Development of miRNA replacement therapy

• Project/Area Number: 26293088
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Tokyo Medical University
• Principal Investigator: Masahiko Kuroda 東京医科大学, 医学部, 教授 (80251304)
• Co-Investigator(Kenkyū-buntansha): 村上 善基 大阪市立大学, 医学(系)研究科(研究院), 准教授 (00397556) ; 須藤 カツ子 東京医科大学, 医学部, 兼任講師 (50126091)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000); Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
• Keywords: miRNA / miR-34 / miR-29 / IPF / Dicer / Ago / 非小細胞性肺がん / ドラッグデリバリーシステム / 肺がん / 肺腺癌 / miR34 / miR29

Outline of Final Research Achievements

MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression and fundamental cell processes such as development, differentiation, proliferation, and apoptosis. Reduced or elevated expression of miRNAs has been implicated in the development of various human diseases, including cancer. Therefore, miRNA replacement therapy represents a promising approach for the treatment of lung cancer. In this project, we developed a 30-nucleotide single-strand RNA, termed “guide hairpin RNA (ghRNA, ghR)”, that has a physiological function similar to miRNA. The ghR caused no innate cytokine response either in vitro or in vivo. In addition, systemic and local injection of ghR-form miR-34a (ghR-34a) suppressed tumor growth in a mouse model of RAS-induced lung cancer. This novel RNA interference (RNAi) technology may provide a novel, safe, and effective nucleic acid drug platform that will increase the clinical usefulness of nucleic acid therapy.

Research Products

• [Journal Article] Vascular Smooth Muscle Cell Maturation Stage and Ki-67 Index are Diagnostic Biomarkers for Pathologic Grade of Ovarian Teratoma. (2017)
  • Author(s): Hongo-Kohama M, Kurata A, Hashimoto H, Fujita K, Horiuchi H, Nagao T, Kuroda M.
  • Journal Title: Int J Gynecol Pathol. Volume: ahead of print Issue: 6 Pages: 582-592
  • DOI: 10.1097/pgp.0000000000000373
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Repression of Smad3 by Stat3 and c-Ski/SnoN induces gefitinib resistance in lung adenocarcinoma. (2017)
  • Author(s): Makino Y, Yoon JH, Bae E, Kato M, Miyazawa K, Ohira T, Ikeda N, Kuroda M, Mamura M.
  • Journal Title: Biochem Biophys Res Commun. Volume: 484 Issue: 2 Pages: 269-77
  • DOI: 10.1016/j.bbrc.2017.01.093
  • NAID: 120007134941
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Novel form of miR-29b suppresses bleomycin-induced pulmonary fibrosis. (2017)
  • Author(s): Yamada Y, Takanashi M, Sudo K, Ueda S, Ohno SI, Kuroda M.
  • Journal Title: PLoS One. Volume: 12 Issue: 2 Pages: 0171957-0171957
  • DOI: 10.1371/journal.pone.0171957
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cardiac myofibroblast engulfment of dead cells facilitates recovery after myocardial infarction. (2017)
  • Author(s): Michio Nakaya,Kenji Watari,Mitsuru Tajima,Takeo Nakaya,Shoichi Matsuda,Hiroki Ohara,Hiroaki Nishihara,Hiroshi Yamaguchi,Akiko Hashimoto,Mitsuho Nishida,Akiomi Nagasaka,Yuma Horii,Hiroki Ono,Gentaro Iribe,Ryuji Inoue,Makoto Tsuda,Kazuhide Inoue,Akira Tanaka,Masahiko Kuroda,Shigekazu Nagata,Hitoshi Kurose
  • Journal Title: Journal of Clinical Investigation Volume: 127 Issue: 1 Pages: 383-401
  • DOI: 10.1172/jci83822
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] MiR-29a Assists in Preventing the Activation of Human Stellate Cells and Promotes Recovery From Liver Fibrosis in Mice. (2016)
  • Author(s): Matsumoto Y, Itami S, Kuroda M, Yoshizato K, Kawada N, Murakami Y.
  • Journal Title: Mol Ther. Volume: 24 Issue: 10 Pages: 1848-59
  • DOI: 10.1038/mt.2016.127
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Necrotizing arteritis occurring in an intralobar pulmonary sequestration of a patient without systemic vasculitis syndrome. (2016)
  • Author(s): Hashimoto H, Hara K, Matsumoto J, Nashiro T, Nagano M, Kusakabe M, Kurata A, Kuroda M, Suzuki Y, Horiuchi H.
