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Three dimensional ultrastructural analysis of fibroblasts and their cellular network in the connective tissue by FIB/SEM tomography

Research Project

Project/Area Number 26293040
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field General anatomy (including histology/embryology)
Research InstitutionKurume University

Principal Investigator

NAKAMURA KEIICHIRO  久留米大学, 医学部, 教授 (20172398)

Co-Investigator(Kenkyū-buntansha) 太田 啓介  久留米大学, 医学部, 准教授 (00258401)
林 篤正  久留米大学, 医学部, 講師 (20341357)
金澤 知之進  久留米大学, 医学部, 講師 (50529518)
東 龍平  久留米大学, 医学部, その他 (70569516)
都合 亜記暢  久留米大学, 医学部, その他 (80569517)
力丸 由起子 (西由起子 / 力丸 由起子(西由起子))  久留米大学, 医学部, 助教 (90368960)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
Keywords線維芽細胞 / 結合組織 / 細胞ネットワーク / 三次元微細構造 / FIB/SEM / 電子顕微鏡 / 間質細胞 / 新機軸電子顕微鏡 / FIB/SEM tomography / 移植・再生医療 / 細胞・組織 / 発生・分化 / 間葉 / 間質組織 / 平滑筋 / 間葉細胞 / ブロック表面SEM観察(BFI) / 次世代電子顕微鏡 / 三次元超微形態
Outline of Final Research Achievements

In order to reevaluate the connective tissue cellular construction, we have performed FIB/SEM tomography analysis; a novel election microscope technique that enables to obtain serial fine microscopic images and three dimensional ultrastructural analysis by reconstructing structures on image analyzing PC system, upon dermal tissue of the skin, wall of urogenital organs, tendon-bone junction, and periodontal ligaments, following the ordinary histological observations. As results, our FIB/SEM tomographic observations have revealed that the cells usually show very thin, flat, and undulated sheet-like shape, although the shape usually described as spindle or dendritic with one or more thin processes. Those cells are connected one another to form cellular network, and the flat surface of them divide the connective tissue space into small compartments (compartmentalization), which could play important functional roles for biomedical phenomenon.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report

Research Products

(82 results)

All 2017 2016 2015 2014

All Journal Article (26 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 23 results,  Open Access: 11 results,  Acknowledgement Compliant: 3 results) Presentation (56 results) (of which Int'l Joint Research: 4 results,  Invited: 3 results)

  • [Journal Article] Interstitial cell modulation of pyeloureteric peristalsis in the mouse renal pelvis examined using FIBSEM tomography and calcium indicators2017

    • Author(s)
      Hashitani Hikaru, Michael Nguyen, Haruka Noda, Retsu Mitsui, Ryuhei Higashi, Keisuke Ohta, Kei-Ichiro Nakamura, Richard Lang
    • Journal Title

      Pfluegers Archiv - European Journal of Physiology

      Volume: 469 Pages: 797-813

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Three-dimensional analysis of somatic mitochondrial dynamics in fission-deficient injured motor neurons using FIB/SEM2017

    • Author(s)
      Tamada H, Kiryu-Seo S, Hosokawa H, Ohta K, Ishihara N, Nomura M, Mihara K, Nakamura KI, Kiyama H
    • Journal Title

      J Comp Neurol.

      Volume: 印刷中 Pages: 1-14

    • DOI

      10.1002/cne.24213

    • NAID

      120006343671

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Role of mucosa in generating spontaneous activity in the guinea pig seminal vesicle2017

    • Author(s)
      Takeya Mitsue, Hikaru Hashitani, Tokumasa Hayashi, Ryuhei Higashi, Kei-ichiro Nakamura, and Makoto Takano
    • Journal Title

      The Journal of Physiology

      Volume: in press Pages: 4803-4821

    • DOI

      10.1113/jp273872

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Three-dimensional ultrastructural analyses of anterior pituitary gland expose spatial relationships between endocrine cell secretory granule localization and capillary distribution2016

    • Author(s)
      Yoshitomi M, Ohta K, Kanazawa T, Togo A, Hirashima S, Uemura K, Okayama S, Morioka M, Nakamura K
    • Journal Title

      Sci Rep.

