A cohort study on the association of physical activity with DNA methylation and inflammatory markers in blood and risk of lifestyle-related diseases

• Project/Area Number: 26293141
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Epidemiology and preventive medicine
• Research Institution: Saga University
• Principal Investigator: Tanaka Keitaro 佐賀大学, 医学部, 教授 (50217022)
• Co-Investigator(Kenkyū-buntansha): 原 めぐみ 佐賀大学, 医学部, 准教授 (90336115) ; 西田 裕一郎 佐賀大学, 医学部, 講師 (50530185) ; 島ノ江 千里 佐賀大学, 医学部, 助教 (10734064) ; 大桃 秀樹 岩手医科大学, その他, 助教 (90453406)
• Co-Investigator(Renkei-kenkyūsha): HIGAKI Yasuki 福岡大学, スポーツ科学部, 教授 (10228702)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2014: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
• Keywords: 身体活動 / 末梢血DNAメチル化 / 炎症マーカー / 生活習慣病 / 脳梗塞 / ASC遺伝子 / コホート研究 / 分子遺伝疫学 / DNAメチル化解析 / 全身性炎症

Outline of Final Research Achievements

We examined the association of accelerometer-determined physical activity (PA) with ASC gene methylation in white blood cells, based on a cohort study of about 12 thousand residents in Saga city. We also evaluated the association between PA and ischemic stroke and if it is mediated by ASC methylation. In cross-sectional and longitudinal studies, PA (particularly with low intensity) was positively associated with ASC methylation while ASC methylation was inversely associated with blood inflammatory markers. In a case-cohort study, neither an association between PA and ischemic stroke nor a mediating role of ASC methylation was evident. PA may cause higher ASC methylation, thereby leading to lower systemic inflammation.

