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Basic study on HIV-1 replication and pathogenicity in host individuals: functional analysis of accessory proteins

Research Project

Project/Area Number 26293104
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Virology
Research InstitutionThe University of Tokushima

Principal Investigator

ADACHI Akio  徳島大学, 大学院医歯薬学研究部, 特命教授 (90127043)

Co-Investigator(Kenkyū-buntansha) 野間口 雅子  徳島大学, 大学院医歯薬学研究部, 准教授 (80452647)
宮崎 恭行  徳島大学, 大学院医歯薬学研究部, 助教 (70607233)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Keywordsウイルス / HIV-1 / HIV-1rmt / アクセサリー蛋白質 / アカゲザル / 抗HIV-1因子 / SIV / HIV-1mt
Outline of Final Research Achievements

It is critically important to establish the rhesus/viral infection system to elucidate HIV-1 replication and pathogenicity in host individuals. We have made every effort to generate CCR5- and rhesus macaque-tropic HIV-1 (HIV-1rmt) clones that grow enough to cause persistent infection and finally induce AIDS. We have successfully obtained molecular HIV-1rmt clones that grow equally or better in rhesus cells relative to the best-growing CXCR4-tropic HIV-1rmt previously described. We thus have advanced to the rhesus-infection experiments to functionally analyze HIV-1 accessory proteins.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (27 results)

All 2016 2015 2014

All Journal Article (11 results) (of which Peer Reviewed: 11 results,  Open Access: 9 results,  Acknowledgement Compliant: 4 results) Presentation (16 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Expression profiles of Vpx/Vpr proteins are co-related with the primate lentiviral lineage.2016

    • Author(s)
      Sakai, Y., Miyake, A., Doi, N., Sasada, H., Miyazaki, Y., Adachi, A, and Nomaguchi, M.
    • Journal Title

      Frontiers in Microbiology

      Volume: 7 Pages: 1211-1211

    • DOI

      10.3389/fmicb.2016.01211

    • NAID

      120006937686

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Phylogenetic insights into the functional relationship between primate lentiviral reverse transcriptase and accessory proteins Vpx/Vpr.2016

    • Author(s)
      Sakai, Y., Doi, N., Miyazaki, Y., Adachi, A, and Nomaguchi, M.
    • Journal Title

      Frontiers in Microbiology

      Volume: 7 Pages: 1655-1655

    • DOI

      10.3389/fmicb.2016.01655

    • NAID

      120006937687

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Natural single-nucleotide variations in the HIV-1 genomic SA1prox region can alter viral replication ability by regulating Vif expression levels.2016

    • Author(s)
      Nomaguchi, M., Doi, N., Sakai, Y., Ode, H., Iwatani, Y., Ueno, T., Matsumoto, Y., Miyazaki, Y., Masuda, T., and Adachi, A.
    • Journal Title

      Journal of Virology

      Volume: 90 Pages: 4563-4578

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Novel mutant human immunodeficiency virus type 1 strains with high degree of resistance to cynomolgus macaque TRIMCyp generated by randam mutagenesis.2016

    • Author(s)
      Sultana, T., Nakayama, E.E., Tobita, S., Yokoyama, M., Seki, Y., Saito, A., Nomaguchi, M., Adachi, A., Akari, H., Sato, H., and Shioda, T.
    • Journal Title

      Journal of General Virology

      Volume: 97 Issue: 4 Pages: 963-976

    • DOI

      10.1099/jgv.0.000408

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Single-amino acid mutation 66SR in Gag-matrix enhances viral single-cycle infectivity of R5-tropic HIV-1rmt.2015

    • Author(s)
      Doi, N., Sakai, Y., Miyazaki, Y., Adachi, A., and Nomaguchi, M.
    • Journal Title

      Journal of Medical Investigation

      Volume: 62 Pages: 228-232

    • NAID

      130005099140

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In silico analysis of HIV-1 Env-gp120 reveals structural bases for viral adaptation in growth-restrictive cells.2015