      Volume: 6 Pages: 36019-36019

    • DOI

      10.1038/srep36019

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Histomorphometric and ultrastructural analysis of the tendon-bone interface after rotator cuff repair in a rat model2016

    • Author(s)
      Kanazawa T, Gotoh M, Ohta K, Honda H, Ohzono H, Shimokobe H, Shiba N, Nakamura K
    • Journal Title

      Scientific Reports

      Volume: 6 Pages: 33800-33800

    • DOI

      10.1038/srep33800

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Three-dimensional ultrastructural analysis of cells in the periodontal ligament using focused ion beam/scanning electron microscope tomography2016

    • Author(s)
      Hirashima S, Ohta K, Kanazawa T, Okayama S, Togo A, Uchimura N, Kusukawa J, Nakamura K
    • Journal Title

      Scientific Reports

      Volume: 6 Pages: 39435-39435

    • DOI

      10.1038/srep39435

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Chronic Fluoxetine Induces the Enlargement of Perforant Path-Granule Cell Synapses in the Mouse Dentate Gyrus.2016

    • Author(s)
      Kitahara Y, Ohta K, Hasuo H, Shuto T, Kuroiwa M, Sotogaku N, Togo A, Nakamura K, Nishi A
    • Journal Title

      PLoS ONE

      Volume: 11(1)

    • DOI

      10.1371/journal.pone.0147307

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Repeated bouts of fast eccentric contraction produce sciatic nerve damage in rats.2016

    • Author(s)
      Kouzaki K, Kobayashi M, Nakamura K, Ohta K, Nakazato K
    • Journal Title

      Muscle Nerve.

      Volume: 52 Pages: 936-942

    • DOI

      10.1002/mus.25110

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Autonomic and sensory nerve modulation of peristalsis in the upper urinary tract. Autonomic Neuroscience2016

    • Author(s)
      Nguyen MJ, Higashi R, Ohta K, Nakamura K, Hashitani H, Lang JR
    • Journal Title

      Auton Neurosci.

      Volume: *** Pages: 1-10

    • DOI

      10.1016/j.autneu.2015.07.425

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Anchoring structure of the calvarial periosteum revealed by focused ion beam/scanning electron microscope tomography2015

    • Author(s)
      Hirashima S, Ohta K, Kanazawa T, Uemura K, Togo A, Yoshitomi M, Okayama S, Kusukawa J, Nakamura K
    • Journal Title

      SCIENTIFIC REPORTS

      Volume: ***

    • DOI

      10.1038/srep17511

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Three-dimensional ultrastructureal analysis of development at the supraspinatus insertion by using focused ion beam/scanning electron microscope tomography in rats2015

    • Author(s)
      Kanazawa T, Gotoh M, Ohta K, Shiba N, Nakamura K
    • Journal Title

      J Orthop Res

      Volume: ***

    • DOI

      10.1002/jor.23111

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Three-dimensional architecture of podocytes revealed by block-face scanning electron microscopy.2015

    • Author(s)
      Ichimura K, Miyazaki N, Sadayama S, Murata K, Koike M, Nakamura K, Ohta K, Sakai T
    • Journal Title

      Sci. Rep.

      Volume: 5 Pages: 8993-8993

    • DOI

      10.1038/srep08993

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Ectopic automaticity induced in ventricular myocytes by transgenic overexpression of HCN2.2015

    • Author(s)
      Kensuke Oshita, Masayuki Itoh, Shingo Hirashima, Yoshihiro Kuwabara, Keiko Ishihara, Koichiro Kuwahara, Kazuwa Nakao, Takeshi Kimura, Kei-ichiro Nakamura, Kazuo Ushijima, Makoto Takanoa
    • Journal Title

      Journal of Molecular and Cellular Cardiology

      Volume: 80 Pages: 81-89

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Subcutaneous transplantation promotes organ formation of the fetal rat urogenital sinus2015

    • Author(s)
      Kei-ichiro Uemura, Keisuke Ohta, Tomonoshin Kanazawa, Tokumasa Hayashi, Ryuichiro Tanoue, Munetake Yoshitomi, Shingo Hirashima, Shigetaka Suekane, Kei Matsuoka, Tsukasa Igawa, Kei-ichiro Nakamura
    • Journal Title