  • Journal Title: Cardiovasc Pathol. Volume: 25 Issue: 3 Pages: 200-2
  • DOI: 10.1016/j.carpath.2016.01.005
  • Peer Reviewed / Open Access
• [Journal Article] Development of novel small hairpin RNAs that do not require processing by dicer or AGO2 (2016)
  • Author(s): Ohno S, Itano K, Harada Y, Asada K, Oikawa K, Kashiwazako M, Okuyama H, Kumagai K, Takanashi M, Sudo K, Ikeda N, Kuroda M.
  • Journal Title: Mol Ther Volume: 24 Issue: 7 Pages: 1278-1289
  • DOI: 10.1038/mt.2016.81
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] HLA-G expression is regulated by miR-365 in trophoblasts under hypoxic conditions. (2016)
  • Author(s): Mori A, Nishi H, Sasaki T, Nagamitsu Y, Kawaguchi R, Okamoto A, Kuroda M, Isaka K.
  • Journal Title: Placenta. Volume: 45 Pages: 37-41
  • DOI: 10.1016/j.placenta.2016.07.004
  • Peer Reviewed / Open Access
• [Journal Article] Cord Blood-Derived Endothelial Colony-Forming Cell Function is Disrupted in Congenital Diaphragmatic Hernia (2016)
  • Author(s): Hideshi Fujinaga, Hiroko Fujinaga, Nobuyuki Watanabe, Tomoko Kato, Moe Tamano, Miho Terao, Shuji Takada, Yushi Ito, Akihiro Umezawa, Masahiko Kuroda
  • Journal Title: American Journal of Physiology-Lung Cellular and Molecular Physiology Volume: 310 Issue: 11 Pages: 1143-1154
  • DOI: 10.1152/ajplung.00357.2015
  • NAID: 120005845870
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Solitary Pulmonary Capillary Hemangioma of Adult Cases: Clinicopathologic Characteristics as an Unrecognized Entity. (2016)
  • Author(s): Hashimoto H, Kurata A, Fujiwara M, Hara K, Matsumoto J, Kusakabe M, Tachibana K, Nashiro T, Terado Y, Akahane M, Usui K, Suzuki Y, Kondo H, Kamma H, Kuroda M, Horiuchi H.
  • Journal Title: Am J Surg Pathol. Volume: 40 Issue: 10 Pages: 1380-1389
  • DOI: 10.1097/pas.0000000000000708
  • Peer Reviewed / Open Access
• [Journal Article] Topical Use of Angiopoietin-like Protein 2 RNAi-loaded Lipid Nanoparticles Suppresses Corneal Neovascularization. (2016)
  • Author(s): Taketani Y, Usui T, Toyono T, Shima N, Yokoo S, Kimakura M, Yamagami S, Ohno S, Onodera R, Tahara K, Takeuchi H, Kuroda M.
  • Journal Title: Mol Ther Nucleic Acids. Volume: 5 Pages: e292-e292
  • DOI: 10.1038/mtna.2016.1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] MicroRNA and gynecologic cancers. (2016)
  • Author(s): Kanekura K, Nishi H, Isaka K, Kuroda M.
  • Journal Title: J Obstet Gynaecol Res. Volume: 10.1111/jog.12995 Issue: 6 Pages: 052-052
  • DOI: 10.1111/jog.12995
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Poly-dipeptides encoded by the C9ORF72 repeats block global protein translation. (2016)
  • Author(s): Kanekura K, Yagi T, Cammack AJ, Mahadevan J, Kuroda M, Harms MB, Miller TM, Urano F.
  • Journal Title: Hum Mol Genet. Volume: ahead of print
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Focus on Extracellular Vesicles: Development of Extracellular Vesicle-Based Therapeutic Systems. (2016)
  • Author(s): Ohno S, Drummen G, Kuroda M.
  • Journal Title: Int. J. Mol. Sci. Volume: 17(2) Issue: 2 Pages: 172-191
  • DOI: 10.3390/ijms17020172
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Quantitative nucleic features are effective for discrimination of intraductal proliferative lesions of the breast. (2016)
  • Author(s): Yamada M, Saito A, Yamamoto Y, Cosatto E, Kurata A, Nagao T, Tateishi A, Kuroda M.
