• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Study on the structure and function of glycans in target proteins for moving toward individualized medicine using antibody drugs

Research Project

Project/Area Number 26293037
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Medical pharmacy
Research InstitutionYokohama City University (2015-2016)
National Institute of Health Sciences (2014)

Principal Investigator

Kawasaki Nana  横浜市立大学, 生命医科学研究科, 教授 (20186167)

Co-Investigator(Kenkyū-buntansha) 小川 久美子  国立医薬品食品衛生研究所, 病理部, 部長 (70254282)
多田 稔  国立医薬品食品衛生研究所, 生物薬品部, 室長 (50506954)
石井 明子  国立医薬品食品衛生研究所, 生物薬品部, 室長 (50291117)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2014: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Keywords糖鎖 / 質量分析 / EGF受容体 / 抗体医薬品 / 個別化医療 / 薬効 / 体内動態
Outline of Final Research Achievements

For individualized medicine using monoclonal antibody drugs (mAbs), it is important to clarify the diversity of target molecules for mAbs and the relationship between the diversity and efficacy and safety of mAb products. To elucidate the glycan diversity on target proteins for mAbs, we developed an analytical method for the site-specific glycosylation of membrane proteins; this method comprised the extraction of target proteins from the membrane fraction by immunoprecipitation with mAbs, trypsin digestion, glycopeptide enrichment by acetone precipitation, and LC/MS. Using the method, we successfully demonstrated the glycosylation at 5 sites of EGFR in A431 cells. It was suggested that Asn 528 is attached to unique glycans bearing both 7-9 GlcNAc and 2-6 Fuc residues. We also showed the site-specific glycosylation of the Fc gamma receptor IIIb expressed in Baby hamster kidney cells and in human serum.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (22 results)

All 2016 2015 2014 Other

All Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 4 results,  Acknowledgement Compliant: 3 results) Presentation (12 results) (of which Invited: 2 results) Book (3 results) Remarks (1 results)

  • [Journal Article] Characterization of Glycoproteins Expressing the Blood Group H Type 1 Epitope on Human Induced Pluripotent Stem (hiPS) Cells2016

    • Author(s)
      Nakao, H., Matsumoto, S., Nagai, Y., Kojima, A., Toyoda, H., Hashii, N., Takakura, D., Kawasaki, N., Yamaguchi, T., Kawabata, K., Kawasaki, N., Kawasaki, T.
    • Journal Title

      Glycoconjugate J

      Volume: 19 Issue: 6 Pages: 1-9

    • DOI

      10.1007/s10719-016-9710-2

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sialylation converts arthritogenic IgG into inhibitors of collagen-induced arthritis.2016

    • Author(s)
      Ohmi Y, Ise W, Harazono A, Takakura D, Fukuyama H, Baba Y, Narazaki M, Shoda H, Takahashi N, Ohkawa Y, Ji S, Sugiyama F, Fujio K, Kumanogoh A, Yamamoto K, Kawasaki N, Kurosaki T, Takahashi Y, Furukawa K.
    • Journal Title

      Nature Communications

      Volume: in press Issue: 1 Pages: 11205-11205

    • DOI

      10.1038/ncomms11205

    • NAID

      120007135549

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Membrane glycoproteomics of fetal lung fibroblasts using LC/MS.2016

    • Author(s)
      D. Takakura, M. Tada, and N. Kawasaki
    • Journal Title

      Proteomics

      Volume: 16 Issue: 1 Pages: 47-59

    • DOI

      10.1002/pmic.201500003

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Comparison of analytical methods for profiling N- and O-linked glycans from cultured cell lines. HUPO Human Disease Glycomics/Proteome Initiative multi-institutional study.2015

    • Author(s)
      Ito H,Kaji H,Togayachi A,Azadi P,Ishihara M,Geyer R, Galuska C,Geyer H,Kakehi K,Kinoshita M,Karlsson NG,Jin C,Kato K,Yagi H,Kondo S,Kawasaki N,Hashii N,Kolarich D,Stavenhagen K,Packer NH,Thaysen-Andersen M,Nakano M,Taniguchi N,Kurimoto A,Wada Y,Tajiri M,Yang P,Cao W,Li H,Rudd PM,Narimatsu H.
    • Journal Title

      Glycoconjugate J

      Volume: Oct 28

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] バイオ医薬品の現状と展望2015

    • Author(s)
      石井明子,川崎ナナ
    • Journal Title

      ファルマシア

      Volume: 51 Pages: 403-407

    • NAID

      130007448152

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Site-Specific Classification of N-Linked Oligosaccharides of the Extracellular Regions of Fcg Receptor IIIb Expressed in Baby Hamster Kidney Cells.2014

    • Author(s)
      Kawasaki N, Okumoto T, Yamaguchi Y, Takahashi N, Fridman WH, Sautѐs-Fridman C, Yagi H, Kato K
    • Journal Title

      J. Glycomics Lipidomics

      Volume: 4(2) Pages: 1000116-1000116

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 乳がん細胞膜糖タンパク質の部位特異的なN-型糖鎖変化2016

    • Author(s)
      吉田晴香、高倉大輔、川崎ナナ
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] 糖鎖特異的ヒトiPS細胞傷害性抗体の認識するiPS 細胞糖タンパク質の解析2016

