report
      2014 Annual Research Report
  • [Book] Metallomics2017

    • Author(s)
      Y. Ogra and T. Hirata
    • Total Pages
      400
    • Publisher
      Springer
    • Related Report
      2016 Annual Research Report
  • [Book] Diversity of Selenium Functions in Health and Disease2015

    • Author(s)
      Y. Ogra
    • Total Pages
      400
    • Publisher
      CRC Press
    • Related Report
      2015 Annual Research Report
  • [Book] Food and Nutritional Components in Focus No. 9, Selenium: Chemistry, Analysis, Function and Effects2015

    • Author(s)
      Y. Ogra
    • Total Pages
      642
    • Publisher
      The Royal Society of Chemistry
    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
  • [Remarks] 千葉大学大学院薬学研究院 予防薬学研究室

    • URL

      http://www.p.chiba-u.jp/lab/yobou/index.html

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
  • [Remarks] 昭和薬科大学 衛生化学研究室 OGRA LAB

    • URL

      http://ogra-lab.com/

    • Related Report
      2014 Annual Research Report
  • [Remarks] 千葉大学大学院薬学研究院 予防薬学研究室 OGRA LAB

    • URL

      http://www.p.chiba-u.jp/lab/yobou/index.html

    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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