Development of an innovative lymphocyte chip to establish personalized immuno-therapy for infectious diseases

• Project/Area Number: 26293237
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Infectious disease medicine
• Research Institution: University of Toyama
• Principal Investigator: MURAGUCHI Atsushi 富山大学, 大学院医学薬学研究部(医学), 教授 (20174287)
• Co-Investigator(Kenkyū-buntansha): 岸 裕幸 富山大学, 大学院医学薬学研究部(医学), 准教授 (60186210) ; 小澤 龍彦 富山大学, 大学院医学薬学研究部(医学), 助教 (10432105) ; 浜名 洋 富山大学, 大学院医学薬学研究部(医学), 助教 (90551549) ; 小林 栄治 富山大学, 大学院医学薬学研究部(医学), 助教 (70459733)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000); Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2015: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2014: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
• Keywords: リンパ球チップ / Tリンパ球 / T細胞受容体 / 免疫治療 / 個の免疫 / Ｔリンパ球 / Ｔ細胞受容体

Outline of Final Research Achievements

To develop an innovative lymphocyte chip to establish personalized immuno-therapy for infectious diseases, 1) We developed a new lymphocyte chip using microwave-coated chip that were able to efficiency trap single microwave-beads coated lymphocytes. 2) We succeeded in retrieving a single antigen-specific T lymphocytes from lymphocyte chip and efficiently analyzing TCR gene sequences of alpha- and beta-TCR gene. 3) We succeeded in detection of antigen-specific CD4-positive T lymphocytes using lymphocyte chip. 4) We improved a method for verifying TCR function using various target cell lines. 5) We successfully constructed sTCR ( a dimer containing TCR V-region and TCR C-region) and scFv-TCR ( a monomer containing V-alpha gene and V-beta gene in a single polypeptide). 6) We established 4 kinds of human anti-West Nile virus monoclonal antibodies with neutralizing activities.

Research Products

• [Journal Article] Association Between High-Avidity T-Cell Receptors, Induced by α-Fetoprotein-Derived Peptides, and Anti-Tumor Effects in Patients With Hepatocellular Carcinoma (2017)
  • Author(s): Nakagawa H, Mizukoshi E, Kobayashi E, Tamai T, Hamana H, Ozawa T, Kishi H, Kitahara M, Yamashita T, Arai K, Terashima T, Iida N, Fushimi K, Muraguchi A, Kaneko S
  • Journal Title: Gastroenterology Volume: 152 Issue: 6 Pages: 1395-1406
  • DOI: 10.1053/j.gastro.2017.02.001
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification of an HLA class I allele closely involved in the auto-antigen presentation in acquired aplastic anemia (2017)
  • Author(s): Zaimoku Y, Takamatsu H, Hosomichi K, Ozawa T, Nakagawa N, Imi T, Maruyama H, Katagiri T, Kishi H, Tajima A, Muraguchi A, Kashiwase K, Nakao S
  • Journal Title: Blood Volume: 印刷中 Issue: 21 Pages: 2908-2916
  • DOI: 10.1182/blood-2016-11-752378
  • Peer Reviewed / Open Access
• [Journal Article] A novel, rapid and efficient method of cloning functional antigen-specific T-cell receptors from single human and mouse T-cells (2016)
  • Author(s): Hamana H, Shitaoka K, Kishi H, Ozawa T, Muraguchi A
  • Journal Title: Biochem Biophys Res Commun Volume: 474 Issue: 4 Pages: 709-714
  • DOI: 10.1016/j.bbrc.2016.05.015
  • Peer Reviewed
• [Journal Article] TRAIL-receptor 1 IgM antibodies strongly induce apoptosis in human cancer cells in vitro and in vivo. (2016)
  • Author(s): Piao X, Ozawa T, Hamana H, Shitaoka K, Jin A, Kishi H, Muraguchi A.
  • Journal Title: Oncoimmunology Volume: 5 Issue: 5 Pages: e1131380-e1131380
  • DOI: 10.1080/2162402x.2015.1131380
  • Peer Reviewed
• [Journal Article] Fully human monoclonal antibodies to TRAIL-R1 enhance TRAIL-induced apoptosis via activation of caspase-8 pathway. (2016)
  • Author(s): Hao Z, Han X, Sun X, Shen M, Huang J, Li Y, Ozawa T, Pang D, Jin S, Kishi H, Muraguchi A, Jin A.
