Analysis of the methylation status of leukocytes DNA stored in a longitudial cohort study

• Project/Area Number: 26293144
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Epidemiology and preventive medicine
• Research Institution: Fujita Health University
• Principal Investigator: Suzuki Koji 藤田保健衛生大学, 保健学研究科, 教授 (60288470)
• Co-Investigator(Kenkyū-buntansha): 山田 宏哉 藤田保健衛生大学, 医学部, 助教 (80610352) ; 橋本 修二 藤田保健衛生大学, 医学部, 教授 (50148334)
• Co-Investigator(Renkei-kenkyūsha): HAMAJIMA Nobuyuki 名古屋大学, 医学系研究科, 教授 (30172969)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2014: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
• Keywords: エピジェネティクス / DNAメチル化 / コホート / 生活習慣病 / バイオマーカー / 社会医学 / メチル化 / 遺伝子 / コホート研究 / 一般住民 / ＤＮＡメチル化

Outline of Final Research Achievements

DNA methylation is one of epigenetic mechanism that regulate gene expression without alternations of DNA sequences. Alteration of DNA methylation is implicated to the regulation of genetic expression and several diseases. Methylation of long interspersed nuclear element-1 (LINE-1) LINE-1 has been associated with genome instability, and is a surrogate maker of global DNA methylation. We established a method for measuring leukocyte LINE-1 DNA methylation level and examined whether LINE-1 methylation is associated with metabolic syndrome in cross-sectional study and whether LINE-1 methylation related to mortality rate. We found that low LINE-1 DNA methylation in leukocyte was significantly associated with metabolic syndrome and dyslipidemia. In longitudinal study, high methylation status of LINE-1 in leukocyte is significantly associated with high cancer mortality rate. Alteration of DNA methylation may affect subsequent cancer deaths.

Research Products

• [Presentation] 住民健診受診者における白血球DNAメチル化レベルと糖代謝指標値との関連 (2017)
  • Author(s): 鈴木康司, 山田宏哉, 宗綱栄二, 山崎未来, 坪井良樹, 近藤真里, 水野元貴, 村瀬悠里, 近藤奏子, 太田駿瑠, 大橋鉱二, 石川浩章, 井上 孝, 橋本修二, 浜島信之
  • Organizer: 第27日本疫学会学術総会
  • Place of Presentation: ベルクラシック甲府（山梨県甲府市）
  • Year and Date: 2017-01-25
• [Presentation] 住民健診受診者における白血球DNAメチル化率と 脂質異常症との関連 (2017)
  • Author(s): 坪井良樹, 山田宏哉, 宗綱栄二, 山崎未来, 近藤真里, 水野元貴, 村瀬悠里, 近藤奏子, 太田駿瑠, 大橋鉱二, 石川浩章, 井上 孝, 橋 修二, 浜島信之, 鈴木康司
  • Organizer: 第27日本疫学会学術総会
  • Place of Presentation: ベルクラシック甲府（山梨県甲府市）
  • Year and Date: 2017-01-25
• [Presentation] LINE-1 DNA methylation levels in leukocyte and hyperglycemia in a Japanese population. (2016)
  • Author(s): Suzuki K., Yamada H., Munetsuna E., Yamazaki M., Tsuboi Y., Kondo K., Ohashi K., Ishikawa H., Mizuno G., Murase Y., Kondo K., Ichino N., Sugimoto K., Osakabe K., Inoue T., Hashimoto S., Hamajima N.
  • Organizer: 14th Asia-Pacific Federation for Clinical Biochemistry and Laboratory Medicine Congress
  • Place of Presentation: Taipei (China)
  • Year and Date: 2016-11-26
  • Int'l Joint Research
• [Presentation] Association between LINE-1 DNA methylation levels in leukocyte and metabolic syndrome in a Japanese population. (2016)
  • Author(s): Tsuboi Y., Yamada H., Munetsuna E., Yamazaki M., Mizuno G., Murase Y., Kondo K., Ota T., Ohashi K., Ishikawa H., Ichino N., Sugimoto K., Osakabe K., Kondo M., Inoue T., Suzuki K.
  • Organizer: 14th Asia-Pacific Federation for Clinical Biochemistry and Laboratory Medicine Congress
  • Place of Presentation: Taipei (China)
  • Year and Date: 2016-11-26
  • Int'l Joint Research