Energy transduction mechanism of the bacterial flagellar protein export apparatus
Project/Area Number |
26293097
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Bacteriology (including mycology)
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Research Institution | Osaka University |
Principal Investigator |
MINAMINO Tohru 大阪大学, 生命機能研究科, 准教授 (20402993)
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Co-Investigator(Renkei-kenkyūsha) |
IMADA Katsumi 大阪大学, 大学院理学研究科, 教授 (40346143)
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Research Collaborator |
KINOSHITA Miki 大阪大学, 大学院生命機能研究科, 特任研究員
INOUE Yumi 大阪大学, 大学院生命機能研究科, 特任技術職員
MORIMOTO Yusuke 大阪大学, 大学院生命機能研究科, 招聘研究員
FUKUMURA Takuma 大阪大学, 大学院生命機能研究科, 大学院生
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
|
Keywords | 電子顕微鏡 / 遺伝学 / 細菌 / 蛋白質 / 一分子計測(SMD) / 感染症 / 一分子計測(SMD) |
Outline of Final Research Achievements |
The bacterial flagellar protein export apparatus, which consists of an export gate complex made of five transmembrane proteins and a cytoplasmic ATPase composed of three cytoplasmic proteins, utilizes ATP and proton motive force across the cytoplasmic membrane as the energy source to drive flagellar protein export. To clarify the energy transduction mechanism of the export apparatus, we have shown that the flagellar export apparatus is a fuel-efficient export engine that can save ATP consumption for the enormous amount of protein export for flagellar assembly and that FlhA acts as an ion channel of the export gate complex to couple the proton flow with protein transport.
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Report
(4 results)
Research Products
(68 results)
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[Journal Article] Bacterial flagella grow through an injection-diffusion mechanism.2017
Author(s)
Renault, T.T., Abraham, A.O., Bergmiller, T., Parados, G., Rainville, S., Charpentier, E., Guet, C.C., Tu, Y., Namba, K., Keener, J.P., Minamino, T., Erhardt, M.
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Journal Title
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Rearrangements of α-helical structures of FlgN chaperone control the binding affinity for its cognate substrates during flagellar type III export.2016
Author(s)
Kinoshita, M., Nakanishi, Y., Furukawa, Y., Namba, K., Imada, K., Minamino, T.
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Journal Title
Mol. Microbiol.
Volume: 101
Issue: 4
Pages: 656-670
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Structural stability of flagellin subunit affects the rate of flagellin export in the absence of FliS chaperone.2016
Author(s)
Furukawa, Y., Inoue, Y., Sakaguchi, A., Mori, Y., Fukumura, T., Miyata, T., Namba, K., Minamino, T.
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Journal Title
Mol. Microbiol.
Volume: 102
Issue: 3
Pages: 405-416
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] FliH and FliI ensure efficient energy coupling of flagellar type III protein export in Salmonella.2016
Author(s)
Minamino T, Kinoshita M, Inoue Y, Morimoto YV, Ihara K, Koya S, Hara N, Nishioka N, Kojima S, Homma M, Namba K
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Journal Title
Microbiologyopen
Volume: -
Issue: 3
Pages: 340-340
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Weak interactions between Salmonella enterica FlhB and other flagellar export apparatus proteins govern type III secretion dynamics2015
Author(s)
McMurry, J.L., Minamino, T., Furukawa, Y., Francis, J.W., Hill, S.A., Helms, K.A., Namba, K.
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Journal Title
PLOS One
Volume: 10
Issue: 8
Pages: e0134884-e0134884
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Conformational rearrangement of FlhA is critical for flagellar type III protein export.2017
Author(s)
Inoue, Y., Kinoshita, M., Kitano, A., Imada, K., Namba, K., Minamino, T.
Organizer
OIST Workshop “Bacterial flagella, injectisomes & type III secretion systems” joint with Annual Flagella Meeting
Place of Presentation
OIST, Kunigami-gun, Okinawa
Year and Date
2017-03-01
Related Report
Int'l Joint Research
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[Presentation] Role of intrinsically disordered region of the C-terminal domain of FliK in substrate specificity switching of the bacterial flagellar type III export apparatus.2017
Author(s)
Kinoshita, M., Aizawa, S., Namba, K., Minamino, T.
Organizer
OIST Workshop “Bacterial flagella, injectisomes & type III secretion systems” joint with Annual Flagella Meeting
Place of Presentation
OIST, Kunigami-gun, Okinawa
Year and Date
2017-03-01
Related Report
Int'l Joint Research
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[Presentation] In Vitro reconstitution of the protein export of the flagellar typeIII secretion system by using the inverted membrane vesicles.2017
Author(s)
Terashima, H., Kawamoto, A., Tatsumi, C., Namba, K., Minamino, T., Imada, K.