  • Journal Title: J Pathol Inform Volume: 7 Issue: 1 Pages: 7-7
  • DOI: 10.4103/2153-3539.175380
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Novel types of small RNA exhibit sequence- and target-dependent angiogenesis suppression without activation of Toll-like Receptor 3 in an age-related macular degeneration (AMD) mouse model. (2015)
  • Author(s): Takanashi M, Sudo K, Ueda S, Ohno S, Yamada Y, Osakabe Y, Goto H, Matsunaga Y, Ishikawa A, Usui , Kuroda M.
  • Journal Title: Mol Ther Nucleic Acids. Volume: 4 Pages: e258-e258
  • DOI: 10.1038/mtna.2015.34
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Does perineural invasion of the myenteric plexus have a key role in annular rectal invasion and digestive system symptoms of prostate carcinoma patients? (2015)
  • Author(s): Hashimoto H, Kurata A, Nashiro T, Kuroda M, Horiuchi H
  • Journal Title: Med Hypotheses Volume: 85 Issue: 6 Pages: 854-7
  • DOI: 10.1016/j.mehy.2015.09.024
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Phosphorylation status determines the opposing functions of Smad2/Smad3 as STAT3 cofactors in TH17 differentiation. (2015)
  • Author(s): Yoon JH, Sudo K, Kuroda M, Kato M, Lee IK, Han JS, Nakae S, Imamura T, Kim J, Ju JH, Kim DK, Matsuzaki K, Weinstein M, Matsumoto I, Sumida T, Mamura M
  • Journal Title: Nat Commun. Volume: 6 Issue: 1 Pages: 7600-7600
  • DOI: 10.1038/ncomms8600
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Ovarian small cell carcinoma of pulmonary type appearing in ante-mortem ascites: An autopsy case and review of the literature (2015)
  • Author(s): Hirotsugu Hashimoto, Atsushi Kurata, Koji Fujita, Hideto Shimada, Takeshi Nagai, Hajime Horiuchi, Masahiko Kuroda
  • Journal Title: Human Pathology: Case Reports Volume: 未定 Issue: 3 Pages: 6-6
  • DOI: 10.1016/j.ehpc.2015.02.003
  • Peer Reviewed / Open Access
• [Journal Article] Angiopoietin-like 7 is an anti-angiogenic protein required to prevent vascularization of the cornea. (2015)
  • Author(s): Toyono T, Usui T, Yokoo S, Taketani Y, Nakagawa S, Kuroda M, Yamagami S, Amano S.
  • Journal Title: PLoS One Volume: 10 Issue: 1 Pages: e0116838-e0116838
  • DOI: 10.1371/journal.pone.0116838
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Inhibition of choroidal fibrovascular membrane formation by new class of RNA interference therapeutic agent targeting periostin. (2015)
  • Author(s): Nakama T, Yoshida S, Ishikawa K, Kobayashi Y, Zhou Y, Nakao S, Sassa Y, Oshima Y, Takao K, Shimahara A, Yoshikawa K, Hamasaki T, Ohgi T, Hayashi H, Matsuda A, Kudo A, Nozaki M, Ogura Y, Kuroda M, Ishibashi T.
  • Journal Title: Gene Therapy. Volume: 22 Issue: 2 Pages: 127-137
  • DOI: 10.1038/gt.2014.112
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Immaturity of smooth muscle cells in the neointima is associated with acute coronary syndrome. (2015)
  • Author(s): Horita A, Kurata A, Ohno S, Shimoyamada H, Saito I, Kamma H, Kuroda M.
  • Journal Title: Cardiovasc Pathol Volume: 24 Issue: 1 Pages: 26-32
  • DOI: 10.1016/j.carpath.2014.09.003
  • Peer Reviewed / Open Access
• [Journal Article] Evaluation of the Human Papillomavirus mRNA Test for the detection of cervical lesions in Japan. (2015)
  • Author(s): Nakayama Y, Yamada M, Kurata A, Kiseki H, Kuroda M.
  • Journal Title: Eur J Gynaec Oncol Volume: in press
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Unique expression pattern of viral proteins in human herpesvirus 8-positive plasmablastic lymphoma: a case report. (2014)
  • Author(s): Yamada M, Katano H, Yotsumoto M, Hashimoto H, Muramatsu T, Shiotsuka M, Fukutake K, Kuroda M.