      Acta Histochemica

      Volume: 6

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Eiichiro Tanaka, Genzou Takemura, Ken-ichiro Kosai, Yushiro Yamashita, Toyojiro Matsuishi. Disturbance of cardiac gene expression and cardiomyocyte structure predisposes Mecp2-null mice to arrhythmias2015

    • Author(s)
      Munetsugu Hara, Tomoyuki Takahashi, Chiaki Mitsumasu, Sachiyo Igata, Makoto Takano, Tomoko Minami, Hideo Yasukawa, Satoko Okayama, Keiichiro Nakamura, Yasunori Okabe
    • Journal Title

      SCIENTIFIC REPORTS

      Volume: ***

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] マウス骨格筋組織における骨髄由来細胞の分布2014

    • Author(s)
      小林正利、太田啓介、東龍平、中村桂一郎
    • Journal Title

      久留米医会誌

      Volume: 77 Pages: 34-44

    • NAID

      40020050918

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 早期口腔癌特殊光観察の臨床的意義2014

    • Author(s)
      岩本修、田上隆一郎、太田啓介、中村桂一郎、楠川仁悟
    • Journal Title

      久留米医会誌

      Volume: 77 Pages: 8-17

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] FIB/SEMトモグラフィー法による三次元再構築の特徴.2014

    • Author(s)
      太田啓介、金澤知之進、中村桂一郎
    • Journal Title

      顕微鏡

      Volume: 49 Pages: 161-165

    • NAID

      130007701724

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Efficient Transduction of 11 Poly-arginine Peptide in an Ischemic Lesion of Mouse Brain2014

    • Author(s)
      Yuki Gotanda, Fan-Yan Wei, Hideki Harada, Keisuke Ohta, Kei-ichiro Nakamura, Kazuhito Tomizawa, Kazuo Ushijima
    • Journal Title

      Journal of Stroke and Cerebrovascular Diseases

      Volume: 23 Pages: 2023-2030

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Novel characteristics of normal supraspinatus insertion in rats: an ultrastructural analysis using three-dimensional reconstruction using focused ion beam/scanning electron microscope tomography2014

    • Author(s)
      Tomonoshin Kanazawa, Masafumi Gotoh, Keisuke Ohta, Naoto Shiba, Kei-ichiro Nakamura
    • Journal Title

      Muscles, Ligaments and Tendons Journal

      Volume: 4 Pages: 182-187

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tendon-to-bone healing using autologous bone, marrow-derived mesenchymal stem cells in ACL reconstruction without a tibial bone tunnel-A histological study-.2014

    • Author(s)
      Tomonoshin Kanazawa, Takashi Soejima, Kouji Noguchi, KousukeTabuchi, Megumi Noyama, Kei-ichiro Nakamura, Naoto Shiba
    • Journal Title

      Muscles, Ligaments and Tendons Journal

      Volume: 4 Pages: 201-206

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of Silodosin and Tamsulosin on the Seminal Vesicle Contractile Response2014

    • Author(s)
      Tokumasa HAYASHI, Mitsue TAKEYA, Kei-ichiro NAKAMURA, Kei MATSUOKA
    • Journal Title

      Lower Urinary Tract Symptoms

      Volume: 7 Pages: 12072-12072

    • DOI

      10.1111/luts.12072

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Three-dimensional organization of the endoplasmic reticulum membrane around the mitochondrial constriction site in mammalian cells revealed by using focused-ion beam tomography2014

    • Author(s)
      Ohta K, Okayama S, Togo A
    • Journal Title

      Microscopy

      Volume: 63 Pages: 34-34

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Correlative light and electron microscopic observation of mitochondrial DNA in mammalian cells by using focused-ion beam scanning electron microscopy2014

    • Author(s)
      Okayama S, Ohta K, Higashi R, Nakamura K
    • Journal Title

      Microscopy

      Volume: 63 Pages: 35-35

    • Related Report
      2014 Annual Research Report
  • [Journal Article] En bloc staining with hydroquinone treatment for block face imaging2014

    • Author(s)
      Togo A, Ohta K, Higashi R, Nakamura K
    • Journal Title

      Microscopy

      Volume: 63 Pages: 36-36

    • Related Report
      2014 Annual Research Report
  • [Journal Article] 3D-ultrastructural analysis at repaired supraspinatus tendon/bone insertion in rat2014