Organizer
OIST Workshop “Bacterial flagella, injectisomes & type III secretion systems” joint with Annual Flagella Meeting
Place of Presentation
OIST, Kunigami-gun, Okinawa
Year and Date
2017-03-01
Related Report
Int'l Joint Research
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[Presentation] 蛍光ラベルした細菌べん毛蛋白質のin vitro輸送測定系を用いた検出.2016
Author(s)
戸野侃, 寺島浩行, 巽千夏, 川本晃大, 田端和仁, 上野博史, 松田知己, 永井健治, 野地博行, 難波啓一, 南野徹, 今田勝巳.
Organizer
日本生体エネルギー研究会第42回討論会
Place of Presentation
名古屋工業大学(愛知県名古屋市)
Year and Date
2016-12-20
Related Report
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[Presentation] 蛋白質膜透過を駆動するモータータンパク質のスナップショット. Snapshots of a protein translocation motor.2016
Author(s)
Furukawa, A., Yoshikaie, K., Mori, T., Mori, H., Morimoto, Y., Sugano, Y., Iwaki, S., Minamino, T., Sugita, Y., Tanaka, Y., Tsukazaki, T.
Organizer
第54回日本生物物理学会年会
Place of Presentation
つくば国際会議場(茨城県つくば市)
Year and Date
2016-11-27
Related Report
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[Presentation] FlhAの構造変換がべん毛蛋白質輸送順序の決定に重要である. Conformational rearrangements of FlhA is critical for ordered protein export during flagellar assembly.2016
Author(s)
Inoue, Y., Ogawa, Y., Kinoshita, M., Imada, K., Namba, K., Minamino, T.
Organizer
第54回日本生物物理学会年会
Place of Presentation
つくば国際会議場(茨城県つくば市)
Year and Date
2016-11-27
Related Report
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[Presentation] 細菌べん毛III型タンパク質輸送のin vitro再構築. In vitro reconstitution of the bacterial flagellar type III protein export.2016
Author(s)
Terashima, H., Kawamoto, A., Tatsumi, C., Namba, K., Minamino, T., Imada, K.
Organizer
第54回日本生物物理学会年会
Place of Presentation
つくば国際会議場(茨城県つくば市)
Year and Date
2016-11-27
Related Report
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[Presentation] Direct observation of ring formation of the C-terminal cytoplasmic domain of a flagellar type III export gate protein FlhA by high-speed atomic force microscopy.2016
Author(s)
Minamino, T., Terahara, N., Inoue, Y., Kodera, N., Ando, T., Namba, K.
Organizer
4th Kanazawa Bio-AFM Workshop 2016
Place of Presentation
KKR ホテル金沢(石川県金沢市)
Year and Date
2016-10-05
Related Report
Int'l Joint Research
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[Presentation] Molecular basis for dynamic structural properties of the C ring in the bacterial flagellar motor.2016
Author(s)
Kihoshita, M., Furukawa, Y., Imada, K., Namba, K., Minamino, T.
Organizer
GRC on Sensory Transduction in Microorganisms
Place of Presentation
Ventura Beach Marriott, California, USA
Year and Date
2016-01-17
Related Report
Int'l Joint Research
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[Presentation] バクテリアFlgNシャペロンの機能構造スイッチの分子基盤. Molecular basis for a structural switch of FlgN that regulates its chaperone activity.2015
Author(s)
Kinoshita, M., Nakanishi, Y., Furukawa, Y., Imada, K., Namba, K., Minamino, T.
Organizer
第53回日本生物物理学会年会
Place of Presentation
金沢大学(石川県金沢市)
Year and Date
2015-09-15
Related Report
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[Presentation] Structural characterization of FliP, a component of flagellar type III protein export apparatus.2015
Author(s)
Fukumura, T., Makino, F., Kato, T., Imada, K., Namba, K., Minamino, T.
Organizer
第53回日本生物物理学会年会
Place of Presentation
金沢大学(石川県金沢市)
Year and Date
2015-09-14
Related Report
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[Presentation] 細菌べん毛III型分泌装置のエネルギー変換メカニズム. An energy transduction mechanism of the bacterial flagellar type III secretion apparatus.2015
Author(s)
Terashima, H., Kawamoto, A., Tatsumi, C., Minamino, T., Namba, K., Imada, K.
Organizer
第53回日本生物物理学会年会
Place of Presentation
金沢大学(石川県金沢市)
Year and Date
2015-09-13
Related Report
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