| Project/Area Number |
26293272
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
粟飯原 輝人 筑波大学, 医学医療系, 准教授 (30268619)
沼尻 晴子 (橋井晴子) 筑波大学, 附属病院, 病院講師 (00712845)
大西 かよ子 筑波大学, 医学医療系, 講師 (60529832)
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| Co-Investigator(Renkei-kenkyūsha) |
TOYOHARA Jun 地方独立行政法人東京都健康長寿医療センター, 東京都健康長寿医療センター研究所, 研究副部長 (50425659)
THATANO Kentarou 筑波大学, 医学医療系, 准教授 (50228475)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2016: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2015: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | ホウ素中性子捕捉療法 / 膵臓癌 / 乳癌 / アポトーシス / BNCT / ＢＮＣＴ / 加速器 / 癌 / PET |
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| Outline of Final Research Achievements |
As a preclinical study of BNCT, we focused on collecting target data using X - rays. Cell viability of pancreatic cancer cells (BxPC-3) decreased dose-dependently. D10 was 1.28 Gy, and it was clarified that frequency of apoptosis is increased at the dose level. Expression analysis of cell death related protein by Western blotting method also increased the expression of Bax, but did not increase the expression of Bcl - 2, caspase 9, cleaved PARP. Similar results were obtained in animal experiments. In breast cancer cells (MDA-MB-231), the cell viability decreased in a dose-dependent manner, D10 was 3.92 Gy, and it was revealed that the dose increased frequency of apoptosis.
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