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Molecular mechanism of viral RNP recognition by RIG-I-like receptors

Research Project

Project/Area Number 26293101
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Virology
Research InstitutionChiba University

Principal Investigator

Yoneyama Mitsutoshi  千葉大学, 真菌医学研究センター, 教授 (40260335)

Co-Investigator(Kenkyū-buntansha) 西城 忍  千葉大学, 真菌医学研究センター, 准教授 (60396877)
Co-Investigator(Renkei-kenkyūsha) ONOMOTO Koji  千葉大学, 真菌医学研究センター, 助教 (10612202)
Research Collaborator JOGI Michihiko  
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2016: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Keywordsウイルス / 免疫学 / 核酸 / RNA結合タンパク質 / RNA結合蛋白質 / 自然免疫
Outline of Final Research Achievements

In order to understand how viral RNA sensors, RIG-I-like receptors (RLRs), recognize viral ribonucleoprotein complexes (RNPs) in virus-infected cells, we assessed activation of RIG-I-mediated signaling by viral RNPs using in vitro reconstitution assay system. As a model RNPs, we prepared artificial RNPs of influenza A virus (IAV). In vitro assay indicated that RIG-I-mediated signaling is activated by IAV RNPs, however the data also suggest that host factor(s) is required for full-activation of the signal. The observations would help us to reveal a molecular machinery of activation of anti-viral innate immune responses.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (18 results)

All 2017 2016 2015 2014 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 4 results,  Acknowledgement Compliant: 3 results) Presentation (9 results) (of which Int'l Joint Research: 2 results,  Invited: 7 results) Remarks (3 results)

  • [Journal Article] Regulation of antiviral innate immune signaling by stress-induced RNA granules.2016

    • Author(s)
      Yoneyama M, Jogi M, Onomoto K.
    • Journal Title

      The Journal of Biochemistry

      Volume: 159 Pages: 279-286

    • DOI

      10.1093/jb/mvv122

    • NAID

      40020763323

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Leader-Containing Uncapped Viral Transcript Activates RIG-I in Antiviral Stress Granules.2016

    • Author(s)
      Oh SW, Onomoto K, Wakimoto M, Onoguchi K, Ishidate F, Fujiwara T, Yoneyama M, Kato H, Fujita T.
    • Journal Title

      PLoS Pathogens

      Volume: 12 Issue: 2 Pages: 1-22

    • DOI

      10.1371/journal.ppat.1005444

    • NAID

      120005763384

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Viral RNA detection by RIG-I-like receptors.2015

    • Author(s)
      Yoneyama M, Onomoto K, Jogi M, Akaboshi T, Fujita T.
    • Journal Title

      Curr. Opin. Immunol.

      Volume: 32 Pages: 48-53

    • DOI

      10.1016/j.coi.2014.12.012

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Antiviral innate immunity and stress granule responses.2014

    • Author(s)
      Onomoto K, Yoneyama M, Fung G, Kato H, Fujita T.
    • Journal Title

      Trends Immunol.

      Volume: 35 Issue: 9 Pages: 420-428

    • DOI

      10.1016/j.it.2014.07.006

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel function of human Pumilio proteins in cytoplasmic sensing of viral infection.2014

    • Author(s)
      Narita R, Takahasi K, Murakami E, Hirano E, Yamamoto SP, Yoneyama M, Kato H, Fujita T.
    • Journal Title

      PLoS Pathog.

      Volume: 10 Issue: 10 Pages: 1-11

    • DOI

      10.1371/journal.ppat.1004417

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] DHX36 Enhances RIG-I Signaling by Facilitating PKR-Mediated Antiviral Stress Granule Formation2014

    • Author(s)
      Yoo, J-S., Takahasi, K., Ng, CS., Ouda, R., Onomoto, K., Yoneyama, M., Lai, J-C., Lattmann, S., Nagamine, Y., Matsui, T., Iwabuchi, K., Kato, H. Fujita, T.
    • Journal Title

      PLoS Pathog

      Volume: 10(3) Issue: 3 Pages: 1-18

    • DOI

      10.1371/journal.ppat.1004012

    • NAID

      120005466661

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Viral infection and anti-viral innate immune responses in animal cells2017

    • Author(s)
      Mitsutoshi Yoneyama
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学郡元キャンパス(鹿児島県・鹿児島市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Identification of a novel RNA binding protein that is involved in RLR-meriated signalingovel RNA binding protein that is involved in RLR-meriated signalingo2016

    • Author(s)
      Marie Ban, Koji Onomoto, Mitsutoshi Yoneyama
    • Organizer
      The 15th Awaji International Forum on Infection and Immunity
    • Place of Presentation
      淡路夢舞台国際会議場(兵庫県・淡路市)
    • Year and Date
      2016-09-06
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ウイルス感染に応答したストレス顆粒形成と抗ウイルス生体防御2015

    • Author(s)
      米山光俊
    • Organizer
      BMB2015
    • Place of Presentation
      神戸ポートアイランド(兵庫県・神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] RNAサイレンシングと抗ウイルス応答のクロストーク機構の解析2015

    • Author(s)
      中野悠子、高橋朋子、尾野本浩司、米山光俊、程久美子
    • Organizer
      BMB2015
    • Place of Presentation
      神戸ポートアイランド(兵庫県・神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] Regulation of anti-viral innate immunity by host RNA-binding proteins.2015

    • Author(s)
      Yoneyama, M.
    • Organizer
      第63回日本ウイルス学会学術集会
    • Place of Presentation
      福岡国際会議場(福岡県・福岡市)
    • Year and Date
      2015-11-22
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Molecular machinery of viral RNA recognition by RIG-I-like receptors.2015

    • Author(s)
      Yoneyama, M.
    • Organizer
      13th IGAKUKEN International Symposium on ”Molecular Basis of Viral Disease”
    • Place of Presentation
      東京都医学総合研究所(東京都・世田谷区)
    • Year and Date
      2015-11-20
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Regulation of antiviral innate immunity by the host RNA binding proteins.2014

    • Author(s)
      M. Yoneyama
    • Organizer
      The International Symposium ‘Molecular basis of host cell competency in virus infection’ 2014
    • Place of Presentation
      Pacifico Yokohama(神奈川県・横浜市)
    • Year and Date
      2014-11-08 – 2014-11-09
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 宿主RNA結合タンパク質によるウイルス感染応答制御2014

    • Author(s)
      米山光俊
    • Organizer
      第79回日本インターフェロン・ サイトカイン学会学術集会
    • Place of Presentation
      北海道大学医学部学友会館フラテホール(北海道・札幌市)
    • Year and Date
      2014-06-19 – 2014-06-20
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 細胞内ウイルスRNAセンサーによる感染検知の分子機構2014

    • Author(s)
      米山光俊
    • Organizer
      第15回Pharmaco-Hematologyシンポジウム
    • Place of Presentation
      名古屋市立大学大学院薬学研究科・宮田専治記念ホール(愛知県・名古屋市)
    • Year and Date
      2014-05-23 – 2014-05-24
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Remarks] 千葉大学真菌医学研究センター感染免疫分野ホームページ

    • URL

      http://www.pf.chiba-u.ac.jp/project_immuneresponses/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 千葉大学真菌医学研究センター感染免疫分野ホームページ

    • URL

      http://www.pf.chiba-u.ac.jp/bunya_kansenmeneki/

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
  • [Remarks] 千葉大学真菌医学研究センターホームページ

    • URL

      http://www.pf.chiba-u.ac.jp/index.html

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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