| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
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| Outline of Final Research Achievements |
Chronic infection of hepatitis B virus (HBV, HCV) is a major cause of cirrhosis, liver cancer etc in Japan. However, it was difficult to analyze because of the species specificity of its infectivity. In this study, for the purpose of deriving hepatocytes from human iPS cells and constructing an HBV infection analysis system, 1) preparation of highly expressed human hepatocytes of HBV receptors, 2) genes responsible for high function of hepatocytes Screening, etc. were conducted. As a result, it has been found that HBV is efficiently infected / amplified in human iPS cell-derived hepatocytes overexpressing Ntcp, and that KLF 15 is important for enhancing hepatocyte function.
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