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Identification of novel diagnostic markers for early detection of pancreatic cancer; application for a low-invasive fluid test

Research Project

Project/Area Number 26293285
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field General surgery
Research InstitutionNagoya University

Principal Investigator

KODERA Yasuhiro  名古屋大学, 医学(系)研究科(研究院), 教授 (10345879)

Co-Investigator(Kenkyū-buntansha) 神田 光郎  名古屋大学, 医学部附属病院, 助教 (00644668)
山田 豪  名古屋大学, 医学部附属病院, 病院講師 (30467287)
藤井 努  名古屋大学, 医学(系)研究科(研究院), 准教授 (60566967)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2016: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywords低侵襲スクリーニング法 / 膵腫瘍 / 次世代シーケンサー / 低侵襲スクリーング法 / 氏世代シーケンサー
Outline of Final Research Achievements

Previous studies indicated that pancreatic and duodenal juice samples are an excellent source of mutant DNA from the pancreas and have the potential to help in the risk stratification and surveillance of patients undergoing pancreatic screening. To identify novel genetic alterations specific to high grade precursors, we conducted an exome sequencing analysis. Mutations at NOTCH2, AFF3, EXT1, IL21R and CDH6 were frequently found in the high grade pancreatic intraepithelial neoplasias and ductal adenocarcinoma, but not normal ducts, indicating that these are promising markers for early detection of pancreatic cancer. We employed two highly-sensitive methods, Cast PCR and digital HRM, to detect less abundance of mutation and successfully detect 0.1% of mutant alleles. One or more of NOTCH2, AFF3, EXT1, IL21R and CDH6 mutations were found in the cystic fluids from high grade intraductal papillary mucinous neoplasms (IPMNs), whereas no mutations were seen in those from low grade IPMNs.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (2 results)

All 2017 2016

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (1 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] 膵腫瘍の遺伝子学的鑑別診断法の展開2017

    • Author(s)
      神田光郎
    • Journal Title

      肝胆膵

      Volume: 74 Pages: 175-181

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Presentation] Mutational status in precursor lesions of pancreatic cancer: Highlighting its diagnostic value2016

    • Author(s)
      Mitsuro Kanda, Tsutomu Fujii, Suguru Yamada, Machael Goggins, Yasuhiro Kodera
    • Organizer
      The Joint Conference of the 47th annual meeting of JPS, the 20th meeting of IAP, and the 6th meeting of AOPA
    • Place of Presentation
      Sendai International Center (宮城県・仙台市青葉区)
    • Year and Date
      2016-08-04
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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