Research Products

• [Journal Article] Perceived stress, depressive symptoms, and oxidative DNA damage (2018)
  • Author(s): Shimanoe C, Hara M, Nishida Y, Nanri, H, Horita M, Yamada Y, Li YS, Kasai H, Kawai K, Higaki Y, Tanaka K
  • Journal Title: Psychosomatic Medicine Volume: 80 Issue: 1 Pages: 28-33
  • DOI: 10.1097/psy.0000000000000513
  • Peer Reviewed / Open Access
• [Journal Article] Coping strategy and social support modify the association between perceived stress and C-reactive protein: a longitudinal study of healthy men and women (2018)
  • Author(s): Shimanoe C, Hara M, Nishida Y, Nanri H, Otsuka Y, Horita M, Yasukata J, Miyoshi N, Yamada Y, Higaki Y, Tanaka K
  • Journal Title: Stress Volume: 21 Issue: 3 Pages: 237-246
  • DOI: 10.1080/10253890.2018.1435638
  • Peer Reviewed
• [Journal Article] A genome-wide association study of coping behaviors suggests FBXO45 is associated with emotional expression (2018)
  • Author(s): Shimanoe C, Hachiya T, Hara M, Nishida Y, Tanaka K, Sutoh Y, Shimizu A, et al
  • Journal Title: Genes, Brain and Behavior Volume: 印刷中 Issue: 2
  • DOI: 10.1111/gbb.12481
  • Peer Reviewed
• [Journal Article] Development of suitable method for large-scale urinary glucocorticoid analysis by liquid chromatography-mass spectrometry (2017)
  • Author(s): Matsumoto A, Shimanoe C, Tanaka K, Ichiba M, Hara M
  • Journal Title: J. Chromatography B Volume: 1057 Pages: 62-69
  • DOI: 10.1016/j.jchromb.2017.04.031
  • Peer Reviewed / Open Access
• [Journal Article] "Influence of cigarette smoking and inflammatory gene polymorphisms on glycated hemoglobin in the Japanese general population" (2016)
  • Author(s): Nishida Y, Hara M, Sakamoto T, Shinchi K, Kawai S, Naito M, Hamajima N, Kadota A, Suzuki S, Ibusuki R, Hirata A, Yamaguchi M, Kuriyama N, Oze I, Mikami H, Kubo M, Tanaka H.
  • Journal Title: Prev. Med. Rep. Volume: 3 Pages: 288-295
  • DOI: 10.1016/j.pmedr.2016.03.010
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Factors Associated With Non-participation in a Face-to-Face Second Survey Conducted 5 Years After the Baseline Survey (2015)
  • Author(s): Hara M, Shimanoe C, Otsuka Y, Nishida Y, Nanri H, Horita M, Yasukata J, Miyoshi N, Yamada Y, Higaki Y, Tanaka K.
  • Journal Title: Journal of Epidemiology Volume: 25 Issue: 2 Pages: 117-125
  • DOI: 10.2188/jea.JE20140116
  • NAID: 130004704585
  • ISSN: 0917-5040, 1349-9092
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Genetic variants of SLC17A1 are associated with cholesterol homeostasis and hyperhomocysteinaemia in Japanese men (2015)
  • Author(s): Koyama, T. Matsui, D. Kuriyama, N. Ozaki, E. Tanaka, K. Oze, I. Hamajima, N. Wakai, K. Okada, R. Arisawa, K. Mikami, H. Shimatani, K. Hirata, A. Takashima, N. Suzuki, S. Nagata, C. Kubo, M. Tanaka, H.
  • Journal Title: Sci Rep Volume: 5 Issue: 1 Pages: 15888-15888
  • DOI: 10.1038/srep15888
  • NAID: 120006875676
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Dietary Patterns and Serum Gamma-Glutamyl Transferase in Japanese Men and Women (2015)
  • Author(s): Nanri H, Hara M, Nishida Y, Shimanoe C, Nakamura K, Higaki Y, Imaizumi T, Taguchi N, Sakamoto T, Horita M, Shinchi K, Kokaze A, Tanaka K.
  • Journal Title: Journal of Epidemiology Volume: 25 Issue: 5 Pages: 378-386
  • DOI: 10.2188/jea.JE20140158
  • NAID: 130005067867
  • ISSN: 0917-5040, 1349-9092
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Perceived stress and coping strategies in relation to body mass index: cross-sectional study of 12,045 Japanese men and women. (2015)
  • Author(s): Shimanoe C, Hara M, Nishida Y, Nanri H, Otsuka Y, Nakamura K, Higaki Y, Imaizumi T, Taguchi N, Sakamoto T, Horita M, Shinchi K, Tanaka K.
  • Journal Title: PLoS One Volume: 12 Issue: 2 Pages: e0118105-e0118105
  • DOI: 10.1371/journal.pone.0118105
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Objectively measured physical activity and inflammatory cytokine levels in middle-aged Japanese people. (2014)
  • Author(s): Nishida Y, Higaki Y, Taguchi N, Hara M, Nakamura K, Nanri H, Imaizumi T, Sakamoto T, Horita M, Shinchi K, Tanaka K.
  • Journal Title: Preventive Medicine Volume: 64 Pages: 81-87
  • DOI: 10.1016/j.ypmed.2014.04.004
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 日本人成人の余暇時間の運動に関するGWAS研究：J-MICC Study (2018)
  • Author(s): 原めぐみ、八谷剛史、西田裕一郎、島ノ江千里、清水厚志、田中恵太郎
  • Organizer: 第28回日本疫学会学術総会
• [Presentation] 日常身体活動とPYCARD遺伝子メチル化の縦断的関連 (2018)