  • Journal Title: Biochem Biophys Res Commun. Volume: 475 Issue: 2 Pages: 238-44
  • DOI: 10.1016/j.bbrc.2016.05.089
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Capture of esophageal and breast cancer cells with polymeric microfluidic devices for CTC isolation. (2016)
  • Author(s): Ohnaga T, Shimada Y, Takata K, Obata T, Okumura T, Nagata T, Kishi H, Muraguchi A, Tsukada K.
  • Journal Title: Mol Clin Oncol. Volume: 4 Pages: 599-602
  • Peer Reviewed / Open Access
• [Journal Article] Identification of broadly conserved cross-species protective Leishmania antigen and its responding CD4 + T cells (2015)
  • Author(s): Z. Mou, J. Li, T. Boussoffara, H. Kishi, H. Hamana, P. Ezzati, C. Hu, W. Yi, D. Liu, F. Khadem, I. Okwor, P. Jia, K. Shitaoka, S. Wang, M. Ndao, C. Petersen, J. Chen, S. Rafati, H. Louzir, A. Muraguchi, J. a Wilkins, and J. E. Uzonna,
  • Journal Title: Science Translational Medicine Volume: 7 Issue: 310 Pages: 1-13
  • DOI: 10.1126/scitranslmed.aac5477
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Immunological features of T cells induced by human telomerase reverse transcriptase-derived peptides in patients with hepatocellular carcinoma. (2015)
  • Author(s): E Mizukoshi, H Nakagawa, M Kitahara, T Yamashita, K Arai, H Sunagozaka, K Fushimi, E Kobayashi, H Kishi, A Muraguchi, S Kaneko.
  • Journal Title: Cancer Lett Volume: 364 Issue: 2 Pages: 98-105
  • DOI: 10.1016/j.canlet.2015.04.031
  • Peer Reviewed / Open Access
• [Journal Article] Production of monoclonal antibodies against GPCR using cell-free synthesized GPCR antigen and biotinylated liposome-based interaction assay (2015)
  • Author(s): Takeda H, Ogasawara T, Ozawa T, Muraguchi A, Jih PJ, Morishita R, Uchigashima M, Watanabe M, Fujimoto T, Iwasaki T, Endo Y, Sawasaki T
  • Journal Title: Sci Rep Volume: 5 Issue: 1 Pages: 11333-11333
  • DOI: 10.1038/srep11333
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A Human Anti-M2 Antibody Mediates Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) and Cytokine Secretion by Resting and Cytokine-Preactivated Natural Killer (NK) Cells (2015)
  • Author(s): Venkateswara R. Simhadri, Milena Dimitrova, John L. Mariano, Olatz Zenarruzabeitia, Weimin Zhong, Tatsuhiko Ozawa, Atsushi Muraguchi, Hiroyuki Kishi, Maryna C. Eichelberger, Francisco Borrego
  • Journal Title: PLoS One Volume: 10 Issue: 4 Pages: e0124677-e0124677
  • DOI: 10.1371/journal.pone.0124677
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Monoclonal Antibody Against Citrullinated Peptides Obtained From Rheumatoid Arthritis Patients Reacts With Numerous Citrullinated Microbial and Food Proteins (2015)
  • Author(s): Reina Tsuda, Tatsuhiko Ozawa, Eiji Kobayashi, Hiroshi Hamana, Hirofumi Taki, Kazuyuki Tobe, Eiji Sugiyama, Masahiro Iwamoto, Johji Imura, Hiroyuki Kishi, Atsushi Muraguchi
  • Journal Title: Arthritis & Rheumatology Volume: 67 Issue: 8 Pages: 2020-2031
  • DOI: 10.1002/art.39161
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A chimeric antigen receptor for TRAIL-receptor 1 induces apoptosis in various types of tumor cells. (2014)
  • Author(s): Kobayashi E, Kishi H, Ozawa T, Hamana H, Nakagawa H, Jin A, Lin Z, Muraguchi A.
