| Budget Amount *help |
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2016: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
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| Outline of Final Research Achievements |
In this study, we developed the novel DNA vaccines against the neglected tropical diseases (NTD). At first, we tried to construct the DNA vector suitable for DNA vaccine. We successfully prepared the spleen-targeted gene vector by optimization of the mix ratio of pDNA, dendrigraft poly-L-lysine (DGL), and γ-polyglutamic acid (γ-PGA) and the preparation process. We applied the vector to malaria DNA vaccines. Malaria DNA nano-vaccine showed high immune induction and prolonged survival rate of malaria-infected mice. Furthermore, we applied the vector to schistosomiasis DNA vaccine. As results, schistosomiasis DNA nano-vaccine induced dominant Th1 cellular immune response and markedly reduced the egg burden in the liver.
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