Circadian transcriptome analysis for protection against obesity development

• Project/Area Number: 26293048
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Environmental physiology(including physical medicine and nutritional physiology)
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Koike Nobuya 京都府立医科大学, 医学(系)研究科(研究院), 講師 (00399685)
• Research Collaborator: Chen Zheng The University of Texas Health Science Center at Houston, Department of Biochemistry & Molecular Biology, Associate Professor
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000); Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2015: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2014: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
• Keywords: 概日リズム / トランスクリプトーム / メタボリズム / 時計遺伝子 / 体内時計 / 制限給餌 / 生理学 / エピゲノム

Outline of Final Research Achievements

In mammals, the circadian clock is cell autonomous and comprised of an autoregulatory transcriptional and translational feedback loop composed of several clock genes and their protein products. A growing body of evidence revealed a close link between the circadian clock and metabolism. In this study, using a mouse model of diet-induced obesity, we revealed that enhancement of circadian clock oscillation renders protection against metabolic syndrome. Daily restricted feeding caused disturbance of circadian transcriptional networks in a tissue specific manner. The genes involved in lipid metabolism were enriched in the differentially expressed genes between daytime and nighttime food restriction, suggesting that they may contribute to obesity.

Research Products

• [Int'l Joint Research] テキサス大学(米国)
• [Journal Article] The Small Molecule Nobiletin Targets the Molecular Oscillator to Enhance Circadian Rhythms and Protect against Metabolic Syndrome (2016)
  • Author(s): Baokun He, Kazunari Nohara, Noheon Park, Yong-Sung Park, Bobby Guillory, Zhaoyang Zhao, Jose M. Garcia, Nobuya Koike, Cheng Chi Lee, Joseph S. Takahashi, Seung-Hee Yoo, Zheng Chen
  • Journal Title: Cell Metab Volume: 23 Issue: 4 Pages: 610-621
  • DOI: 10.1016/j.cmet.2016.03.007
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Dual attenuation of proteasomal and autophagic BMAL1 degradation in ClockΔ19/+ mice contributes to improved glucose homeostasis (2015)
  • Author(s): Kwon Jeong, Baokun He, Kazunari Nohara, Noheon Park, Youngmin Shin, Seonghwa Kim, Kazuhiro Shimomura, Nobuya Koike, Seung-Hee Yoo, and Zheng Chen
  • Journal Title: Sci Rep Volume: 5 Issue: 1 Pages: 12801-12801
  • DOI: 10.1038/srep12801
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Ammonia-lowering activities and carbamoyl phosphate synthetase 1 (Cps1) induction mechanism of a natural flavonoid (2015)
  • Author(s): Kazunari Nohara, Youngmin Shin, Noheon Park, Kwon Jeong, Baokun He, Nobuya Koike, Seung-Hee Yoo, and Zheng Chen
  • Journal Title: Nutr Metab (Lond) Volume: 12 Issue: 1 Pages: 23-23
  • DOI: 10.1186/s12986-015-0020-7
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 概日転写サイクルとクロマチンランドスケープ (2015)
  • Author(s): 小池宣也
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-04
  • Invited