  • Journal Title: Biochem Biophys Res Commun. Volume: 453 Issue: 4 Pages: 798-803
  • DOI: 10.1016/j.bbrc.2014.10.024
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] TCR repertoire of CD4+CD25+CD137+Foxp3+ tumor infiltrating lymphocytes in mice. (2016)
  • Author(s): Kishi H, Hamana H, Shitaoka K, Lyu F, Ozawa T, Muraguchi A.
  • Organizer: 第45回日本免疫学会学術集会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県宜野湾市）
  • Year and Date: 2016-12-05
• [Presentation] Single-T-cell manipulation method using microwell array chip (T-ISAAC) allows rapid and efficient cloning of antigen-specific TCRs. (2016)
  • Author(s): Ozawa T, Kishi H, Hamana H, Tajiri K, Muraguchi A.
  • Organizer: 第45回日本免疫学会学術集会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県宜野湾市）
  • Year and Date: 2016-12-05
• [Presentation] A rapid and easy system for cDNAs cloning of antigen specific TCRs from single human and mouse T-cells within 4 days. (2016)
  • Author(s): Hamana H, Kishi H, Shitaoka K, Ozawa T, Muraguchi A.
  • Organizer: 第45回日本免疫学会学術集会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県宜野湾市）
  • Year and Date: 2016-12-05
• [Presentation] T cell receptor obtained from tumor-infiltrating lymphocytes without using tumor-specific antigens exhibits cytotoxicity to tumors in vitro and in vivo. (2016)
  • Author(s): Shitaoka K, Kishi H, Hamana H, Hayakawa Y, Ozawa T, Muraguchi A.
  • Organizer: 第45回日本免疫学会学術集会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県宜野湾市）
  • Year and Date: 2016-12-05
• [Presentation] TCR repertoire analysis of OVA-specific T-cells infiltrating into OVA-expressing-melanoma during tumor progression. (2016)
  • Author(s): Lyu F, Shitaoka K, Hamana H, Hayakawa Y, Kishi H, Muraguchi A.
  • Organizer: 第45回日本免疫学会学術集会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県宜野湾市）
  • Year and Date: 2016-12-05
• [Presentation] Epitopes and tissue reactivity of monoclonal anti-TRIM21/Ro52 autoantibodies isolated from collagen disease-patients with interstitial lung disease. (2016)
  • Author(s): Okumura M, Ozawa T, Taki H, Kishi H, Muraguchi A.
  • Organizer: 第45回日本免疫学会学術集会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県宜野湾市）
  • Year and Date: 2016-12-05
• [Presentation] Development of novel human anti-HLA-monoclonal antibodies for clinical applications using peripheral blood B cells derived from anti-HLA antibody-positive donors. (2016)
  • Author(s): Takamatsu H, Zaimoku Y, Ozawa T, Kawai S, Tanaka H, Kishi H, Muraguchi A, Nakao S.
  • Organizer: 58th ASH Annual Meeting & Exposition
  • Place of Presentation: San Diego（米国）
  • Year and Date: 2016-12-03
  • Int'l Joint Research
• [Presentation] ISAAC法を用いた抗原特異的T細胞検出法の開発． (2016)
  • Author(s): 小澤龍彦，岸 裕幸，浜名 洋，田尻和人，呂 福蓮，村口 篤．
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-11-30
• [Presentation] 免疫細胞療法の最新動向と今後の展開． (2016)
  • Author(s): 神垣 隆，安元公正，岸 裕幸，下岡清美，富山 舞．
  • Organizer: 第22回国際個別化医療学会学術集会
  • Place of Presentation: 大崎ブライトコアホール（東京都品川区）
  • Year and Date: 2016-10-29
• [Presentation] 腫瘍浸潤リンパ球の単一細胞解析による腫瘍特異的TCRの同定および治療への応用（担癌マウスモデル）． (2016)
  • Author(s): 岸 裕幸，村口 篤．
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-10-06
• [Presentation] CT26腫瘍局所浸潤CD8+T細胞のTCRレパトア解析と認識エピトープの同定． (2016)
  • Author(s): 藤井啓介，宮原慶裕，村岡大輔，下岡清美，浜名 洋，岸 裕幸，珠玖 洋．
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-10-06
• [Presentation] 関節リウマチ患者由来モノクローナル抗CCP抗体の取得と抗原の解析． (2016)
  • Author(s): 岸 裕幸，津田玲奈，小澤龍彦，村口 篤．
  • Organizer: 第28回日本神経免疫学会学術集会
  • Place of Presentation: 長崎ブリックホール（長崎県長崎市）
  • Year and Date: 2016-09-29
  • Invited
• [Presentation] Rapid generation of monoclonal antibodies using lymphocyte-microarray chip. (2016)
  • Author(s): Ozawa T, Kishi H., and Muraguchi A.
  • Organizer: 第68回日本生物工学会大会国際シンポジウム
  • Place of Presentation: 富山国際会議場（富山県富山市）
  • Year and Date: 2016-09-28
  • Int'l Joint Research / Invited
• [Presentation] Cis-interaction of TCRs and antigen/MHC class I complexes on CD8+ T cells causes their activation. (2016)
  • Author(s): Kishi H, Jin A, Hamana H, Shitaoka K, Tajiri K, Kobayashi E, Kusano S, Yokoyama S, Ozawa T, Nagai T, Obata T, Hatakeyama S, Horii M, Hu Y, Zhang F, Muraguchi A.
  • Organizer: 2016 International Congress of Immunology
  • Place of Presentation: Melbourne（豪州）
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] A rapid and efficient single-cell manipulation method using microwell array chip (ISAAC) technology for screening antigen-specific cytokines-secreting T-cells. (2016)
  • Author(s): Ozawa T, Kishi H, Hamana H, Tajiri K, Lyu F, Muraguchi A.
  • Organizer: 2016 International Congress of Immunology
  • Place of Presentation: Melbourne（豪州）
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] A rapid and easy system for screening of antigen-specific TCRs. (2016)
  • Author(s): Hamana H, Kishi H, Shitaoka K, Piao X, Ozawa T, Muraguchi A.
  • Organizer: 2016 International Congress of Immunology
  • Place of Presentation: Melbourne（豪州）
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] Identification of tumor-specific T cell receptors of primary tumor-infiltrating lymphocytes (TILs) from B16F10 melanoma-bearing mice at single cell levels. (2016)
  • Author(s): Shitaoka K, Hamana H, Kishi H, Ozawa T, Muraguchi A.
  • Organizer: 2016 International Congress of Immunology
  • Place of Presentation: Melbourne（豪州）
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] Establishment of West Nile virus-neutralizing human monoclonal antibodies derived from the individuals vaccinated with inactivated Japanese encephalitis virus by ISAAC technology. (2016)
  • Author(s): Masaki H., Ozawa T., Takasaki T., Aoyama I., Yumisashi T., Konishi E., Kishi H., and Muraguchi A.
  • Organizer: 2016 International Congress of Immunology
  • Place of Presentation: Melbourne（豪州）
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] びまん性大細胞型B細胞リンパ腫における腫瘍浸潤CD8+Tリンパ球由来T細胞受容体および腫瘍微小環境の解析． (2016)
  • Author(s): 村橋睦了，浜名 洋，松本太一，鍋島一樹，猪狩洋介，佐々木秀法，原 周司，谷 憲三朗，岸 裕幸，高松 泰．
  • Organizer: 第14回日本臨床腫瘍学会学術集会
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
  • Year and Date: 2016-07-28
• [Presentation] B16F10メラノーマ浸潤リンパ球の単一細胞解析による，がん特異的T細胞の同定およびTCR遺伝子治療の試み． (2016)
  • Author(s): 下岡清美，浜名 洋，呂 福蓮，小澤龍彦，早川芳弘，岸 裕幸，村口 篤．
  • Organizer: 第20回日本がん免疫学会総会
  • Place of Presentation: 大阪国際交流センター（大阪府大阪市）
  • Year and Date: 2016-07-27
• [Presentation] CT26腫瘍局所浸潤CD8+T細胞のTCRレパトア解析と認識エピトープの同定． (2016)
  • Author(s): 藤井啓介，宮原慶裕，村岡大輔，下岡清美，浜名 洋，岸 裕幸，珠玖 洋．
  • Organizer: 第20回日本がん免疫学会総会
  • Place of Presentation: 大阪国際交流センター（大阪府大阪市）
  • Year and Date: 2016-07-27
• [Presentation] 次世代シーケンサーとsingle cell sortingを用いた同種抗原反応性T細胞の網羅的解析． (2016)
  • Author(s): 川瀬孝和，坂本 葵，樗木 錬，美山貴彦，柴田真志，田中清人，北浦一孝，大島久美，浜名 洋，岸 裕幸，葛島清隆，佐治博夫，鈴木隆二，一戸辰夫．
  • Organizer: 第20回日本がん免疫学会総会
  • Place of Presentation: 大阪国際交流センター（大阪府大阪市）
  • Year and Date: 2016-07-27
• [Presentation] Comprehensive T-Cell Receptor Repertoire Analysis Using Deep Sequencing and Single Cell Cloning Reveals Extreme Oligoclonality of Ex Vivo Expanded Cytomegalovirus-Reactive Cytotoxic T-Cells. (2015)
  • Author(s): Miyama T, Kawase T, Kitaura K, Chishaki R, Shibata M, Oshima K, Hamana H, Kishi H, Kuzushima K, Saji H, Suzuki R, Ichinohe T.
