Morphology-function relationship in the astrocytes of Globus Pallidus

• Project/Area Number: 26293039
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Nara Medical University
• Principal Investigator: WANAKA AKIO 奈良県立医科大学, 医学部, 教授 (90210989)
• Co-Investigator(Kenkyū-buntansha): 奥田 洋明 奈良県立医科大学, 医学部, 助教 (40453162) ; 森田 晶子 奈良県立医科大学, 医学部, 助教 (70647049)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000); Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2015: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2014: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: アストロサイト / 大脳基底核 / 運動 / 光遺伝学 / シナプス伝達 / Olig2 / GFAP / 三つ組みシナプス / パーキンソン病 / アデノ随伴ウイルス / 淡蒼球 / 黒質網様部 / マウス / シナプス / チャネロドプシン / ハロロドプシン

Outline of Final Research Achievements

We expressed GFP protein in the astrocytes of the Globus Palidus (GP) by double transgenic technology, which utilize Olig2 gene promoter-driven Cre recombinnase and ROSA-floxed-EGFP. Voluntary exercise (running) for three weeks renders the Olig2-astrocytes more complex and bushy morphologies than those of control (sedated) mice. These bushy astrocytes may be involved in synaptic transmission by promoting transmitter uptake and/or by releasing gliotransmitters. To test this hypothesis, we expressed channerhodopsin in the Olig2-astrocytes in the unilateral GP. By introducing blue laser beam, we specifically activated the astrocytes and checked effects on the mice behavior. The laser beam-treatment induced circling behavior in the double transgenic mice, suggesting that there might be invalance between ipsilateral and contralateral neural circuit activities of the basal ganglia. We are now investigating detailed molecular mechanismas underlying this behavior.