    • Author(s)
      川嵜伸子、中尾広美、永井裕子、小嶋絢、豊田英尚、橋井則貴、高倉大輔、川崎ナナ、山口朋子、川端健二、川嵜敏祐
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      仙台国際センター(宮城県・仙台市)
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] 関節リウマチ抗原特異的抗体上のシアル酸はII型コラーゲン誘導型関節炎を抑制する2016

    • Author(s)
      大海雄介、伊勢渉、原園景、高倉大輔、福山英啓、川崎ナナ、河原敏男、黒崎知博、高橋宜聖、古川鋼一
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      仙台国際センター(宮城県・仙台市)
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] 関節炎惹起性IgGのシアル酸修飾はコラーゲン誘導型関節炎の抑制機能を付与する2016

    • Author(s)
      大海雄介、伊勢渉、原園景、高倉大輔、福山英啓、川崎ナナ、河原敏男、黒崎知博、高橋宜聖、古川鋼一
    • Organizer
      第35回日本糖質学会年会
    • Place of Presentation
      高知市文化プラザ かるぽーと(高知県・高知市)
    • Year and Date
      2016-09-01
    • Related Report
      2016 Annual Research Report
  • [Presentation] ヒト血清由来の可溶型Fc&#61543レセプターIIIbの部位特異的なN型糖鎖の構造解析2016

    • Author(s)
      矢木宏和、高倉大輔、Lubka T. Roumenina、 Wolf Herman Fridman、Catherine Sautès-Fridman、川崎ナナ、加藤晃一
    • Organizer
      第35回日本糖質学会年会
    • Place of Presentation
      高知市文化プラザ かるぽーと(高知県・高知市)
    • Year and Date
      2016-09-01
    • Related Report
      2016 Annual Research Report
  • [Presentation] 乳がん細胞糖タンパク質のミクロ不均一性変化の解析2016

    • Author(s)
      高倉大輔、吉田晴香、川崎ナナ
    • Organizer
      第35回日本糖質学会年会
    • Place of Presentation
      高知市文化プラザ かるぽーと(高知県・高知市)
    • Year and Date
      2016-09-01
    • Related Report
      2016 Annual Research Report
  • [Presentation] グライコプロテオミクス技術による乳がん細胞中の糖タンパク質の腫瘍関連グリコフォーム解析2016

    • Author(s)
      髙倉大輔、吉田晴香、川崎ナナ
    • Organizer
      日本プロテオーム学会2016年会
    • Place of Presentation
      北里大学薬学部(東京都・港区)
    • Year and Date
      2016-07-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] 質量分析による生体糖タンパク質解析のフロンティア.2016

    • Author(s)
      川崎ナナ
    • Organizer
      第64回質量分析総合討論会
    • Place of Presentation
      ホテル阪急エキスポパーク(大阪府・吹田市)
    • Year and Date
      2016-05-18
    • Related Report
      2016 Annual Research Report
  • [Presentation] 膜グライコミクス技術の確立2015

    • Author(s)
      高倉大輔,川崎ナナ
    • Organizer
      糖質学会
    • Place of Presentation
      東京
    • Year and Date
      2015-07-31
    • Related Report
      2015 Annual Research Report
  • [Presentation] 糖タンパク質分析技術の開発2015

    • Author(s)
      川崎ナナ,高倉大輔
    • Organizer
      日本プロテオーム学会
    • Place of Presentation
      熊本
    • Year and Date
      2015-07-23
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 躍進する抗体医薬品-現状と課題2014

    • Author(s)
      川崎ナナ
    • Organizer
      第58回日本薬学会関東支部大会
    • Place of Presentation
      東京
    • Year and Date
      2014-10-04
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] アセトン濃縮とLC/MSによる糖ペプチドの選択的プロファイリングと膜グライコミクスへの応用2014

    • Author(s)
      高倉大輔,多田 稔,川崎ナナ
    • Organizer
      日本プロテオーム学会2014年会
    • Place of Presentation
      つくば
    • Year and Date
      2014-07-17 – 2014-07-18
    • Related Report
      2014 Annual Research Report
  • [Book] 日本薬局方技術情報2016 (JPTI 2016), 一般試験法「糖鎖試験法」2016

    • Author(s)
      川崎ナナ
    • Total Pages
      1388
    • Publisher
      株式会社じほう
    • Related Report
      2016 Annual Research Report
  • [Book] 日本薬局方技術情報2016 (JPTI 2016), 参考情報「単糖分析及びオリゴ糖分析/糖鎖プロファイル法」2016

    • Author(s)
      川崎ナナ
    • Total Pages
      1388
    • Publisher
      株式会社じほう
    • Related Report
      2016 Annual Research Report
  • [Book] 日本薬局方技術情報2016 (JPTI 2016), 参考情報「ペプチド及びタンパク質の質量分析」2016

    • Author(s)
      川崎ナナ
    • Total Pages
      1388
    • Publisher
      株式会社じほう
    • Related Report
      2016 Annual Research Report
  • [Remarks] 創薬再生科学研究室 川崎ナナグループ - 横浜市立大学鶴見キャンパス

    • URL

      http://www.tsurumi.yokohama-cu.ac.jp/proteome/nana/japanese/index.html

    • Related Report
      2016 Annual Research Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi