Application of cell cycle progression index to radiosensitivity evaluation and analysis of background factors on chromosome aberration

• Project/Area Number: 26293270
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: Hirosaki University
• Principal Investigator: Miura Tomisato 弘前大学, 保健学研究科, 准教授 (20261456)
• Co-Investigator(Kenkyū-buntansha): 阿部 悠 福島県立医科大学, 医学部, 助教 (00722472) ; 葛西 宏介 弘前大学, 保健学研究科, 講師 (50400148) ; 吉田 光明 弘前大学, 被ばく医療総合研究所, 教授 (60182789) ; 坂井 晃 福島県立医科大学, 医学部, 教授 (70284221) ; 中田 章史 北海道薬科大学, 薬学部, 講師 (70415420)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000); Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2016: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 細胞遺伝学的線量評価 / 緊急被ばく医療 / 染色体異常 / 放射線感受性 / メタボリックシンドローム / 放射線個人感受性 / 生活習慣病 / 二動原体染色体 / PCC法 / CPI / 放射線 / 線量評価 / 個人感受性 / バイオドシメトリー / 血液搬送 / 抗凝固剤 / 分裂頻度 / 糖尿病 / 動脈硬化症 / 被ばく医療

Outline of Final Research Achievements

In this study, we investigated the clinical laboratory index on chromosome aberration and examined the usefulness of cell cycle progression index (CPI) as a radiosensitivity evaluation tool. Ankle brachial pressure index (ABI), one of the indexes related to atherosclerosis, was correlated with the frequency of dicentric chromosomes induced by 3 Gy X-ray irradiation and CPI . In addition, a positive correlation was found between CPI and blood glucose level.
It is suggested that radiation induced chromosomal abnormalities increase in patients with arteriosclerosis and that CPI may be useful as a method for evaluating radiation individual susceptibility.

Research Products

• [Journal Article] Rapid isolation of murine primary hepatocytes for chromosomal analysis. (2017)
  • Author(s): Ariyoshi K, Fujishima Y, Miura T, Shang Y, Kakinuma S, Yoshiya S, Kasai K, Nakata A, Tachibana A, Yoshida MA
  • Journal Title: In Vitro Cell Dev Biol Anim Volume: in press Issue: 5 Pages: 474-478
  • DOI: 10.1007/s11626-017-0132-7
  • Peer Reviewed / Open Access
• [Journal Article] Analysis of chromosome translocation frequency after a single CT scan in adults (2016)
  • Author(s): Yu Abe, Tomisato Miura, Mitsuaki A. Yoshida, et al.
  • Journal Title: Journal of Radiation Research, Volume: 57 Issue: 3 Pages: 1-7
  • DOI: 10.1093/jrr/rrv090
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Induction of genomic instability and activation of autophagy in artificial human aneuploid cells. (2016)
  • Author(s): Ariyoshi K, Miura T, Kasai K, Fujishima Y, Oshimura M, Yoshida MA
  • Journal Title: Mutat Res Volume: 790 Pages: 19-30
  • DOI: 10.1016/j.mrfmmm.2016.06.001
  • Peer Reviewed
• [Journal Article] 細胞遺伝学的被ばく線量評価における抗凝固剤および血液保存温度の影響 (2016)
  • Author(s): 金浜朱希、藤嶋洋平、川森詩織、成田房恵、萩野繁樹、有吉健太郎、吉田光明、山田恭吾、真里谷靖、三浦 富智
  • Journal Title: 青森県臨床検査技師会誌 Volume: 41 Pages: 24-26
  • Acknowledgement Compliant
• [Journal Article] A novel parameter, cell-cycle progression index, for radiation dose absorbed estimation in the premature chromosome condensation assay (2014)
  • Author(s): Miura T, Nakata A, Kasai K, Nakano M, Abe Y, Tsushima E, Ossetrova NI, Yoshida MA, Blakely WF
  • Journal Title: radiat Prot Dosimetry Volume: 159 Issue: 1-4 Pages: 1-4
  • DOI: 10.1093/rpd/ncu126
  • Peer Reviewed
• [Journal Article] The Internet's role in a biodosimetric response to a radiation mass casualty event. (2014)
  • Author(s): Sugarman SL, Livingston GK, Stricklin DL, Abbott MG, Wilkins RC, Romm H, Oestreicher U, Yoshida MA, Miura T, Moquet JE, Di Giorgio M, Ferrarotto C, Gross GA, Christiansen ME, Hart CL, Christensen DM.
  • Journal Title: Health Phys Volume: 106
  • Peer Reviewed
• [Presentation] 低線量γ線照射による細胞遺伝学的線量評価用線量応答曲線の作製 (2016)
  • Author(s): 阿部悠, 三浦富智, 吉田光明, 氏家里紗, 黒須由美子, 柳亜希，津山尚宏, 川村文彦, 藤岡来実, 稲葉俊哉, 神谷研二，坂井晃
  • Organizer: 日本放射線影響学会第59回大会
  • Place of Presentation: JMSアステールプラザ, 広島県広島市
  • Year and Date: 2016-10-28
• [Presentation] バイオドシメトリーにおける抗凝固剤および血液保存温度の影響 (2016)
  • Author(s): 三浦富智、藤嶋洋平, 金浜朱希, 川森詩織, 成田房恵, 萩野繁樹, 有吉健太郎, 吉田光明, 葛西宏介, 山田恭吾, 真里谷靖
  • Organizer: 日本放射線影響学会第59回大会
  • Place of Presentation: ＪＭＳアステールプラザ, 広島県広島市
  • Year and Date: 2016-10-26
• [Presentation] 細胞遺伝学的被ばく線量評価における抗凝固剤および血液保存温度の影響 (2016)
  • Author(s): 金浜 朱希、藤嶋 洋平、川森 詩織、成田 房恵、萩野 繁樹、有吉 健太郎、吉田 光明、山田 恭吾、真里谷 靖、三浦 富智
  • Organizer: 第43回青森県医学検査学会
  • Place of Presentation: むつグランドホテル（青森県むつ市）
  • Year and Date: 2016-05-19
• [Presentation] Assessment of simulated partial body acute irradiation by cell-cycle progression index in the premature chromosome condensation assay (2015)
  • Author(s): Tomisato Miura, Yu Abe, Akifumi Nakata, Kosuke Kasai, Mitsuaki A. Yoshida
  • Organizer: The 15th International Congress of Radiation Research (ICRR 2015)
  • Place of Presentation: Kyoto International Conference Center（京都府京都市）
  • Year and Date: 2015-05-27
• [Presentation] ヒト末梢血ex vivo部分照射モデルにおけるCell-cycle Progression Indexの有用性評価 (2014)
  • Author(s): 三浦富智，阿部悠，中田章史，葛西宏介，吉田光明
  • Organizer: 日本放射線影響学会 第57回大会
  • Place of Presentation: かごしま県民交流センター(鹿児島県鹿児島市)
  • Year and Date: 2014-10-01 – 2014-10-03