    • Author(s)
      Kanazawa T, Gotoh M, Ohta K, Shiba N, Nakamura K
    • Journal Title

      Journal of Shoulder and Elbow Surgery

      Volume: 23 Pages: 602-602

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] ラットを用いたヒト移植脂肪組織の経時的変化に関する組織学的研究2017

    • Author(s)
      力丸由起子
    • Organizer
      第122回日本解剖学会総会・全国学術集
    • Place of Presentation
      長崎大学坂本キャンパス
    • Year and Date
      2017-03-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] 力学的負担に対する歯根膜組織変化の3次元微細構造解析2017

    • Author(s)
      平嶋 伸悟
    • Organizer
      第122回日本解剖学会総会・全国学術集会.2017.3.28~3.30.
    • Place of Presentation
      長崎大学坂本キャンパス
    • Year and Date
      2017-03-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] FIB/SEM A Novel Electron Microscope For Three-dimensional Ultrastructural Analysis2017

    • Author(s)
      中村桂一郎
    • Organizer
      The 1st ABis Symposium
    • Place of Presentation
      岡崎コンファレンスセンター
    • Year and Date
      2017-02-19
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 膀胱壁内間質細胞を中心とした周辺組織の三次元構造解析2016

    • Author(s)
      林篤正、上村慶一郎、 平嶋伸悟、武谷三恵 、 金澤知之進、太田啓介、中村桂一郎、 井川掌
    • Organizer
      第23回日本排尿機能学会
    • Place of Presentation
      東京国際フォーラム
    • Year and Date
      2016-12-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] 線維芽細胞は紡錘形か?新機軸走査型電子顕微鏡FIB/SEMによる観察2016

    • Author(s)
      中村桂一郎
    • Organizer
      第68回日本皮膚科学会西部支部学術大会
    • Place of Presentation
      米子コンベンションセンター
    • Year and Date
      2016-11-19
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] ラット骨粗鬆症モデルにおける、腱板縫合後の腱骨間治癒に関する検討2016

    • Author(s)
      金澤知之進、太田啓介、中村桂一郎
    • Organizer
      日本解剖学会第72回九州支部学術集会
    • Place of Presentation
      長崎大学医学部・良順会館
    • Year and Date
      2016-10-29
    • Related Report
      2016 Annual Research Report
  • [Presentation] 力学的負荷に対する歯根膜組織変化のの3次元微細構造解析2016

    • Author(s)
      平嶋伸悟、太田啓介、金澤知之進、都合亜記暢、内村直尚、中村桂一郎
    • Organizer
      日本解剖学会第72回九州支部学術集会
    • Place of Presentation
      長崎大学・良順会館
    • Year and Date
      2016-10-29
    • Related Report
      2016 Annual Research Report
  • [Presentation] 正常腱骨付着部と腱板縫合後腱骨間治癒の三次元超微形態解析2016

    • Author(s)
      金澤知之進、後藤昌史、大園宏城、下河邉久雄、中村桂一郎、志波直人
    • Organizer
      第43回日本肩関節学会
    • Place of Presentation
      リーガロイヤルホテル広島
    • Year and Date
      2016-10-21
    • Related Report
      2016 Annual Research Report
  • [Presentation] 正常腱骨付着部形成と腱板縫合後腱骨間治癒過程の三次元超微形態解析2016

    • Author(s)
      金澤知之進、後藤昌史、大園宏城、下河邉久雄、中村桂一郎、志波直人
    • Organizer
      第31回日本整形外科学会基礎学術集会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2016-10-13
    • Related Report
      2016 Annual Research Report
  • [Presentation] FIB/SEM tomographyを用いた尿路上皮下間質細胞の観察2016

    • Author(s)
      林篤正, 上村慶一郎, 平嶋伸悟, 金澤知之進, 武谷三恵, 太田啓介, 中村桂一郎, 井川掌
    • Organizer
      第48回日本臨床分子形態学会総会・学術集会
    • Place of Presentation
      くまもと県民交流館パレア
    • Year and Date
      2016-09-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] 正常腱骨付着部と腱板縫合後腱骨間の三次元超微形態解析2016