  • Journal Title: Int J Clin Exp Pathol Volume: 15 Pages: 6415-6418
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] In vivo challenging of polymyxins and levofloxacin eye drop against multidrug-resistant Pseudomonas aeruginosa keratitis. (2014)
  • Author(s): Tajima K, Miyake T, Koike N, Hattori T, Kumakura S, Yamaguchi T, Matsumoto T, Fujita K, Kuroda M, Ito N, Goto H.
  • Journal Title: J Infect Chemother. Volume: 20 Issue: 6 Pages: 343-349
  • DOI: 10.1016/j.jiac.2013.10.015
  • Peer Reviewed
• [Journal Article] Multifocal Kaposiform Hemangioendothelioma in Multiple Visceral Organs: An Autopsy of 9-Day-Old Female Baby. (2014)
  • Author(s): Takeo Nakaya, Kiyoko Morita, Atsushi Kurata,Tetsuo Ushiku, Takashi Igarashi, Masahiko Kuroda, Masashi Fukayama.
  • Journal Title: Human Pathology. Volume: 45 Issue: 8 Pages: 1773-1777
  • DOI: 10.1016/j.humpath.2014.03.019
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] KLF5 Regulates the Integrity and Oncogenicity of Intestinal Stem Cells (2014)
  • Author(s): Nakaya, T
  • Journal Title: Cancer Res Volume: 74 Issue: 10 Pages: 2882-2891
  • DOI: 10.1158/0008-5472.can-13-2574
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Young-age-onset pancreatoduodenal carcinoma in Shwachman-Diamond syndrome. (2014)
  • Author(s): Nakaya T, Kurata A, Hashimoto H, Nishimata S, Kashiwagi Y, Fujita K, Kawashima H, Kuroda M.
  • Journal Title: Pathol Int Volume: 64 Issue: 2 Pages: 75-80
  • DOI: 10.1111/pin.12133
  • Peer Reviewed
• [Presentation] Digital Pethologyを用いた肝細胞がんの形態学解析による再発予測の検討. (2017)
  • Author(s): 齋藤 彰、藤田浩司、山本陽一郎、硲 彰一、坂本和彦、永野浩昭、黒田雅彦.
  • Organizer: 第106回日本病理学会総会
  • Place of Presentation: 京王プラザホテル(東京)
  • Year and Date: 2017-04-27
• [Presentation] 子宮頸部上皮内腫瘍病変に対する新規モノクロナール抗体の開発. (2017)
  • Author(s): 藤田浩司、大野慎一郎、西 洋孝、井坂恵一、黒田雅彦.
  • Organizer: 第106回日本病理学会総会
  • Place of Presentation: 京王プラザホテル(東京)
  • Year and Date: 2017-04-27
• [Presentation] Activation of TLR2 regulates CARD10 gene expression by miR-146a-5p in oral squamous cell carcinoma. (2017)
  • Author(s): 池畑直樹、高梨正勝、上田しのぶ、里見貴史、渡辺正人、長谷川温、河野通秀、榎本 愛、黒田雅彦、近津大地.
  • Organizer: 第106回日本病理学会総会
  • Place of Presentation: 京王プラザホテル(東京)
  • Year and Date: 2017-04-27
• [Presentation] RNA干渉核酸に働く短鎖ヘアピンRNAの探索と機能解析 (2017)
  • Author(s): 大野慎一郎、原田裕一郎、村上善基、黒田雅彦.
  • Organizer: 第106回日本病理学会総会
  • Place of Presentation: 京王プラザホテル(東京)
  • Year and Date: 2017-04-27
• [Presentation] miRNA生成経路を欠損する肺がん細胞株の解析. (2017)
  • Author(s): 老川桂生、淺田浩太朗、大野慎一郎、黒田雅彦.
  • Organizer: 第106回日本病理学会総会
  • Place of Presentation: 京王プラザホテル(東京)
  • Year and Date: 2017-04-27
• [Presentation] Clinicopathogic Characteristics of solitary pulmonary capillary hemangioma. (2017)
  • Author(s): 橋本浩次、倉田 厚、藤原正親、原 啓、名城珠希、増田芳雄、鈴木良夫、菅間 博、黒田雅彦、堀内 啓.
  • Organizer: 第106回日本病理学会総会
  • Place of Presentation: 京王プラザホテル(東京)
  • Year and Date: 2017-04-27
• [Presentation] 浸潤性乳管癌に出現するlet-7発現細胞の検討. (2017)
  • Author(s): 鶴井敏光、藤田浩司、大野慎一郎、黒田雅彦.
