| Budget Amount *help |
¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2016: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
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| Outline of Final Research Achievements |
In this project, we investigated the involvement of p57 gene, one of the Cyclin-dependent kinase inhibitors (CDKIs), in the regulation of self-renewal and maintenance of spermatogonial stem cells (SSCs). The results showed that the expression of p57 gene was upregulated in SSCs by FGF2 and GDNF, the self-renewal factors of SSCs. Using cultured SSC cell lines, which were derived from conditional knockout mice of p57 gene, we identified that self-renewal activity of SSCs was reduced in vitro by p57 gene deficiency. To elucidate the signaling pathway, which mediates the effect of p57 gene, we screened for molecules by microarray analysis, whose expression levels were affected by p57 gene deficiency. Involvements of some of these molecules were confirmed by overexpression or knockdown in cultured SSC cell line.
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