Unifying functional analyses of multiple herpes simplex virus proteins
| Project/Area Number |
26293102
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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| Keywords | ウイルス / HSV / VP26 / カプシド成熟 / 細胞 / 複製 / ICP0 / RanBP10 / ウイルス制御因子 / プロテオーム |
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| Outline of Final Research Achievements |
In this study, we found that (i) an HSV regulatory protein ICP22 interacts with viral nuclear egress complex UL34/UL31 and regulates nuclear export of viral capsids, (ii) an HSV envelope protein gB interacts with a host protein non-muscle-myosin IIB and promotes viral entry, and (iii) an HSV regulatory protein ICP0 interacts with a host transcription factor RanBP10 and regulates viral gene expression at the transcriptional level. We also showed that the functions of an HSV nuclease UL12 is regulated by its phosphorylation at tyrosine 371 and this regulation is critical for viral pathogenicity in vivo. Furthermore, we demonstrated that an HSV protein Us8A plays an important role in viral invasion into the central nervous system from peripheral sites and this Us8A function requires its phosphorylation at serine 61.
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Report
(5 results)
Research Products
(35 results)
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[Journal Article] Herpes Simplex Virus 1 VP22 Inhibits AIM2-Dependent Inflammasome Activation to Enable Efficient Viral Replication.2018
Author(s)
Maruzuru Y, Ichinohe T, Sato R, Miyake K, Okano T, Suzuki T, Koshiba T, Koyanagi N, Tsuda S, Watanabe M, Arii J, Kato A, Kawaguchi Y.
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Journal Title
Cell Host Microbe.
Volume: 23
Issue: 2
Pages: 254-265
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Herpes simplex virus-1 evasion of CD8+ T cell accumulation contributes to viral encephalitis.2017
Author(s)
Koyanagi N, Imai T, Shindo K, Sato A, Fujii W, Ichinohe T, Takemura N, Kakuta S, Uematsu S, Kiyono H, Maruzuru Y, Arii J, Kato A, Kawaguchi Y.
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Journal Title
J Clin Invest.
Volume: 127
Issue: 10
Pages: 3784-3795
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] 2016 International meeting of the Global Virus Network2017
Author(s)
Akkina Ramesh、Ellerbrok Heinz、Hall William、Hasegawa Hideki、Kawaguchi Yasushi、Kleanthous Harold、McSweegan Edward、Mercer Natalia、Romanowski Victor、Sawa Hirofumi、Vahlne Anders
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Journal Title
Antiviral Research
Volume: 142
Pages: 21-29
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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