  • Author(s): 西田裕一郎、原めぐみ、桧垣靖樹、田口尚人、島ノ江千里、南里妃名子、中村和代、今泉猛、坂本龍彦、安方惇、三好伸幸、山田陽介、堀田美加子、東元健、副島英伸、新地浩一、田中恵太郎
  • Organizer: 第28回日本疫学会学術総会
• [Presentation] 教育歴と精神ストレスおよび抑うつとの関連について：J-MICC study SAGA (2018)
  • Author(s): 島ノ江 千里、原 めぐみ、西田 裕一郎、南里 妃名子、安方 惇、山田陽介、桧垣靖樹、田中 恵太郎
  • Organizer: 第28回日本疫学会学術総会
• [Presentation] 5年間の精神ストレスとCRPの関連は、対処行動や社会的支援の変化により異なる (2018)
  • Author(s): 島ノ江 千里、原 めぐみ、西田 裕一郎、南里 妃名子、安方 惇、三好信行、山田陽介、桧垣靖樹、田中 恵太郎
  • Organizer: 第88回日本衛生会学術総会
• [Presentation] Mitochondrial haplogroups modify the effect of physical activity on urinary levels of 8-hydroxydeoxyguanosine in middle-aged Japanese subjects (2017)
  • Author(s): Hara M, Nishida Y, Fuku N, Shimanoe C, Nanri H, Taguchi N, Kawai K, Li YS, Kasai H, Higaki Y, Tanaka K
  • Organizer: The 21st International Epidemiology Association (IEA) ,World Congress of Epidemiology (WCE2017).
  • Int'l Joint Research
• [Presentation] Association between objectively measured physical activity and methylation of PYCARD gene in the Japanese general population (2017)
  • Author(s): Yuichiro Nishida, Yasuki Higaki, Naoto Taguchi, Megumi Hara, Kazuyo Nakamura, Hinako Nanri, Takeshi Imaizumi, Tatsuhiko Sakamoto, Mikako Horita, Koichi Shinchi, Chisato Shimanoe, Ken Higashimoto, Hidenobu Soejima, Keitaro Tanaka
  • Organizer: The 21st International Epidemiological Association (IEA) World Congress of Epidemiology
  • Int'l Joint Research
• [Presentation] 精神ストレスと睡眠の質の関連における情緒的支援の影響は、就業状況によって異なる (2017)
  • Author(s): 島ノ江 千里、原 めぐみ、西田 裕一郎、南里 妃名子、安方 惇、山田陽介、桧垣靖樹、田中 恵太郎
  • Organizer: 第23回日本行動医学会学術総会
• [Presentation] Perceived stress, depressive state, and oxidative DNA damage in a general Japanese population. (2017)
  • Author(s): Shimanoe C, Hara M, Nishida Y, Nanri H, Horita M, Yamada Y, Li Y S, Kasai H, Kawai K, Higaki Y, Tanaka K
  • Organizer: The 21st International Epidemiology Association (IEA) ,World Congress of Epidemiology (WCE2017).
  • Int'l Joint Research
• [Presentation] 心理社会的因子と生活習慣病に介在するバイオマーカーの疫学的検討 ―日本多施設共同コホート研究（J-MICC-study）SAGA－ (2017)
  • Author(s): 島ノ江 千里
  • Organizer: 日本心理学会第81回大会
• [Presentation] Amino acid replacement (K14Q) of mitochondria-derived MOTS-c affects type 2 diabetes in men with lower physical activity (2017)
  • Author(s): Zempo H, Fuku N, Kumagai H, Nishida Y, Higaki Y, Naito H, Hara M, Tanaka K
  • Organizer: The 3rd Congress, International Academy of Sportology
  • Int'l Joint Research
• [Presentation] ミトコンドリアDNA由来ペプチドMOTS-cのアミノ酸置換(K14Q)は身体活動量の低い男性における2型糖尿病に関連する (2017)
  • Author(s): 膳法浩史、福典之、熊谷仁、西田裕一郎、桧垣靖樹、内藤久士、原めぐみ、田中恵太郎
  • Organizer: 第72回日本体力医学会大会
• [Presentation] 加齢とhOGG1遺伝子プロモーター領域のメチル化の関連. (2016)
  • Author(s): 原めぐみ、西田裕一郎、島ノ江千里、堀田美加子、田中恵太郎.
  • Organizer: 第26回日本疫学会学術総会
  • Place of Presentation: 鳥取県米子市
  • Year and Date: 2016-01-21
• [Presentation] 長期的な自覚ストレスとDNA酸化損傷（尿中8OHdG）との関連は抑うつの高さによって異なる：J-MICC study SAGA. (2016)
  • Author(s): 島ノ江千里、原めぐみ、西田裕一郎、大塚康子、南里妃名子、堀田美加子、安方淳、三好信行、山田陽介、Li Yun-Shan、河井一明、葛西宏、桧垣靖樹、田中恵太郎.
  • Organizer: 第26回日本疫学会学術総会
  • Place of Presentation: 鳥取県米子市
  • Year and Date: 2016-01-21
• [Presentation] 5年間の自覚ストレスとDNA損傷マーカー（8OHdG）との関連―JMICC study SAGA. (2015)
  • Author(s): 島ノ江 千里、原 めぐみ、西田 裕一郎、大塚康子、南里 妃名子、堀田 美加子、安方 淳、三好信行、山田陽介、Yun-Shan Li、河井一明、葛西 宏、桧垣靖樹、田中 恵太郎.
  • Organizer: 第22回日本行動医学会学術総会
  • Place of Presentation: 宮城県仙台市
  • Year and Date: 2015-10-15
• [Presentation] Association between moderate/vigorous intensity of physical activity and urinary levels of 8-hydroxydeoxyguanosine. (2015)
  • Author(s): Hara M, Nishida Y, Shimanoe C, Otsuka Y, Nanri H, Higaki Y, Horita M, Kawai K, Li YS, Kasai J, Kasai H, Tanaka K.
  • Organizer: 第25回日本疫学会学術総会
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2015-01-21 – 2015-01-23
• [Presentation] Association between objectively measured physical activity and serum adiponectin in the middle-aged population. (2015)
  • Author(s): Nishida Y, Higaki Y, Taguchi N, Hara M, Nakamura K, Nanri H, Imaizumi T, Sakamoto T, Shimanoe C, Horita M, Shinchi K, Tanaka K.
  • Organizer: 第25回日本疫学会学術総会
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2015-01-21 – 2015-01-23
• [Presentation] 生活習慣病の遺伝子環境交互作用の解明を目指して：日本多施設共同コーホート研究－佐賀地区－(教育講演）. (2014)
  • Author(s): 田中恵太郎.
  • Organizer: 第17回日本運動疫学会学術総会
  • Place of Presentation: 長崎県長崎市
  • Year and Date: 2014-09-18
  • Invited
• [Remarks] 佐賀大学医学部社会医学講座予防医学分野ホームページ
  • URL: http://www.prevent.med.saga-u.ac.jp/index.html
• [Remarks] J-MICC Study（ジェイミック・スタディ）佐賀フィールドとは
  • URL: http://www.prevent.med.saga-u.ac.jp/jmiccsaga.html