  • Organizer: The 57th ASH Annual Meeting and Exposition
  • Place of Presentation: Orlando（米国）
  • Year and Date: 2015-12-05
  • Int'l Joint Research
• [Presentation] ISAAC法を用いた関節リウマチ患者由来自己抗体の単離とその自己抗体が認識する新しい自己抗原の同定． (2015)
  • Author(s): 小澤龍彦，津田玲奈，浜名 洋，多喜博文，戸邉一之，杉山英二，岩本雅弘，井村穣二，岸 裕幸，村口 篤．
  • Organizer: 第38回日本分子生物学会年会・第88回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] 抗膜タンパク質ウサギ高親和性モノクローナル抗体のエピトープ配列を由来とする新規アフィニティタグの開発． (2015)
  • Author(s): 矢野智也，竹田浩之，小澤龍彦，村口 篤，澤崎達也．
  • Organizer: 第38回日本分子生物学会年会・第88回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] コムギ無細胞系を用いた膜タンパク質間トランス相互作用解析技術． (2015)
  • Author(s): 栄谷紘一，竹田浩之，小澤龍彦，村口 篤，澤崎達也．
  • Organizer: 第38回日本分子生物学会年会・第88回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] ISAAC法による日本脳炎ワクチン被接種者末梢血単核球からのヒト抗ウエストナイルウイルス中和モノクローナル抗体の樹立． (2015)
  • Author(s): 正木秀幸，小澤龍彦，高崎智彦，青山幾子，弓指孝博，小西英二，岸 裕幸，村口 篤．
  • Organizer: 第63回日本ウイルス学会学術集会
  • Place of Presentation: 福岡国際会議場（福岡県福岡市）
  • Year and Date: 2015-11-22
• [Presentation] Assessment of T cell receptor function. (2015)
  • Author(s): Kishi H, Hamana H, Shitaoka K, Ozawa T, Muraguchi A.
  • Organizer: 第44回日本免疫学会学術集会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2015-11-18
• [Presentation] Development of an NFAT-luciferase reporter system for screening of antigen-specific TCRs using non-T cells. (2015)
  • Author(s): Hamana H, Kishi H, Shitaoka K, Piao X, Ozawa T, Muraguchi A.
  • Organizer: 第44回日本免疫学会学術集会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2015-11-18
• [Presentation] Identification of T-cell receptors of the CD8+ T cells in response to HLA-A24 restricted epitopes from hepatitis B virus. (2015)
  • Author(s): Tamai T, Kishi H, Shitaoka K, Hamana H, Ozawa T, Muraguchi A.
  • Organizer: 第44回日本免疫学会学術集会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2015-11-18
• [Presentation] Identification of tumor-specific T cell receptors from primary tumor-infiltrating lymphocytes (TILs) obtained from B16F10 melanoma-bearing mice. (2015)
  • Author(s): Shitaoka K, Kishi H, Hamana H, Ozawa T, Muraguchi A.
  • Organizer: 第44回日本免疫学会学術集会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2015-11-18
• [Presentation] Reactivity of a monoclonal ACPA obtained from RA patient with numerous citrullinated antigens. (2015)
  • Author(s): Ozawa T, Tsuda R, Hamana H, Taki H, Kishi H, Muraguchi A.
