Investigation of intractability of pancreatic cancer by using 3D culture of pancreatic stellate cells
| Project/Area Number |
26293119
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied pharmacology
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| Research Institution | Okayama University |
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Principal Investigator |
Mitsunobu Kano 岡山大学, 医歯薬学総合研究科, 教授 (80447383)
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| Co-Investigator(Kenkyū-buntansha) |
正宗 淳 東北大学, 医学系研究科, 准教授 (90312579)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2017: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | 線維化 / 膵臓がん / 三次元培養 / 腫瘍微小環境 / 間質 / 膵星細胞 / 組織・細胞 / 膵がん / 膵癌 / ナノ薬理学 / 腫瘍 / 細胞・組織 / がん / ナノバイオ / 新規モデル |
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| Outline of Final Research Achievements |
The 5-year survival rate of pancreatic cancer is approximately 10% and has not improved for 30 years. Especially in advanced pancreatic cancer, the prognosis is still less than 6 months. The fact suggests that the efficacy of the administered anti-tumor agents are not enough.
In this study, we investigated the role of drug delivery pathway: via 1) tumor blood vessel and 2) tumor fibrous tissue.
For the purpose we constructed a novel three dimensional culture system in this study using cells derived from human pancreatic cancer, especially pancreatic stellate cells (PSC) derived from human patients. We investigated the developed model from the viewpoint of molecular biology and behavior of nanomedicine. As a result, we were able to construct a method of three-dimensional culture / co-culture with reproducibility using PSC, and succeeded in using the model for the analysis of drug delivery.
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Report
(5 results)
Research Products
(26 results)
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[Journal Article] Increased fibrosis and impaired intratumoral accumulation of macromolecules in a murine model of pancreatic cancer co-administered with FGF-2.2016
Author(s)
Sakai, S., Iwata, C., Tanaka, H.Y., Cabral, H., Morishita, Y., Miyazono, K., Kano, M.R.
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Journal Title
J. Control. Release
Volume: 230
Pages: 109-115
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Vascular bursts enhance permeability of tumour blood vessels and improve nanoparticle delivery2016
Author(s)
Yu Matsumoto, Joseph W. Nichols, Kazuko Toh, Takahiro Nomoto, Horacio Cabral, Yutaka Miura, R. James Christie, Naoki Yamada, Tadayoshi Ogura, Mitsunobu R. Kano, Yasuhiro Matsumura, Nobuhiro Nishiyama, Tatsuya Yamasoba, You Han Bae, Kazunori Kataoka
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Journal Title
Nature Nanotechnology
Volume: -
Issue: 6
Pages: 533-538
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Polymeric micelles loaded with platinum anticancer drugs target preangiogenic micrometastatic niches associated with inflammation2014
Author(s)
H. Wu, H. Cabral, K. Toh, P. Mi, Y.-C. Chen, Y. Matsumoto, N. Yamada, X. Liu, H. Kinoh, Y. Miura, M. R. Kano, H. Nishihara, N. Nishiyama, K. Kataoka
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Journal Title
J. Control. Release
Volume: 189
Pages: 1-10
DOI
Related Report
Peer Reviewed
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[Journal Article] Targeted gene delivery by polyplex micelles with crowded PEG palisade and cRGD moiety for systemic treatment of pancreatic tumors2014
Author(s)
Z. Ge, Q. Chen, K. Osada, X. Liu, T. A. Tockary, S. Uchida, A. Dirisala, T. Ishii, T. Nomoto, K. Toh, Y. Matsumoto, M. Oba, M. R. Kano, K. Itaka, K. Kataoka
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Journal Title
Biomaterials
Volume: 35
Issue: 10
Pages: 3416-3426
DOI
Related Report
Peer Reviewed
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[Presentation] Unexpectedness and long-term goals.2015
Author(s)
Kano, MR
Organizer
China-Japan S&T collaboration toward future smart society, China-Japan Interdisciplinary Science and Technology Innovation Salon 2015
Place of Presentation
北京
Year and Date
2015-11-25
Related Report
Int'l Joint Research / Invited
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