    • Author(s)
      金澤知之進、後藤昌史、太田啓介、志波直人、中村桂一郎
    • Organizer
      第48回日本臨床分子形態学会
    • Place of Presentation
      くまもと県民交流館パレア
    • Year and Date
      2016-09-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] 集束イオンビーム観察装置付走査型電子顕微鏡を用いた瘢痕およびケロイドの解析2016

    • Author(s)
      右田 尚, 力丸 由起子, 中村 桂一郎
    • Organizer
      第25回日本形成外科学会基礎学術集会
    • Place of Presentation
      大阪市 ナレッジキャピタル コングレ・コンベンションセンター
    • Year and Date
      2016-09-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] Three-dimensional observation of suburothelial interstitial cell by FIB/SEM tomography2016

    • Author(s)
      Hayashi T, Uemura K, Hirasima S, Kanazawa T, Takeya M, Ohta K, Nakamura K, Igawa T
    • Organizer
      46th ICS annual meeting
    • Place of Presentation
      東京国際フォーラム
    • Year and Date
      2016-09-13
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 硬組織界面における3次元微細構造解析2016

    • Author(s)
      平嶋伸悟、太田啓介、金澤知之進、都合亜記暢、岡山聡子、楠川仁悟、中村桂一郎
    • Organizer
      第58回歯科基礎医学会学術大会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2016-08-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] Three Dimensional Localization of Mitochondrial Nucleoid within a Mammalian Cell by Using CLEM Method Combined with FIB-SEM2016

    • Author(s)
      Satoko Okayama, Keisuke Ohta, Ryuhei Higashi, Kei-ichiro Nakamura
    • Organizer
      Microscopy & Microanalysis 2016 Meeting.
    • Place of Presentation
      Columbus,Ohio
    • Year and Date
      2016-07-24
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Three-dimensional organization of mitochondria-associated membrane revealed by FIB-SEM combined with live cell imaging2016

    • Author(s)
      Ohta K
    • Organizer
      11th Asia-Pacific Microscopy Conference (APMC11)
    • Place of Presentation
      Phuket
    • Year and Date
      2016-05-23
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Three-dimensional relationship between the bladder intramuscular interstitial cell and the axon innervated in the detrusor bundle by FIB/SEM tomography2016

    • Author(s)
      Hayashi T, Hirashima S, Ohta K, Nakamura K, Igawa
    • Organizer
      AUA2016
    • Place of Presentation
      San diego CA USA
    • Year and Date
      2016-05-06
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ラットを用いたヒト移植脂肪組織の経時的変化に関する組織学的研究2016

    • Author(s)
      力丸由起子、力丸英明、井野康、太田啓介、東龍平、金澤知之進、中村桂一郎、清川兼輔
    • Organizer
      第59回日本形成外科学会総会学術学会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2016-04-13
    • Related Report
      2016 Annual Research Report
  • [Presentation] FIB/SEMを用いたマウス強膜内の線維芽細胞間ネットワークの観察2016

    • Author(s)
      村田和久、都合亜記暢、太田啓介、中村桂一郎、江内田寛、平田憲
    • Organizer
      第120回日本眼科学会総会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2016-04-07
    • Related Report
      2016 Annual Research Report
  • [Presentation] 収束イオンビーム/走査電子顕微鏡複合機を用いた摘出内境界膜表面の観察2016

    • Author(s)
      平田憲、村田和久、都合亜記暢、中村桂一郎
    • Organizer
      第120回日本眼科学会総会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2016-04-07
    • Related Report
      2016 Annual Research Report
  • [Presentation] Volume CLEMによる機能構造解析への展望2016

    • Author(s)
      太田啓介、宮園佳宏、岡山聡子、金澤知之進、中村桂一郎
    • Organizer
      第121回日本解剖学会総会・全国学術集会
    • Place of Presentation
      福島
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] FIB/SEM観察による再生筋間質に現れる細胞性ネットワークの検討2016

    • Author(s)
      小林正利、太田啓介、岡山聡子、東龍平、中村桂一郎、櫻井忠義
    • Organizer
      第121回日本解剖学会 総会・全国学術集会.
    • Place of Presentation
      福島
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] FIB/SEM tomography を用いた皮膚の病態生理解析の新手法2016