  • Organizer: 第44回日本免疫学会学術集会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2015-11-18
• [Presentation] TRAIL receptor-1 IgM antibodies strongly induced apoptosis in cancer cells. (2015)
  • Author(s): Piao X, Ozawa T, Kishi H, Shitaoka K, Hamana H, Muraguchi A.
  • Organizer: 第44回日本免疫学会学術集会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2015-11-18
• [Presentation] Isolation of monoclonal anti-TRIM21/Ro52 autoantibodies from collagen disease-patients with interstitial lung disease and their reactivity to the peptide library. (2015)
  • Author(s): Obi M, Ozawa T, Taki H, Kishi H, Muraguchi A.
  • Organizer: 第44回日本免疫学会学術集会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2015-11-18
• [Presentation] Establishment of West Nile virus-neutralizing human monoclonal antibodies derived from the individuals vaccinated with inactivated Japanese encephalitis virus by ISAAC technology. (2015)
  • Author(s): Masaki H, Ozawa T, Takasaki T, Kishi H, Muraguchi A.
  • Organizer: 第44回日本免疫学会学術集会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2015-11-18
• [Presentation] Single Cell Analysis of Antigen Receptors of Lymphocytes for Tumor Immunology and Immunotherapy. (2015)
  • Author(s): Kishi H.
  • Organizer: The Korean Association of Immunologists 2015 Fall Conference
  • Place of Presentation: Seoul（韓国）
  • Year and Date: 2015-11-12
  • Int'l Joint Research / Invited
• [Presentation] Comprehensive analysis of CMV-specific T-cell repertoire by deep sequencing and single cell cloning． (2015)
  • Author(s): 美山貴彦，川瀬孝和，北浦一孝，樗木 錬，柴田真志，大島久美，浜名 洋，岸 裕幸，葛島清隆，佐治博夫，鈴木隆二，一戸辰夫．
  • Organizer: 第77回日本血液学会学術集会
  • Place of Presentation: 石川県立音楽堂（石川県金沢市）
  • Year and Date: 2015-10-16
• [Presentation] プライマリー腫瘍浸潤リンパ球を用いた腫瘍特異的T細胞受容体同定の試み． (2015)
  • Author(s): 岸 裕幸，村口 篤．
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2015-10-08
• [Presentation] 悪性リンパ腫における腫瘍浸潤CD8+Tリンパ球の解析． (2015)
  • Author(s): 村橋睦了，岸 裕幸，松本太一，原 周司，村口 篤，田村和夫，谷憲三朗．
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2015-10-08
• [Presentation] 悪性リンパ腫における腫瘍浸潤CD8+Tリンパ球の解析． (2015)
  • Author(s): 岸 裕幸，村橋睦了，浜名 洋，松本太一，原 周司，猪狩洋介，石塚賢治，高松 泰，田村和夫，谷憲三朗，村口 篤．
  • Organizer: 第7回血液疾患免疫療法研究会学術集会
  • Place of Presentation: 東京大学伊藤謝恩ホール（東京都文京区）
  • Year and Date: 2015-09-26
• [Presentation] 悪性リンパ腫における腫瘍浸潤CD8+Tリンパ球の解析． (2015)
  • Author(s): 村橋睦了，松本太一，浜名 洋，勝屋弘雄，猪狩洋介，石塚賢治，原 周司，岸 裕幸，高松 泰，田村和夫．
  • Organizer: 第13回日本臨床腫瘍学会学術集会
  • Place of Presentation: ロイトン札幌（北海道札幌市）
  • Year and Date: 2015-07-16
• [Presentation] Distinct pattern of the binding of human monoclonal anti-cyclic citrullinated peptide antibody to joint synovium in between osteoarthritis and rheumatoid arthritis. (2015)
  • Author(s): Iwamoto M, Honne K, Hanai S, Sekiya H, Ozawa T, Kishi H, Muraguchi A, Minota S.
  • Organizer: 2015 Henry Kunkel Meeting
  • Place of Presentation: Annecy（フランス）
  • Year and Date: 2015-04-14
  • Int'l Joint Research
• [Presentation] Cis-interaction of TCR and antigen/MHC class I complexes on a CD8+ T cell. (2014)
  • Author(s): Kishi H, Hamana H, Tajiri K, Ozawa T, Muraguchi A.
