| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2016: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
|
|---|
| Outline of Final Research Achievements |
Autophagy is a major intracellular degradation system that is strongly induced upon nutrient starvation, but its physiological importance is not well understood. In this study, we tested the possibility that autophagy provides amino acids to produce proteins required for starvation adaptation in vivo. We found that protein synthesis is indeed impaired in autophagy-deficient livers during starvation. Proteomic analysis of livers from wild-type and autophagy-defective liver under normal and starvation conditions revealed that autophagy deficiency caused more drastic changes in proteome profiles than starvation. Furthermore, the intracellular amino acids levels were not significantly changed in autophagy-deficient livers even under starvation condition. These data suggest that translation attenuation in autophagy-deficient livers is caused not due to a lack of amino acids provided by autophagy but to a defect in liver homeostasis.
|
