Regulation of tissue injury by inflammotion-regulating Macrophages

• Project/Area Number: 26293089
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: Tanaka Masato 東京薬科大学, 生命科学部, 教授 (00294059)
• Co-Investigator(Kenkyū-buntansha): 大村谷 昌樹 熊本大学, 学内共同利用施設等, 准教授 (60398229)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000); Fiscal Year 2016: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
• Keywords: 炎症 / マクロファージ / 虚血再灌流傷害 / 虚血再灌流障害

Outline of Final Research Achievements

In this study, we tried to reveal the mechanisms of immune regulation by CD169-positive macrophages. We analyze the localization and character of these CD169-positive macrophages in various tissues using CD169-Cre-YFP mice. We also examined the pathological roles of these macrophages in ischemic-reperfusion injury of kidney and DSS-induced colitis. In addition, we identified a CD169-positive macrophage-specific transcription factor. We found that this transcription factor not only promotes CCL8 production, but also suppresses gene expression related to anti-inflammation or tissue repair in these macrophages.

Research Products

• [Journal Article] Programmed cell death and the immune system. (2017)
  • Author(s): Nagata S, Tanaka M.
  • Journal Title: Nat Rev Immunol. Volume: 17 Issue: 5 Pages: 333-340
  • DOI: 10.1038/nri.2016.153
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Autophagy suppresses cell migration by degrading GEF-H1, a RhoA GEF (2016)
  • Author(s): T. Yoshida, M. Tsujioka, S. Honda, S. Shimizu
  • Journal Title: Oncotarget Volume: 7 Issue: 23 Pages: 34420-9
  • DOI: 10.18632/oncotarget.8883
  • Peer Reviewed / Open Access
• [Journal Article] Regulation of B cell differentiation by the ubiquitin-binding protein TAX1BP1. (2016)
  • Author(s): Matsushita, N., Suzuki, M., Ikebe, E., Nagashima, S., Inatome, R., Asano, K., Tanaka, M., Matsushita, M., Kondo, E., Iha, H., and Yanagi, S.
  • Journal Title: Sci. Rep Volume: 6 Issue: 1 Pages: 31266-31266
  • DOI: 10.1038/srep31266
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Immune Regulation by Dead Cell Clearance. (2015)
  • Author(s): Tanaka M, Nishitai G.
  • Journal Title: Current Topics in Microbiology and Immunology Volume: ※ Pages: 1-13
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Identification of Pathogenic Cardiac CD11c+ Macrophages in Nod1-Mediated Acute Coronary Arteritis. (2015)
  • Author(s): Motomura Y, et al.
  • Journal Title: Arterioscler Thromb Vasc Biol. Volume: 35 Issue: 6 Pages: 1423-33
  • DOI: 10.1161/atvbaha.114.304846
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Vascular‐resident CD169‐positive Monocytes and Macrophages Control Neutrophil Accumulation in the Kidney with Ischemia‐reperfusion Injury. (2015)
  • Author(s): Karasawa K, Asano K, Moriyama S, Ushiki M, Monya M, Iida M, Kuboki E, Yagita H, Uchida K, Nitta K, Tanaka M.
  • Journal Title: J. Am. Soc. Nephrol. Volume: ２６（４） Issue: 4 Pages: 896-906
  • DOI: 10.1681/asn.2014020195
  • Peer Reviewed / Open Access
• [Journal Article] γ-SNAP stimulates disassembly of endosomal SNARE complexes and regulates endocytic trafficking pathways. (2015)
  • Author(s): Inoue, H., Matsuzaki, Y., Tanaka, A., Hosoi, K., Ichimura, K., Arasaki, K., Wakana, Y., Asano, K., Tanaka, M., Okuzaki, D., Yamamoto, A., Tani, K. and Tagaya, M.
  • Journal Title: J. Cell Sci. Volume: 128 Pages: 2781-2794
  • DOI: 10.1242/jcs.158634
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Intestinal CD169+macrophages initiate mucosal inflammation by secreting CCL8 that recruits inflammatory monocytes (2015)