  • Organizer: 第43回日本免疫学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2014-12-10 – 2014-12-12
• [Presentation] Rapid generation of rabbit monoclonal antibodies against G-protein coupled receptor and application of these antibodies. (2014)
  • Author(s): Ozawa T, Piao X, Kishi H, Muraguchi A.
  • Organizer: 第43回日本免疫学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2014-12-10 – 2014-12-12
• [Presentation] Development of simple and efficient method for amplification of TCR αβ cDNA from single human T cells. (2014)
  • Author(s): Hamana H, Kishi H, Ozawa T, Muraguchi A.
  • Organizer: 第43回日本免疫学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2014-12-10 – 2014-12-12
• [Presentation] Identification of T cell antigen by an artificial reporter-cell system. (2014)
  • Author(s): Piao X, Kobayashi E, Kishi H, Hamana H, Ozawa T, Muraguchi A.
  • Organizer: 第43回日本免疫学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2014-12-10 – 2014-12-12
• [Presentation] Establishment of West Nile virus-neutralizing human monoclonal antibodies derived from the individuals vaccinated with inactivated Japanese encephalitis virus by ISAAC technology. (2014)
  • Author(s): Masaki H, Ozawa T, Takasaki T, Kishi H, Muraguchi A.
  • Organizer: 第43回日本免疫学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2014-12-10 – 2014-12-12
• [Presentation] GPCR特異的ウサギモノクローナル抗体の迅速作製法の開発． (2014)
  • Author(s): 小澤龍彦，朴 秀虹，小林栄治，竹田浩之，澤崎達也，岸 裕幸，村口 篤．
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Functional features of antigen-specific T-cell receptors reflected clinical responses of α-fetoprotein-derived peptides vaccine treatment for advanced hepatocellular carcinoma. (2014)
  • Author(s): Nakagawa H, Mizukoshi E, Kobayashi E, Terashima T, Kitahara M, Iida N, Kishi H, Muraguchi A, Kaneko S.
  • Organizer: The 65th Annual Meeting of the American Association for the Study of Liver Diseases
  • Place of Presentation: Boston
  • Year and Date: 2014-11-07 – 2014-11-11
• [Presentation] 単一B細胞からの抗体の作製と評価． (2014)
  • Author(s): 村口 篤．
  • Organizer: 第87回日本生化学会大会フォーラム
  • Place of Presentation: 京都
  • Year and Date: 2014-10-15 – 2014-10-18
  • Invited
• [Presentation] メラノーマ患者および健常人PBMC中のCD4+ T細胞の単一細胞レベルでのレパートリー解析． (2014)
  • Author(s): 岸 裕幸，小林栄治，杉山大介，西川博嘉，坂口志文，村口 篤．
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] メラノーマ患者および健常人の末梢血CD4+ T細胞の単一細胞レベルでのレパートリー解析． (2014)
  • Author(s): 岸 裕幸，浜名 洋，小林栄治，杉山大介，西川博嘉，鬼塚志乃，坂口志文，村口 篤．
  • Organizer: 第6回血液疾患免疫療法研究会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2014-09-06
• [Presentation] がんペプチドワクチン療法におけるTCRレパートリーの経時的変化の検討． (2014)
  • Author(s): 中河秀俊，水腰英四郎，清家拓哉，山宮大典，米島 淳，稲田悠記，木田明彦，梶喜一郎，玉井利克，熊谷将史，寺島健志，北原征明，飯田宗穂，小林栄治，岸 裕幸，村口 篤，金子周一．
  • Organizer: 第18回日本がん免疫学会総会
  • Place of Presentation: 松山
  • Year and Date: 2014-07-30 – 2014-08-01
• [Presentation] Rapid generation of human monoclonal Abs and TCRs from single human lymphocyte: application for gene therapy of infectious diseases and cancer. (2014)
  • Author(s): Muraguchi A.
  • Organizer: The 6th China-Russia International Conference on Medicine
  • Place of Presentation: Harbin
  • Year and Date: 2014-07-01 – 2014-07-03
  • Invited