| Project/Area Number |
26293188
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cardiovascular medicine
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| Research Institution | Osaka University |
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Principal Investigator |
LEE JONG-KOOK 大阪大学, 医学(系)研究科(研究院), 特任准教授(常勤) (60303608)
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| Co-Investigator(Kenkyū-buntansha) |
宮川 繁 大阪大学, 医学(系)研究科(研究院), 特任准教授(常勤) (70544237)
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| Research Collaborator |
MIWA Keiko 大阪大学, 医学系研究科, 特任助教(常勤)
YOKOYAMA Teruki 大阪大学, 医学系研究科, 大学院生
TOMOYAMA Satoki 大阪大学, 医学系研究科, 大学院生
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
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| Keywords | ３次元組織構築 / 脱細胞化 / 細胞外マトリックス / 心筋細胞 / iPS細胞 / 光学マッピング / 興奮伝播 / 組織透明化 / 脱細胞化心臓マトリックス / 細胞接着因子 / 組織透明化技術 / 再生医学 / 組織工学 / 脱細胞化マトリックス / 臓器構築 / 3次元心筋組織 / 移植 |
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| Outline of Final Research Achievements |
Adult rat hearts were decellularized with the perfusion of detergents, and then extracellular matrices were recellularized with cardiac cells from neonatal rats. We evaluated functional and morphological properties of organ-like three dimensional heart tissues using optical mapping and other methods. The results were published in an international journal of biomaterial sciences（Yasui et al. Biomaterials. 2014; IF 8.387）. Furthermore, we succeeded in observing coronary vessels and sympathetic nerves of entire murine hearts using tissue-clearing techniques. The technique can be utilized to evaluate detailed morphological properties of organ-like three-dimensional heart tissues. (Yokoyama et al. Presented in AHA2016 etc. The manuscript is in review.)
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