| Budget Amount *help |
¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2015: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2014: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Outline of Final Research Achievements |
We expressed GFP protein in the astrocytes of the Globus Palidus (GP) by double transgenic technology, which utilize Olig2 gene promoter-driven Cre recombinnase and ROSA-floxed-EGFP. Voluntary exercise (running) for three weeks renders the Olig2-astrocytes more complex and bushy morphologies than those of control (sedated) mice. These bushy astrocytes may be involved in synaptic transmission by promoting transmitter uptake and/or by releasing gliotransmitters. To test this hypothesis, we expressed channerhodopsin in the Olig2-astrocytes in the unilateral GP. By introducing blue laser beam, we specifically activated the astrocytes and checked effects on the mice behavior. The laser beam-treatment induced circling behavior in the double transgenic mice, suggesting that there might be invalance between ipsilateral and contralateral neural circuit activities of the basal ganglia. We are now investigating detailed molecular mechanismas underlying this behavior.
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