| Project/Area Number |
26293170
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General internal medicine(including psychosomatic medicine)
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
副島 一孝 日本大学, 医学部, 准教授 (00246589)
加野 浩一郎 日本大学, 生物資源科学部, 教授 (80271039)
風間 智彦 日本大学, 医学部, 助手 (80525668)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Keywords | 脱分化脂肪細胞 / 細胞治療 / 間葉系幹細胞 / 再生医療 / エクソソーム / 再生医学 / 脂肪細胞 |
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| Outline of Final Research Achievements |
In the present study, we examined the characteristics and therapeutic potential of dedifferentiated fat (DFAT) cell-derived exosomes. As results, we found that DFAT cell-derived exosomes contained a variety of microRNAs that are thought to play important roles in intercellular communications. The expression profile of microRNAs in DFAT cell-derived exosomes was very similar to that in adipose-derived stem cell (ASC)-derived exosomes. DFAT cell-derived exosomes inhibited proliferation of T lymphocytes and promoted differentiation of naive T cells into regulatory T cells. In addition, DFAT cell-derived exosomes stimulated proliferation of nucleus pulposus cells of intervertebral disc and increased expression of SOX9, a critical transcription factor for chondrogenesis. These results suggest that DFAT cell-derived exosomes have therapeutic potential for T cell-mediated autoimmune disorders and intervertebral disc degeneration.
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