Research Products

• [Journal Article] Microglia-derived neuregulin expression in psychiatric disorders. (2017)
  • Author(s): Ikawa D, Makinodan M, Iwata K, Ohgidani M, Kato TA, Yamashita Y, Yamamuro K, Kimoto S, Toritsuka M, Yamauchi T, Fukami SI, Yoshino H, Okumura K, Tanaka T, Wanaka A, Owada Y, Tsujii M, Sugiyama T, Tsuchiya K, Mori N, Hashimoto R, Matsuzaki H, Kanba S, Kishimoto T.
  • Journal Title: Brain Behav Immun. Volume: 61 Pages: 375-385
  • DOI: 10.1016/j.bbi.2017.01.003
  • Peer Reviewed / Open Access
• [Journal Article] Hedgehog Signaling Components Are Expressed in Choroidal Neovascularization in Laser-induced Retinal Lesion (2016)
  • Author(s): Nochioka K, Okuda H, Tatsumi K, Morita S, Ogata N, Wanaka A.
  • Journal Title: ACTA HISTOCHEMICA ET CYTOCHEMICA Volume: 49 Issue: 2 Pages: 67-74
  • DOI: 10.1267/ahc.15036
  • NAID: 130005149556
  • ISSN: 0044-5991, 1347-5800
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Voluntary Exercise Induces Astrocytic Structural Plasticity in the Globus Pallidus. (2016)
  • Author(s): Tatsumi K, Okuda H, Morita-Takemura S, Tanaka T, Isonishi A, Shinjo T, Terada Y, Wanaka A.
  • Journal Title: Front Cell Neurosci. Volume: 10 Pages: 165-165
  • DOI: 10.3389/fncel.2016.00165
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A subtype of olfactory bulb interneurons is required for odor detection and discrimination behaviors (2016)
  • Author(s): Takahashi H, Ogawa Y, Yoshihara S, Asahina R, Kinoshita M, Kitano T, Kitsuki M, Tatsumi K, Okuda M, Tatsumi K, Wanaka A, Hirai H, Stern PL and Tsuboi A
  • Journal Title: The Journal of Neuroscience Volume: 36 Issue: 31 Pages: 8210-8227
  • DOI: 10.1523/jneurosci.2783-15.2016
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Changes in endothelial cell proliferation and vascular permeability after systemic lipopolysaccharide administration in the subfornical organ. (2016)
  • Author(s): Morita-Takemura S, Nakahara K, Tatsumi K, Okuda H, Tanaka T, Isonishi A, Wanaka A.
  • Journal Title: J Neuroimmunol. Volume: 298 Pages: 132-137
  • DOI: 10.1016/j.jneuroim.2016.06.011
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Social isolation impairs remyelination in mice through modulation of IL-6. (2016)
  • Author(s): Makinodan M, Ikawa D, Miyamoto Y, Yamauchi J, Yamamuro K, Yamashita Y, Toritsuka M, Kimoto S, Okumura K, Yamauchi T, Fukami SI, Yoshino H, Wanaka A, Kishimoto T.
  • Journal Title: FASEB J. Volume: 30 Pages: 4267-4274
  • Peer Reviewed
• [Journal Article] Heterogenous vascular permeability and alternative diffusion barrier in the sensory circumventricular organs of adult mouse brain (2015)
  • Author(s): Morita, S, Furube, E, Mannari, T, Okuda, H, Tatsumi, K, Wanaka, A, Miyata, S
  • Journal Title: Cell and Tissue Research Volume: 0 Issue: 2 Pages: 0-0
  • DOI: 10.1007/s00441-015-2207-7
  • Peer Reviewed / Open Access
• [Journal Article] Hedgehog Signaling Modulates the Release of Gliotransmitters from Cultured Cerebellar Astrocytes. (2015)
  • Author(s): Okuda H, Tatsumi K, Morita-Takemura S, Nakahara K, Nochioka K, Shinjo T, Terada Y, Wanaka A
  • Journal Title: Neurochem Res Volume: 41 Issue: 1-2 Pages: 278-89
  • DOI: 10.1007/s11064-015-1791-y
  • Peer Reviewed
• [Journal Article] Retinoic acid regulates Lhx8 expression via FGF-8b to the upper jaw development of chick embryo (2015)
  • Author(s): Shimomura T, Kawakami M, Okuda H,Tatsumi K, Morita S, Nochioka K, Kirita T, Wanaka A.
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 119 Issue: 3 Pages: 260-266
  • DOI: 10.1016/j.jbiosc.2014.08.010
  • NAID: 110009923564
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Vascular endothelial growth factor-dependent angiogenesis and dynamic vascular plasticity in the sensory circumventricular organs of adult mouse brain (2015)
  • Author(s): Morita, S, Furube, E, Mannari, T, Okuda, H, Tatsumi, K, Wanaka, A, Miyata, S
  • Journal Title: Cell and Tissue Research Volume: 359 Issue: 3 Pages: 865-884
  • DOI: 10.1007/s00441-014-2080-9
