Structural study on the role of post-translational lipid modifications of the small G protein Ras
| Project/Area Number |
26293065
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | Kobe University |
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Principal Investigator |
KATAOKA TOHRU 神戸大学, 大学院医学研究科, 教授 (40144472)
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| Co-Investigator(Kenkyū-buntansha) |
島 扶美 神戸大学, 医学(系)研究科(研究院), 教授 (60335445)
枝松 裕紀 神戸大学, 医学(系)研究科(研究院), 助教 (70335438)
松本 篤幸 神戸大学, 医学(系)研究科(研究院), 助教 (00753906)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2016: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2015: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
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| Keywords | シグナル伝達 / 蛋白質 / 癌 / 脂質 / 高分子構造・物性 |
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| Outline of Final Research Achievements |
We succeeded in large scale preparation and purification of the post-translationally lipid-modified form of H-Ras protein and various intermediates of the modification by utilizing the protein-ligation reaction catalyzed by Sortase A. Structural analyses of the GTP-bound form of post-translationally-modified H-Ras using nuclear magnetic resonance spectroscopy and X-ray crystallography revealed that the modified C terminus established an intramolecular interaction with the N-terminal catalytic domain of H-Ras and positioned the post-translationally-attached farnesyl moiety at the proximity of the activator region, thereby forming a new binding interface for a Ras effector protein, c-Raf-1.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] Molecular Mechanism for Conformational Dynamics of Ras·GTP Elucidated from In-Situ Structural Transition in Crystal.2016
Author(s)
Matsumoto S, Miyano N, Baba S, Liao J, Kawamura T, Tsuda C, Takeda A, Yamamoto M, Kumasaka T, Kataoka T, Shima F.
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Journal Title
Sci Rep.
Volume: -
Issue: 1
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Deletion of Rapgef6, a candidate schizophrenia susceptibility gene, disrupts amygdala function in mice.2015
Author(s)
R. J. Levy, M. Kvajo, Y. Li, E. Tsvetkov, W. Dong, Yoko Yoshikawa, Tohru Kataoka, V. Y. Bolshakov, M. Karayiorgou, J. A. Gogos
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Journal Title
Translational Psychiatry
Volume: 5
Issue: 6
Pages: e577-e577
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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