Elucidation of chemoresistance of refracory cancer by single-cell gene expression analyses
| Project/Area Number |
26293073
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | National Cancer Center Japan |
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Principal Investigator |
Okamoto Koji 国立研究開発法人国立がん研究センター, その他部局等, その他 (80342913)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAGAMA Hitoshi 国立がん研究センター, 理事長 (30198030)
KATO Mamoru 国立がん研究センター研究所, 基盤的臨床開発研究コアセンター・バイオインフォマティクス部門, 部門長 (40391916)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
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| Keywords | 癌 / 分子腫瘍学 |
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| Outline of Final Research Achievements |
It is likely that cancer chemoresistance is attributed to a subset of chemoresistant cells in cancer tissues. Therefore, it will be important to understand cancer heterogeneity to overcome cancer chemorisitance and cure refractory cancer. We aimed to elucidate cancer heterogeneity by examining mouse xenograft tumors derived from in-vitro cultivated human colon cancer stem cells. By performing single-cell gene expression analyses of the tumors, we demonstrated that a subset of Lgr5-positive stem-like cells are associated with chemoresitance. We also performed single-cell analyses in a mouse model of colon carcinogenesis and identified a stem cell group that is responsible for tumorigenicity of colon tumors.
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] Sox2-dependent inhibition of p21 is associated with poor prognosis of endometrial cancer.2017
Author(s)
Yamawaki K, Ishiguro T, Mori Y, Yoshihara K, Suda K, Tamura R, Yamaguchi M, Sekine M, Kashima K, Higuchi M, Fujii M, Okamoto K, Enomoto T
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Journal Title
Cancer Sci.
Volume: 108
Issue: 4
Pages: 632-640
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] TNIK inhibition abrogates colorectal cancer stemness.2016
Author(s)
Masuda M, Uno Y, Ohbayashi N, Ohata H, Mimata A, Kukimoto-Niino M, Moriyama H, Kashimoto S, Inoue T, Goto N, Okamoto K, Shirouzu M, Sawa M, Yamada T.
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Journal Title
Nature Comm.
Volume: -
Issue: 1
Pages: 12586-12586
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Establishment and characterization of an in vitro model of ovarian cancer stem-like cells with an enhanced proliferative capacity.2016
Author(s)
Ishiguro T, Sato A, Ohata H, Ikarashi Y, Takahashi R, Ochiya T, Yoshida M, Tsuda H, Onda T, Kato T, Kasamatsu T, Enomoto T, Tanaka K, Nakagama H, Okamoto K.
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Journal Title
Cancer Research
Volume: 76
Issue: 1
Pages: 150-160
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] MicroRNA-574-3p, identified by microRNA library-based functional screening, modulates tamoxifen response in breast cancer2015
Author(s)
Ujihira T, Ikeda K, Suzuki T, Yamaga R, Sato W, Horie-Inoue K, Shigekawa T, Osaki A, Saeki T, Okamoto K, Takeda S, Inoue S
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Journal Title
Sci Rep
Volume: 5
Issue: 1
Pages: 7641-7641
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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