  • Organizer: 第106回日本病理学会総会
  • Place of Presentation: 京王プラザホテル(東京)
  • Year and Date: 2017-04-27
• [Presentation] Mir-34aによるがん抑制遺伝子BLUの発現制御機構. (2017)
  • Author(s): 三部芳裕、大野慎一郎、黒田雅彦.
  • Organizer: 第106回日本病理学会総会
  • Place of Presentation: 京王プラザホテル(東京)
  • Year and Date: 2017-04-27
• [Presentation] RNAワールドと肺がん発がん機構 (2016)
  • Author(s): 黒田雅彦.
  • Organizer: 第108回ACCP日本部会定期教育講演会.
  • Place of Presentation: 東京医科大学(東京)
  • Year and Date: 2016-10-29
  • Invited
• [Presentation] TLS-CHOPを発現する粘液型脂肪肉腫細胞における腫瘍関連分子経路の解析. (2016)
  • Author(s): 及川恒輔、高梨正勝、佐野冬樹、黒田雅彦、村垣泰光.
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2016-10-06
• [Presentation] 新規ヘアピン型RNA干渉核酸の探索と機能解析. (2016)
  • Author(s): 大野慎一郎、原田裕一郎、村上善基、黒田雅彦.
  • Organizer: 第8回日本RNAi研究会、第3回日本細胞外小胞体学会
  • Place of Presentation: グランドプリンスホテル広島(広島)
  • Year and Date: 2016-08-31
• [Presentation] miRNA生成経路を欠損する肺がん細胞株の解析 (2016)
  • Author(s): 老川桂生、浅田浩太朗、大野慎一郎、黒田雅彦
  • Organizer: 第105回日本病理学会総会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2016-05-12
• [Presentation] 肺がんにおけるArgonauteファミリーの機能解析 (2016)
  • Author(s): 大野慎一郎、浅田浩太朗、老川桂生、原田裕一郎、黒田雅彦
  • Organizer: 第105回日本病理学会総会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2016-05-12
• [Presentation] 乳がん幹細胞の酸化ストレス抵抗性におけるmiR-27aの作用 (2016)
  • Author(s): 上田しのぶ、高梨正勝、黒田雅彦
  • Organizer: 第105回日本病理学会総会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2016-05-12
• [Presentation] 血管炎症候群を伴わない患者の肺葉内肺分画症に見られた壊死性動脈炎 (2016)
  • Author(s): 橋本浩次、原 啓、松本 順、名城珠希、増田芳雄、長野匡晃、日下部将司、黒田雅彦、鈴木良夫、堀内 啓
  • Organizer: 第105回日本病理学会総会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2016-05-12
• [Presentation] Development of novel Dicer- and Ago2-independent small hairpin RNAs (2016)
  • Author(s): Shin-ichiro OHNO, Karen ITANO, Yuicirou HARADA, Koutaro ASADA, Keiki OIKAWA, Mikie KASHIWAZAKO, Hikaru OKUYAMA, Katsuyoshi KUMAGAI, Masakatsu TAKANASHI, Katsuko SUDO, Norihiko IKEDA, Masahiko KURODA.