    • Author(s)
      力丸由起子、太田啓介、金澤知之進、東龍平、都合亜記暢、岡山聡子、中村桂一郎
    • Organizer
      第121回日本解剖学会 総会・全国学術集会
    • Place of Presentation
      福島
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] 次世代走査型電子顕微鏡による三次元微細構造解析2016

    • Author(s)
      中村桂一郎、金澤知之進、力丸由起子、平嶋伸悟、宮園佳宏、岡山聡子、東龍平、太田啓介
    • Organizer
      第93回日本生理学会大会
    • Place of Presentation
      札幌
    • Year and Date
      2016-03-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] モルモット精嚢の自発活動発生における粘膜の役割2016

    • Author(s)
      武谷三恵、橋谷光、林篤正、中村桂一郎、鷹野誠
    • Organizer
      第93回日本生理学会大会
    • Place of Presentation
      札幌
    • Year and Date
      2016-03-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] Balamuthia mandrillaris の捕食運動2016

    • Author(s)
      原樹、東龍平、岡山聡子、太田啓介、中村桂一郎
    • Organizer
      第85回日本寄生虫学会大会
    • Place of Presentation
      宮崎
    • Year and Date
      2016-03-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] FIB/SEMを用いたミトコンドリアDNAの光顕・電顕相関観察法(CLEM)による解析2015

    • Author(s)
      岡山聡子、太田啓介、東龍平、中村桂一郎
    • Organizer
      第57回日本顕微鏡学会九州支部総会・学術講演会.
    • Place of Presentation
      福岡
    • Year and Date
      2015-11-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] ケロイドおよび瘢痕の三次元微細構造解析(正常皮膚と成熟瘢痕との比較検討)2015

    • Author(s)
      力丸由起子、太田啓介、金澤知之進、東龍平、都合亜記暢、岡山聡子、中村桂一郎
    • Organizer
      日本解剖学会第71回九州支部学術集会
    • Place of Presentation
      熊本
    • Year and Date
      2015-10-31
    • Related Report
      2015 Annual Research Report
  • [Presentation] FIB/SEMを用いたCCCP投与後のミトコンドリア超微形態解析:FIB/SEMを用いたCLEM観察2015

    • Author(s)
      宮園佳宏、太田啓介、都合亜記暢、岡山聡子、中村桂一郎
    • Organizer
      日本解剖学会第71回九州支部学術集会
    • Place of Presentation
      熊本
    • Year and Date
      2015-10-31
    • Related Report
      2015 Annual Research Report
  • [Presentation] Chronic fluoxetine treatment induces morphological changes of the perforant path synapse in the dentate gyrus.analysis of synaptic fine structures with FIB-SEM CNS-FANOS2015

    • Author(s)
      Kitahara Y, Ohta K, Shuto T, Kuroiwa M, Sotogaku N, Hasuo H, Togo A, Nakamura K
    • Organizer
      Chinese neuroscience society
    • Place of Presentation
      烏鎮 中国
    • Year and Date
      2015-09-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] FIB/SEMを用いたCLEM観察によるミトコンドリア関連小胞体の三次元構築. 臨床分子形態学会2015

    • Author(s)
      太田啓介、中村桂一郎
    • Organizer
      臨床分子形態学会
    • Place of Presentation
      長崎
    • Year and Date
      2015-09-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] ラット正常付着部と腱板縫合後腱骨間の超微形態/細胞分布の検討2015

    • Author(s)
      金澤知之進、後藤昌史、太田啓介、都合亜記暢、中村桂一郎
    • Organizer
      第47回日本臨床分子形態学会総会・学術集会
    • Place of Presentation
      長崎
    • Year and Date
      2015-09-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] FIB/SEMを用いたCLEM観察によるミトコンドリア関連小胞体の三次元構築2015

    • Author(s)
      太田啓介、中村桂一郎
    • Organizer
      第47回日本臨床分子形態学会総会・学術集会
    • Place of Presentation
      長崎
    • Year and Date
      2015-09-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] 力学的負荷に対する歯根膜組織変化の3次元超微形態解析2015

    • Author(s)
      平嶋伸悟、太田啓介、金澤知之進、都合亜記暢、楠川仁悟、中村桂一郎
    • Organizer
      第57回歯科基礎医学会学術大会・総会サテライトシンポジウム
    • Place of Presentation
      新潟
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] FIB/SEMによる下垂体三次元再構築データの定量解析の試み~下垂体前葉細胞は血管と接することで分泌顆粒を偏在させる~2015