    • Author(s)
      Yokoyama, M., Nomaguchi, M., Doi, N., Kanda, T., Adachi, A., and Sato, H.
    • Journal Title

      Frontiers in Microbiology

      Volume: 7

    • DOI

      10.3389/fmicb.2016.00110

    • NAID

      120006937684

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Animal model studies on viral infections.2014

    • Author(s)
      Adachi, A., and Miura, T.
    • Journal Title

      Frontiers in Microbiology

      Volume: 5 Pages: 672-672

    • DOI

      10.3389/fmicb.2014.00672

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Distinct combinations of amino acid substitutions in N-terminal domain of Gag-capsid afford HIV-1 resistance to rhesus TRIM5α.2014

    • Author(s)
      Nomaguchi M, Nakayama EE,Yokoyama M, Doi N,Igarashi T, Shioda T, Sato H, Adachi A.
    • Journal Title

      Microbes and Infection

      Volume: 16 Issue: 11 Pages: 936-944

    • DOI

      10.1016/j.micinf.2014.08.017

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Growth properties of macaque-tropic HIV-1 clones carrying <i>vpr/vpx </i>genes derived from simian immunodeficiency viruses in place of their <i>vpr </i>regions2014

    • Author(s)
      Doi, N., Adachi, A., and Nomaguchi, M.
    • Journal Title

      The Journal of Medical Investigation

      Volume: 61 Issue: 3.4 Pages: 374-379

    • DOI

      10.2152/jmi.61.374

    • NAID

      130004822744

    • ISSN
      1343-1420, 1349-6867
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Virological characterization of HIV-2 vpx gene mutants in various cell systems.2014

    • Author(s)
      Nomaguchi, M., Doi, N., and Adachi, A.
    • Journal Title

      Microbes and Infection

      Volume: 16 Issue: 8 Pages: 695-701

    • DOI

      10.1016/j.micinf.2014.06.004

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Natural single-nucleotide polymorphisms in the 3' region of HIV-1 pol gene modulate viral replication ability.2014

    • Author(s)
      Nomaguchi, M. Miyake, A., Doi, N., Fujiwara, S., Miyazaki, Y., Tsunetsugu-Yokota, Y., Yokoyama, M., Sato, H., Masuda, T., and Adachi, A.
    • Journal Title

      Journal of Virology

      Volume: 88 Issue: 8 Pages: 4145-4160

    • DOI

      10.1128/jvi.01859-13

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Effects of mutations HIV-1 Gag-CA helix 7 and linker domain on the virion production.2016

    • Author(s)
      Fujimoto, K., Doi, N., Sakai, Y., Adachi, A., and Nomaguchi, M.
    • Organizer
      第64回日本ウイルス学会学術集会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-10-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] An ultra-low vif type of HIV-1 SA1D2prox variant can adapt and evolve under the high level of APOVEC3G.2016

    • Author(s)
      Adachi, A., Doi, N., Sakai, Y., Fujimoto, K., and Nomaguchi, M.
    • Organizer
      第64回日本ウイルス学会学術集会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-10-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] Identification of cis-elements involved in the HIV-1 vif mRNA production.2016

    • Author(s)
      Nomaguchi, M., Doi, N., Fujimoto, K., Sakai, Y., Nakanishi, S., and Adachi A.
    • Organizer
      第64回日本ウイルス学会学術集会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-10-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] Replication and pathogenicity of HIV-1rmt: towards evaluation of viral growth ability in gut-derived cells.2016

    • Author(s)
      Doi, N., Ishifune, C., Yasutomo, K., Miura, T., Sakai, Y., Fujimoto, K., Harada, S., Yoshimura, K., Nomaguchi, M., and Adachi, A.
    • Organizer
      第64回日本ウイルス学会学術集会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-10-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] Studies on the adaptation process of HIV-1 Env in macaque cells.2016