  • Organizer: American Society of Gene & Cell Therapy(ASGCT) 19th Annual Meeting. (第19回米国遺伝子治療学会)
  • Place of Presentation: Marriot Wardman Park Hotel, Washington,DC, USA
  • Year and Date: 2016-05-04
  • Int'l Joint Research
• [Presentation] エクソソームが開く新しい医療 (2015)
  • Author(s): 黒田雅彦
  • Organizer: 第19回日本統合医療学会
  • Place of Presentation: 山口市民会館（山口県山口市）
  • Year and Date: 2015-12-12
  • Invited
• [Presentation] 子宮頸部上皮腫瘍（cervical intraepithelial neoplasia; CIN）の抗MUC1抗体における染色性の検討 (2015)
  • Author(s): 藤田浩司、倉田 厚、別所泰子、峯尾松一郎、黒田雅彦
  • Organizer: 第12回日本病理学会カンファレンス
  • Place of Presentation: 六甲山ホテル（神戸市灘区）
  • Year and Date: 2015-07-24
• [Presentation] ダニ抗原で誘発させたアトピー性皮膚炎モデルマウスの作成 (2015)
  • Author(s): 柏迫幹絵、高梨正勝、黒田雅彦
  • Organizer: 第12回日本病理学会カンファレンス
  • Place of Presentation: 六甲山ホテル（神戸市灘区）
  • Year and Date: 2015-07-24
• [Presentation] 肺線維症に対するmiR-29b補充療法 (2015)
  • Author(s): 山田侑子、高梨正勝、須藤カツ子、上田しのぶ、黒田雅彦
  • Organizer: 第12回日本病理学会カンファレンス
  • Place of Presentation: 六甲山ホテル（神戸市灘区）
  • Year and Date: 2015-07-24
• [Presentation] 肺がんにおけるArgonauteﾌｧﾐﾘｰの発現・機能解析 (2015)
  • Author(s): 浅田浩太朗、老川桂生、大野慎一郎、黒田雅彦
  • Organizer: 第12回日本病理学会カンファレンス
  • Place of Presentation: 六甲山ホテル（神戸市灘区）
  • Year and Date: 2015-07-24
• [Presentation] microRNAの生成経路を欠損する肺がん細胞株の解析 (2015)
  • Author(s): 老川桂生、浅田浩太朗、大野慎一郎、黒田雅彦
  • Organizer: 第12回日本病理学会カンファレンス
  • Place of Presentation: 六甲山ホテル（神戸市灘区）
  • Year and Date: 2015-07-24
• [Presentation] 副作用を軽減した短鎖型核酸医薬の開発 (2015)
  • Author(s): 大野慎一郎、板野華蓮、浅田浩太朗、老川桂生、柏迫幹絵、高梨正勝、黒田雅彦
  • Organizer: 第12回日本病理学会カンファレンス
  • Place of Presentation: 六甲山ホテル（神戸市灘区）
  • Year and Date: 2015-07-24
• [Presentation] 計測画像情報の病理学への応用 (2015)
  • Author(s): 黒田雅彦
  • Organizer: 第12回日本病理学会カンファレンス
  • Place of Presentation: 六甲山ホテル（神戸市灘区）
  • Year and Date: 2015-07-24
  • Invited
• [Presentation] 浸潤性乳癌に出現するlet-7d発現細胞の検討． (2015)
  • Author(s): 藤田浩司、山田正俊、高梨正勝、黒田雅彦．
  • Organizer: 第104回日本病理学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-04-30 – 2015-05-02
• [Presentation] 肺線維症に対するmiR-29b 補充療法． (2015)
  • Author(s): 山田侑子、高梨正勝、須藤カツ子、上田しのぶ、黒田雅彦．
  • Organizer: 第104回日本病理学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-04-30 – 2015-05-02
• [Presentation] 胃癌細胞株の形態変化における、上皮間葉系移行に関わるマイクロRNA200cとその標的の検出． (2015)
  • Author(s): 倉田 厚、山田正俊、大野慎一郎、橋本浩次、藤田浩司、高梨正勝、黒田雅彦．
  • Organizer: 第104回日本病理学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-04-30 – 2015-05-02
• [Presentation] 肺がん治療を想定した副作用の少ない短鎖型新規核酸医薬の開発. (2015)
  • Author(s): 大野慎一郎、板野華蓮、原田裕一郎、浅田浩太朗、老川桂生、柏迫幹絵、高梨正勝、須藤カツ子、池田徳彦、黒田雅彦.
  • Organizer: 第104回日本病理学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-04-30 – 2015-05-02
• [Presentation] 肺がんにおけるArgonauteファミリーの発現・機能解析． (2015)
  • Author(s): 浅田浩太朗、老川桂生、大野慎一郎、黒田雅彦．
  • Organizer: 第104回日本病理学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-04-30 – 2015-05-02
• [Presentation] microRNA 生成経路を欠損する肺がん細胞株の解析． (2015)
  • Author(s): 老川桂生、浅田浩太朗、大野慎一郎、黒田雅彦．
  • Organizer: 第104回日本病理学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-04-30 – 2015-05-02
• [Presentation] Development of novel form of mimic microRNA for lung cancer therapy. (2015)
  • Author(s): Ohno S, Itano K, Harada Y, Asada K, Oikawa K, Kashiwazako M, Takanashi M, Sudo K, Ikeda N, Kuroda M.