    • Author(s)
      太田啓介、吉富宗健、中村桂一郎
    • Organizer
      第31回日本電子顕微鏡技術学会総会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-06-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] A novel stromal cell network visualized by FIB/SEM tomography2015

    • Author(s)
      Kei-ichiro Nakamura, Ryuhei Higashi, Mitsue Takeya, Tokumasa Hayashi, Shingo Hirashima, Tomonoshin Kanazawa, Michael Nguyen, Richard Lang, Hikaru Hashitani, Keisuke Ohta
    • Organizer
      Symposium “Multicellular Inputs Regulating Muscle Excitability”
    • Place of Presentation
      リノ・アメリカ合衆国
    • Year and Date
      2015-06-14 – 2015-06-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] 3D-ultrastructural analysis of the development at the supraspinatus insertion with FIB/SEM tomography2015

    • Author(s)
      金澤知之進、後藤昌史、太田啓介、都合亜記暢、中村桂一郎
    • Organizer
      日独整形外科医会
    • Place of Presentation
      神戸
    • Year and Date
      2015-05-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] SEM連続切片法による三次元再構築とCLEM観察への応用2015

    • Author(s)
      太田啓介、岡山聡子、中村桂一郎
    • Organizer
      日本顕微鏡学会第71回学術講演会
    • Place of Presentation
      京都 国立京都国際会館
    • Year and Date
      2015-05-13 – 2015-05-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] 材料上に培養した藻類のFIB/SEMを用いた形態学的評価法2015

    • Author(s)
      太田啓介、北原陽介、渡辺瑞希、忠成斗、池澤綾子、青島利裕、中村桂一郎
    • Organizer
      日本顕微鏡学会第71回学術講演会
    • Place of Presentation
      京都 国立京都国際会館
    • Year and Date
      2015-05-13 – 2015-05-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] A novel sroma cell network visualized by FIB/SEM tomography2015

    • Author(s)
      Nakamura K, Higashi R, Nguyen M, Lang R, Hirashima S, Knazawa T, Tkeya M, Hayashi T, Hashitani H, Ohta K
    • Organizer
      第120回日本解剖学会(生理学会合同)全国学術集会
    • Place of Presentation
      神戸市 神戸国際会議場
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] Analysis of synaptic connectivity with FIB/SEM: antidepressant-induced morphological changes in perforant path synapse in the dentate gyrus2015

    • Author(s)
      Kitahara Y, Ohta K, Sotogaku N, Nakamura K, Nishi A
    • Organizer
      第120回日本解剖学会(生理学会合同)全国学術集会
    • Place of Presentation
      神戸市 神戸国際会議場
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] 3D-ultrastructural analysis of the development at the supraspinatus tendon insertion with FIB/SEM tomograhy2015

    • Author(s)
      Kanazawa T, Ohta K, Hirashima S, Okayama S, Nakamura K
    • Organizer
      第120回日本解剖学会(生理学会合同)全国学術集会
    • Place of Presentation
      神戸
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] Newly characterized structure of podocytes revealed by three-dimensional analysis using block-face scanning electon microscopy2015

    • Author(s)
      Ichimura K, Miyazaki N, Sadayama S, Murata K, Koike M, Nakamura K, Ohta K, Sakai T
    • Organizer
      第120回日本解剖学会(生理学会合同)全国学術集会
    • Place of Presentation
      神戸市 神戸国際会議場
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] Epithelium-dependent periodical excitation in resopnse to stretch of guinea pig seminal vesicle2015

    • Author(s)
      Takeya M, Hayashi T, Nakamura K, Takano M
    • Organizer
      第120回日本解剖学会(生理学会合同)全国学術集会
    • Place of Presentation
      神戸市 神戸国際会議場
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] The stromal cells interaction and formed 3D network following acute muscle trauma2015

    • Author(s)
      Kobayashi M, Ohta K, Nakmaura K, Sakurai T
    • Organizer
      第120回日本解剖学会(生理学会合同)全国学術集会
    • Place of Presentation
      神戸市 神戸国際会議場
    • Year and Date
      2015-03-21 – 2015-03-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] FIB/SEM tomography による腎盤内 尿管起始部の細胞構築の観察2014