  • Peer Reviewed
• [Journal Article] Astrocytic TLR4 expression and LPS-induced nuclear translocation of STAT3 in the sensory circumventricular organs of adult mouse brain (2015)
  • Author(s): Nakano, Y, Furube, E, Morita, S, Wanaka, A, Nakashima, T, Miyata, S
  • Journal Title: Journal of Neuroimmunology Volume: 278 Pages: 144-158
  • DOI: 10.1016/j.jneuroim.2014.12.013
  • Peer Reviewed
• [Journal Article] OASIS regulates chondroitin 6-O-sulfotransferase 1 gene transcription in the injured adult mouse cerebral cortex (2014)
  • Author(s): Okuda H, Tatsumi K, Horii-Hayashi N, Morita S, Okuda-Yamamoto A, Imaizumi K, Wanaka A
  • Journal Title: J Neurochem Volume: Epub ahead Issue: 5 Pages: 612-25
  • DOI: 10.1111/jnc.12736
  • Peer Reviewed
• [Journal Article] Geissoschizine methyl ether, an alkaloid from the Uncaria hook, improves remyelination after cuprizone-induced demyelination in medial prefrontal cortex of adult mice. (2014)
  • Author(s): Morita S, Tatsumi K, Makinodan M, Okuda H, Kishimoto T, Wanaka A.
  • Journal Title: Neurochem Res. Volume: 39 Issue: 1 Pages: 59-67
  • DOI: 10.1007/s11064-013-1190-1
  • Peer Reviewed
• [Journal Article] Chondroitin sulfate proteoglycan tenascin-R regulates glutamate uptake by adult brain astrocytes (2014)
  • Author(s): Okuda H, Tatsumi K, Morita S, Shibukawa Y, Korekane H, Horii-Hayashi N, Wada Y, Taniguchi N, Wanaka A.
  • Journal Title: J Biol Chem Volume: 289 Issue: 5 Pages: 2620-2631
  • DOI: 10.1074/jbc.m113.504787
  • Peer Reviewed / Open Access
• [Journal Article] Inhibition of Wnt/β-catenin pathway by Dikkopf-1 affects midfacial morphogenesis in chick embryo. (2014)
  • Author(s): Kawakami M, Okuda H, Tatsumi K, Kirita T, Wanaka A.
  • Journal Title: J Biosci Bioeng. Volume: 117 Issue: 6 Pages: 664-669
  • DOI: 10.1016/j.jbiosc.2013.11.015
  • NAID: 110009823147
  • Peer Reviewed
• [Presentation] Astrocytic structural changes correlate with overall running activities in the globus pallidus (2016)
  • Author(s): Tatsumi K, Okuda H, Takemura SM, Tanaka T, Isonishi A, Wanaka A
  • Organizer: Front Cell Neurosci
  • Place of Presentation: マレーシア、クアラルンプール
  • Year and Date: 2016-08-27
  • Int'l Joint Research
• [Presentation] Hedgehog signaling modulates the release of gliotransmitters from cultured cerebellar astrocytes. (2015)
  • Author(s): 奥田洋明、辰巳晃子、森田晶子、和中明生
  • Organizer: 第58回日本神経化学会大会
  • Place of Presentation: 大宮ソニックシティ
  • Year and Date: 2015-09-11
• [Presentation] Characterization of Olig2!positive astro- cytes in the normal adult forebrain (2015)
  • Author(s): 辰巳晃子、奥田洋明、森田晶子、和中明生
  • Organizer: 第58回日本神経化学会大会
  • Place of Presentation: 大宮ソニックシティ
  • Year and Date: 2015-09-11
• [Presentation] Astrocytic chondroitin sulfate proteoglycans in brain injury and in glutamate uptake funcions (2015)
  • Author(s): Akio Wanaka, Hiroaki Okuda, Kouko Tatsumi, Shoko Morita
  • Organizer: 25th Meeting of the International Society for Neurochemistry
  • Place of Presentation: Cairns, Australia
  • Year and Date: 2015-08-23
  • Int'l Joint Research
• [Presentation] 末梢からの炎症シグナルによって引き起こされる脳弓下器官の血管の可塑的変化 (2015)
  • Author(s): 森田晶子、中原一貴、辰巳晃子、奥田洋明、宮田清司、和中明生
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2015-07-28
• [Presentation] 統合失調症モデルマウスに対する抑肝散及びその構成成分の薬理効果 (2014)
  • Author(s): 和中明生、森田晶子、奥田洋明、牧之段学、辰巳晃子、岸本年史
  • Organizer: 第５7回日本神経化学会大会
  • Place of Presentation: 奈良県新公会堂、奈良市
  • Year and Date: 2014-09-29 – 2014-10-01
• [Presentation] 神経活動に伴うアストロサイトの形態変化 (2014)
  • Author(s): 辰巳晃子、奥田洋明、森田晶子、和中明生
  • Organizer: 第５7回日本神経化学会大会
  • Place of Presentation: 奈良県文化会館、奈良市
  • Year and Date: 2014-09-29 – 2014-10-01
• [Presentation] 神経活動にともなうアストロサイトの形態変化 (2014)
  • Author(s): 辰巳晃子、奥田洋明、森田晶子、和中明生
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: パシフィコ横浜、横浜市
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] アストロサイトにおけるヘッジホッグシグナルを介したGLASTの発現調節 (2014)
  • Author(s): 奥田洋明、辰巳晃子、森田晶子、和中明生
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: パシフィコ横浜、横浜市
  • Year and Date: 2014-09-11 – 2014-09-13