  • Organizer: AACR Annual Meeting 2015
  • Place of Presentation: Philadelphia, Pennsylvania, USA
  • Year and Date: 2015-04-18 – 2015-04-22
• [Presentation] The development of short form of mimic microRNA for lung cancer therapy. (2014)
  • Author(s): Kuroda M
  • Organizer: 26th EORTC-NCI-ACR Symposium
  • Place of Presentation: Barcelona, SPAIN
  • Year and Date: 2014-11-18 – 2014-11-21
• [Presentation] Distinct Phosphorylation Status of TGF-β Recepyor-regulated Smads in NSCLCs with EGFR/Ras Mutations. (2014)
  • Author(s): 牧野洋二郎、尹 晶煥、真村瑞子、黒田雅彦、池田徳彦.
  • Organizer: 第55回日本肺癌学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2014-11-14 – 2014-11-16
• [Presentation] 肺がんを効果的に抑制するMimicmicroRNAの新規形状の探索. (2014)
  • Author(s): 大野慎一郎、板野華蓮、原田裕一郎、高梨正勝、須藤カツ子、池田徳彦、黒田雅彦.
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 核酸医薬品の新展開 Significance and applications of novel class of RNA for cabcer therapy. (2014)
  • Author(s): 黒田雅彦
  • Organizer: 第32回日本ヒト細胞学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2014-08-30 – 2014-08-31
  • Invited
• [Presentation] エクソソームによるドラッグデリバリーシステムの応用 Application of drug delivery systems by exosomes (2014)
  • Author(s): 黒田雅彦、高梨正勝、大野慎一郎.
  • Organizer: 第6回日本RNAi研究会(JARI)、第1回日本細胞外小胞学会(JSEV)
  • Place of Presentation: 広島
  • Year and Date: 2014-08-28 – 2014-08-30
• [Presentation] がんを効果的に抑制する短縮型Mimic microRNA の開発. (2014)
  • Author(s): 大野慎一郎、板野華蓮、原田裕一郎、高梨正勝、須藤カツ子、黒田雅彦
  • Organizer: 第33回分子病理学研究会~宮城蔵王シンポジウム~
  • Place of Presentation: 宮城
  • Year and Date: 2014-07-25 – 2014-07-26
• [Presentation] Colon cancer stem cells and microRNA signature during cokon cancer development. (2014)
  • Author(s): Kuroda M.
  • Organizer: 2nd International Symposium 2014.
  • Place of Presentation: Seoul, KOREA
  • Year and Date: 2014-07-07
  • Invited
• [Presentation] 胃癌細胞株における上皮間葉移行に関与するマイクロRNA200cとその標的の検出 (2014)
  • Author(s): 倉田 厚、山田 正俊、橋本 浩次、藤田 浩司、大野 慎一郎、高梨 正勝、黒田 雅彦.
  • Organizer: 第103回日本病理学会総会
  • Place of Presentation: 広島
  • Year and Date: 2014-04-24 – 2014-04-26
• [Presentation] 自己由細胞由来のエクソソームによるドラッグデリバリーシステムへの応用. (2014)
  • Author(s): 高梨 正勝、上田 しのぶ、須藤 カツ子、石川 章夫、黒田 雅彦.
  • Organizer: 第103回日本病理学会総会
  • Place of Presentation: 広島
  • Year and Date: 2014-04-24 – 2014-04-26
• [Presentation] Novel RNAi agent can control HCV replication. (2014)
  • Author(s): Murakami Y, Ohno S, Kuroda M
  • Organizer: AACR Annual Meeting 2014
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2014-04-05 – 2014-04-09
• [Book] グリオーマ治療のDecision Making 脳神経外科診療プラクティス (2016)
  • Author(s): 上田しのぶ、黒田雅彦
  • Total Pages: 304
  • Publisher: 文光堂
• [Patent(Industrial Property Rights)] プロレニン遺伝子またはプロレニン受容体遺伝子の発現を抑制する一本鎖核酸分子およびその用途 (2016)
  • Inventor(s): 黒田雅彦
  • Industrial Property Rights Holder: 北海道大学、株式会社ボナック、東京医科大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2016-12-29
  • Overseas
• [Patent(Industrial Property Rights)] 遺伝子発現制御のための発現制御核酸分子およびその用途 (2016)
  • Inventor(s): 黒田雅彦、大野慎一郎
  • Industrial Property Rights Holder: 東京医科大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-168499
  • Filing Date: 2016-08-30