    • Author(s)
      中村 桂一郎、東 龍平、Nguyen MJ、Lang RJ、金澤 知之進、林 篤正、太田 啓介
    • Organizer
      第56回日本顕微鏡学会九州支部学術集会
    • Place of Presentation
      宮崎市 市民プラザ
    • Year and Date
      2014-12-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] Three dimensional organization of the endoplasmic reticulum membrane around the mitochondrial constriction site in mammalian cell revealed by using focused-ion beam tomography2014

    • Author(s)
      Ohta K, Okayama S, Togo A, Nakamura K
    • Organizer
      第58回日本顕微鏡学会シンポジウム
    • Place of Presentation
      福岡 九州大学医学部百年講堂
    • Year and Date
      2014-11-16 – 2014-11-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] Correlative light and electron microscopic observation of mitochondrial DNA in mammalian cells by using focused-ion beam scanning electron microscopy2014

    • Author(s)
      Okayama S, Ohta K, Higashi R, Nakamura K
    • Organizer
      第58回日本顕微鏡学会シンポジウム
    • Place of Presentation
      福岡 九州大学医学部百年講堂
    • Year and Date
      2014-11-16 – 2014-11-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] en bloc staining with HQ treatment for the block face imaging2014

    • Author(s)
      Togo A, Ohta K, Higashi R, Nakamura K
    • Organizer
      第58回日本顕微鏡学会シンポジウム
    • Place of Presentation
      福岡 九州大学医学部百年講堂
    • Year and Date
      2014-11-16 – 2014-11-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] ラットを用いた移植肋軟骨の経時的変化に関する組織学的研究2014

    • Author(s)
      右田尚、力丸由起子、力丸英明、太田啓介、清川兼輔、中村桂一郎
    • Organizer
      第70回日本解剖学会九州支部学術集会
    • Place of Presentation
      北九州市 産業医科大学ラマツィーニホール
    • Year and Date
      2014-10-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] 出生後における正常腱骨付着部形成過程の超微形態構造解析―肩関節腱板縫合後の組織変化との比較―2014

    • Author(s)
      金澤知之進,中村桂一郎他
    • Organizer
      第46回日本臨床分子形態学会総会・学術集会
    • Place of Presentation
      東京都 TKP市ヶ谷カンファレンスセンター
    • Year and Date
      2014-10-17 – 2014-10-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] Detailed membrane association between mitochondria and surrounding endoplasmic reticulum in mammal cells revealed by three-dimensional reconstruction using focused ion beam scanning electron microscope2014

    • Author(s)
      Ohta K, Okayama S, Togo A, Nakamura K
    • Organizer
      18th International Microscopy Congress
    • Place of Presentation
      プラハ・チェコ
    • Year and Date
      2014-09-07 – 2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] Three-dimensional distribution of the mitochondrial DNA in the mammalian cell using FIB/SEM2014

    • Author(s)
      Okayama S, Ohta K, Higashi R, Nakamura K
    • Organizer
      18th International Microscopy Congress
    • Place of Presentation
      プラハ・チェコ
    • Year and Date
      2014-09-07 – 2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] Effect of hydroquinone treatment on OTO en bloc stained biological specimens2014

    • Author(s)
      Togo A, Higashi R, Ohta K, Nakamura K
    • Organizer
      18th International Microscopy Congress
    • Place of Presentation
      プラハ・チェコ
    • Year and Date
      2014-09-07 – 2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] SEM連続断面観察法におけるFIB/SEMトモグラフィー法の特徴2014

    • Author(s)
      太田啓介、中村桂一郎
    • Organizer
      第70回日本顕微鏡学会記念学術集会
    • Place of Presentation
      千葉市 幕張メッセ国際会議場
    • Year and Date
      2014-05-10 – 2014-05-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] SEM断面観察用en bloc染色におけるHydroquinon処理の効果について2014

    • Author(s)
      都合亜紀暢、東龍平、太田啓介、中村桂一郎
    • Organizer
      第70回日本顕微鏡学会記念学術集会
    • Place of Presentation
      千葉市 幕張メッセ国際会議場
    • Year and Date
      2014-05-10 – 2014-05-13
    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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