  • Author(s): Kenichi Asano, Naomichi Takahashi, Mikiko Ushiki, Misa Monya, Fumiaki Aihara, Erika Kuboki,Shigetaka Moriyama,Mayumi Iida,Hiroshi Kitamura, Chun-Hong Qiu,Takashi Watanabe, Masato Tanaka
  • Journal Title: Nat. Commun. Volume: 6 Issue: 1 Pages: 7802-7802
  • DOI: 10.1038/ncomms8802
  • NAID: 120006311913
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Colony-StimulatingFactor-1 Signaling Suppresses Renal Crystal Formation. (2015)
  • Author(s): Taguchi Kazumi, Okada Atsushi, Kitamura Hiroshi, Yasui Takahiro, Naiki Taku, Hamamoto Shuzo, Ando Ryosuke, Mizuno Kentaro, Kawai Noriyasu, Tozawa Keiichi, Asano Kenichi, Tanaka Masato, Miyoshi Ichiro, Kohri Kenjiro
  • Journal Title: Journal of the American Society of Nephrology Volume: 25 Issue: 8 Pages: 1680-1697
  • DOI: 10.1681/asn.2013060675
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Dectin-2 is a direct receptor for mannose-capped lipoarabinomannan of mycobacteria. (2014)
  • Author(s): Yonekawa A, Saijo S, Hoshino Y, Miyake Y, Ishikawa E, Suzukawa M, Inoue H, Tanaka M, Yoneyama M, Oh-Hora M, Akashi K, Yamasaki S.
  • Journal Title: Immunity Volume: 41 Issue: 3 Pages: 402-13
  • DOI: 10.1016/j.immuni.2014.08.005
  • Peer Reviewed / Open Access
• [Journal Article] Marginal zone CD169+ macrophages coordinate apoptotic cell-driven cellular recruitment and tolerance (2014)
  • Author(s): Ravishankar, B., Tanaka, M. (他9名)
  • Journal Title: Proc Natl Acad Sci USA Volume: 111(11) Issue: 11 Pages: 4215-20
  • DOI: 10.1073/pnas.1320924111
  • Peer Reviewed / Open Access
• [Presentation] Immuneregulation by CD169 Macrophages (2016)
  • Author(s): 田中正人
  • Organizer: MMCB2016
  • Place of Presentation: ソラシティ カンファレンスセンター
  • Int'l Joint Research / Invited
• [Presentation] The role of CD169 macrophages in the regulation of inflammation (2016)
  • Author(s): 田中正人
  • Organizer: IMS-JSI国際シンポジウム2016
  • Place of Presentation: パシフィコ横浜
  • Int'l Joint Research / Invited
• [Presentation] 組織傷害におけるCD169マクロファージの役割 (2016)
  • Author(s): 田中正人
  • Organizer: Ｃｅｌｌ Ｄｅａｔｈ 学会
  • Place of Presentation: きゅりあん品川
• [Presentation] CD169陽性マクロファージによる死細胞貪食と炎症制御 (2016)
  • Author(s): 田中正人
  • Organizer: 第89回 生化学会大会
  • Place of Presentation: 仙台国際センター
• [Presentation] 組織マクロファージによる炎症制御機構 (2016)
  • Author(s): 田中正人
  • Organizer: 第31回日本Shock学会総会
  • Place of Presentation: 東京ドームホテル
• [Presentation] CD170陽性マクロファージによる死細胞貪食と免疫制御 (2016)
  • Author(s): 田中正人
  • Organizer: 千里ライフサイエンスセミナーK4
  • Place of Presentation: 千里ライフサイエンスセンタービル
  • Invited
• [Presentation] 細胞死を起点とする生体応答 (2015)
  • Author(s): 田中正人
  • Organizer: 第44回 日本免疫学会
  • Place of Presentation: SORA札幌コンベンションセンター
  • Year and Date: 2015-11-20
• [Presentation] The Role of CD169 Macrophages in immune Regulation (2015)
  • Author(s): 田中正人
  • Organizer: KAI 2015 Fall Conference
  • Place of Presentation: Sejong University Convention Center
  • Year and Date: 2015-11-12
  • Int'l Joint Research / Invited
• [Presentation] 死細胞貪食による免疫制御 (2015)
  • Author(s): 田中正人
  • Organizer: 第31回Wakoワークショップ
  • Place of Presentation: 東京カンファレンスセンター・品川
  • Year and Date: 2015-11-04
  • Invited
• [Presentation] The role of CD169 macrophages in dead cell clearance and inflammatory regulation (2015)
  • Author(s): 田中正人
  • Organizer: Japan Australia Meeting on Cell Death
  • Place of Presentation: Walter Eliza Hall
  • Year and Date: 2015-10-21
  • Int'l Joint Research
• [Presentation] CD169マクロファージによる死細胞貪食と炎症制御 (2014)
  • Author(s): 田中 正人
  • Organizer: 第23回Cell Death学会
  • Place of Presentation: 東京医科歯科大学
  • Year and Date: 2014-07-18 – 2014-07-19