Development of the novel technique intended to the prophylaxis and treatment of neglected tropical diseases
| Project/Area Number |
26293034
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical pharmacy
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| Research Institution | Nagasaki University |
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Principal Investigator |
SASAKI Hitoshi 長崎大学, 病院(医学系), 教授 (00170689)
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| Co-Investigator(Kenkyū-buntansha) |
太田 伸生 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (10143611)
平山 謙二 長崎大学, 熱帯医学研究所, 教授 (60189868)
濱野 真二郎 長崎大学, 熱帯医学研究所, 教授 (70294915)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2016: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
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| Keywords | 遺伝子ベクター / 顧みられない熱帯病 / マラリア / 住血吸虫症 / シャーガス病 / DNAワクチン / 感染症 / 遺伝子 / 核酸 / ナノデバイス |
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| Outline of Final Research Achievements |
In this study, we developed the novel DNA vaccines against the neglected tropical diseases (NTD). At first, we tried to construct the DNA vector suitable for DNA vaccine. We successfully prepared the spleen-targeted gene vector by optimization of the mix ratio of pDNA, dendrigraft poly-L-lysine (DGL), and γ-polyglutamic acid (γ-PGA) and the preparation process. We applied the vector to malaria DNA vaccines. Malaria DNA nano-vaccine showed high immune induction and prolonged survival rate of malaria-infected mice. Furthermore, we applied the vector to schistosomiasis DNA vaccine. As results, schistosomiasis DNA nano-vaccine induced dominant Th1 cellular immune response and markedly reduced the egg burden in the liver.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Quaternary complexes modified from pDNA and poly-l-lysine complexes to enhance pH-buffering effect and suppress cytotoxicity.2015
Author(s)
Kodama Y, Yatsugi Y, Kitahara T, Kurosaki T, Egashira K, Nakashima M, Muro T, Nakagawa H, Higuchi N, Nakamura T, Sasaki H.
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Journal Title
Journal of Pharmaceutical Sciences
Volume: 104
Issue: 4
Pages: 1470-1477
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Secure and effective gene delivery system of plasmid DNA coated by polynucleotide.2015
Author(s)
Kodama Y, Ohkubo C, Kurosaki T, Egashira K, Sato K, Fumoto S, Nishida K, Higuchi N, Kitahara T, Nakamura T, Sasaki H.
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Journal Title
J Drug Target.
Volume: 23
Issue: 1
Pages: 43-51
DOI
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Immunogenicity and anti-fecundity effect of nanoparticle coated glutathione S-transferase (SjGST) DNA vaccine against murine Schistosoma japonicum infection.2015
Author(s)
Mbanefo EC, Kumagai T, Kodama Y, Kurosaki T, Furushima-Shimogawara R, Cherif MS, Mizukami S, Kikuchi M, Huy NT, Ohta N, Sasaki H, Hirayama K.
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Journal Title
Parasitol Int.
Volume: 64
Issue: 4
Pages: 24-31
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Splenic gene delivery system using self-assembling nano-complex with phosphatidylserine analog.2015
Author(s)
Kurosaki T, Nakasone C, Kodama Y, Egashira K, Harasawa H, Muro T, Nakagawa H, Kitahara T, Higuchi N, Nakamura T, Sasaki H.
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Journal Title
Biol Pharm Bull.
Volume: 38
Pages: 23-29
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
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