    • Author(s)
      Sakai, Y., Fujimoto, K., Doi, N., Nomaguchi, M., and Adachi, A.
    • Organizer
      第64回日本ウイルス学会学術集会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-10-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] Novel mutant human immunodeficiency virus type 1 strains with high degree of resistance to cynomolgus macaque TRIMCyp generated by random mutagenesis.2016

    • Author(s)
      Sultana, T., Nakayama, E.E., Tobita, S., Yokoyama, M., Seki, Y., Saito, A., Nomaguchi, M., Adachi, A., Akari, H., Sato, H., Shioda, T.
    • Organizer
      The 23rd Conference on Retroviruses and Opportunistic Infections
    • Place of Presentation
      Boston, USA
    • Year and Date
      2016-02-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] SA1proxの1塩基置換によるHIV-1 Vif発現量変動機構2015

    • Author(s)
      野間口雅子、土肥直哉、吉田知哉、酒井遥介、宮崎恭行、足立昭夫
    • Organizer
      第63回日本ウイルス学会学術集会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      2015-11-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] HIV-1 Vif発現量を増減させるSA1D2prox塩基配列の同定2015

    • Author(s)
      足立昭夫、土肥直哉、酒井遥介、吉田知哉、宮崎恭行、野間口雅子
    • Organizer
      第63回日本ウイルス学会学術集会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      2015-11-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] CCR5-tropic アカゲザル指向性HIV-1の構築とウイルス学的性状解析2015

    • Author(s)
      土肥直哉、野間口雅子、酒井遥介、足立昭夫
    • Organizer
      第63回日本ウイルス学会学術集会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      2015-11-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] HIV-1 Gag-CA I134/I135/S149の1アミノ酸変異はウイルス複製後期過程に影響する2015

    • Author(s)
      酒井遥介、土肥直哉、宮崎恭行、足立昭夫、野間口雅子
    • Organizer
      第63回日本ウイルス学会学術集会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      2015-11-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] Novel mutant HIV-1 strains with high degree of resistance to cynomolgus macaque TRIMCyp generated by random mutagenesis.2015

    • Author(s)
      Sultana, T., Nakayama, E.E., Tobita, S., Saito, A., Nomaguchi, M., Adachi, A., Akari, H., Shioda, T.
    • Organizer
      The 63rd Annual Meeting of The Japanese Society for Virology
    • Place of Presentation
      Fukuoka International Congress Center (Fukuoka, Fukuoka)
    • Year and Date
      2015-11-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] 抗HIV細胞因子と抗HIV戦略.2014

    • Author(s)
      足立昭夫
    • Organizer
      第28回日本エイズ学会学術集会
    • Place of Presentation
      大阪国際会議場(大阪府大阪市)
    • Year and Date
      2014-12-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] In vitro構築系を用いたHIV-1/HIV-2 CA重合能に関する解析.2014

    • Author(s)
      宮崎恭行、泉泰輔、野間口雅子、内山恒夫、足立昭夫
    • Organizer
      第62回日本ウイルス学会学術集会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2014-11-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] HIV-1 Cag-CAへリックス7の変異がウイルス複製後期過程に及ぼす影響の解析.2014

    • Author(s)
      土肥直哉、宮崎恭行、酒井遥介、泉泰輔、内山恒夫、足立昭夫、野間口雅子
    • Organizer
      第62回日本ウイルス学会学術集会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2014-11-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] SA1proxの遺伝子配列はVif/APOBEC3G依存的にウイルス複製能を変動させる.2014

    • Author(s)
      野間口雅子,土肥直哉、酒井遥介、泉泰輔、宮崎恭行、足立昭夫
    • Organizer
      第62回日本ウイルス学会学術集会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2014-11-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] HIV/SIV Vpx蛋白質の発現調節に寄与する領域の解析.2014

    • Author(s)
      酒井遥介、笹田ひかり、土肥直哉、泉泰輔、宮崎恭行、足立昭夫、野間口雅子
    • Organizer
      第62回日本ウイルス学会学術集会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2014-11-10
    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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