Radiation-induced injury in tissue-specific stem cells for evaluating non-cancer disease risks

• Project/Area Number: 26293279
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: Nagasaki University
• Principal Investigator: LI Tao-Sheng 長崎大学, 原爆後障害医療研究所, 教授 (50379997)
• Co-Investigator(Kenkyū-buntansha): 工藤 崇 長崎大学, 原爆後障害医療研究所, 教授 (20330300) ; 浦田 芳重 長崎大学, 原爆後障害医療研究所, 助教 (30185087) ; 後藤 信治 長崎大学, 原爆後障害医療研究所, 助教 (50186889) ; 小野 悠介 長崎大学, 原爆後障害医療研究所, 助教 (60601119)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000); Fiscal Year 2016: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2015: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2014: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
• Keywords: 幹細胞 / 放射線 / 非がん疾患リスク / 放射線傷害 / 心筋幹細胞 / 心臓幹細胞 / 再生修復機能

Outline of Final Research Achievements

In this study, we experimentally investigated radiation-induced injuries of cardiac stem cells, which might indirectly indicate future cardiovascular disease risks.
We found that the whole body exposure of healthy adult mouse to 3 Gy gamma ray significantly reduced the number, decreased telomerase activity, and increased DNA damage of cardiac stem cells, and also significantly impaired the self-regenerative potency of heart after injury. According to our data, radiation-induced changes on the c-kit expression, telomerase activity, and DNA damage of cardiac stem cells may server as parameters for indirectly evaluating future cardiovascular disease risks.

Research Products

• [Journal Article] Ionizing radiation impairs endogenous regeneration of infarcted heart: an in vivo 18F-FDG PET/CT and 99mTc-tetrofosmin SPECT/CT study in mice. (2017)
  • Author(s): Luo L, Nishi K, Urata Y, Yan C, Hasan AS, Goto S, Kudo T, Li ZL, Li TS
  • Journal Title: Radiation Research Volume: 187 Issue: 1 Pages: 89-97
  • DOI: 10.1667/rr14543.1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Dose-dependency and reversibility of radiation-induced injury in cardiac explant-derived cells of mice. (2017)
  • Author(s): Luo L, Yan C, Urata Y, Hasan AS, Goto S, Guo CY, Zhang S, Li TS
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 40959-40959
  • DOI: 10.1038/srep40959
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Radiation exposure decreases the quantity and quality of cardiac stem cells in mice. (2016)
  • Author(s): Luo L, Urata Y, Yan C, Hasan AS, Goto S, Guo CY, Tou FF, Xie Y, Li TS.
  • Journal Title: PLoS ONE Volume: 印刷中
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Time- and dose-dependent effects of total-body ionising radiation on muscle stem cells (2015)
  • Author(s): Masuda S, Hisamatsu T, Seko S, Urata Y, Goto S, Li TS & Ono Y
  • Journal Title: Physiol Rep Volume: 3(4) Issue: 4 Pages: e12377-e12377
  • DOI: 10.14814/phy2.12377
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Sensitivity and Dose Denpendency of Radiation-induced injury in hematopoietic Stem/Progenitor Cells in Mice (2015)
  • Author(s): Chang-Ying Guo, Lan Luo, Yoshishige Urata, Shinji Goto, Wen-Jing Huang, Syu Takamura, Fumiko Hayashi, Hanako Doi, Yuriko Kitajima, Yusuke Ono, Tomoo Ogi, and Tao-Sheng Li
  • Journal Title: Sci. Rep. Volume: 5 Issue: 1 Pages: 8055-8055
  • DOI: 10.1038/srep08055
  • Peer Reviewed / Open Access
• [Journal Article] The potential benefits of nicaraven to protect against radiation-induced injury in hematopoietic stem/progenitor cells with relative low dose exposures. (2014)
  • Author(s): Ali H, Galal O, Urata Y, Goto S, Guo CY, Luo L, Abdelrahim E, Ono Y, Mostafa E, Li TS.
  • Journal Title: Biochem Biophys Res Commun. Volume: 452 Issue: 3 Pages: 548-553
  • DOI: 10.1016/j.bbrc.2014.08.112
  • NAID: 120006986388
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Radiation-induced damages on cardiac stem cells and myocardial regeneration may indirectly indicate future cardiovascular disease risks (2016)
  • Author(s): Lan Luo1, Kodai Nishi2, Yoshishige Urata, Chen Yan, Shinji Goto, Takashi Kudo, Tao-Sheng Li
  • Organizer: 日本放射線影響学会第59回大会
  • Place of Presentation: JMSアステールプラザ（広島県・広島市）
  • Year and Date: 2016-10-26
• [Presentation] 放射線被ばくによる心血管疾患リスクに関する実験的科学評価 (2015)
  • Author(s): 羅蘭、浦田芳重、後藤信治、小野悠介、李桃生
  • Organizer: 第５６回原子爆弾後障害研究会
  • Place of Presentation: 広島国際会議場
  • Year and Date: 2015-06-06
• [Presentation] Radiation-induced damage of cardiac stem cells in quantity and quality (2015)
  • Author(s): Lan Luo, Yoshishige Urata, Shinji Goto, Yusuke Ono, Tao-Sheng Li
  • Organizer: ＩＣＲＲ２０１５
  • Place of Presentation: 京都国際会館
  • Year and Date: 2015-05-25 – 2015-05-29
• [Presentation] Radiation-induced damage of cardiac stem cells in quantity and quality (2015)
  • Author(s): Lan Luo, Yoshishige Urata, Shinji Goto, Yusuke Ono, Tao-Sheng Li
  • Organizer: ICRR2015 Kyoto Japan - 15th International Congress of Radiation Research
  • Place of Presentation: 京都府京都市国際会議場
  • Year and Date: 2015-05-25
  • Int'l Joint Research