| Project/Area Number |
26293299
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | International University of Health and Welfare (2016)
Chiba University (2014-2015) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
高野 重紹 千葉大学, 医学部附属病院, 助教 (20436380)
久保木 知 千葉大学, 医学部附属病院, 助教 (50571410)
吉富 秀幸 千葉大学, 医学部附属病院, 講師 (60375631)
加藤 厚 千葉大学, 医学(系)研究科(研究院), 講師 (70344984)
清水 宏明 千葉大学, 医学(系)研究科(研究院), 准教授 (80272318)
大塚 将之 千葉大学, 医学(系)研究科(研究院), 講師 (90334185)
賀川 真吾 千葉大学, 医学部附属病院, 助教 (90507302)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥12,220,000 (Direct Cost: ¥9,400,000、Indirect Cost: ¥2,820,000)
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| Keywords | 癌 / 外科 / プロテオーム |
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| Outline of Final Research Achievements |
We aimed to elucidate the key molecules involved in pancreatic ductal adenocarcinoma (PDAC) progression using proteomics approaches. First, we performed two-dimensional electrophoresis to identify the proteins overexpressed in PDAC tissues. Cofilin-1 were identified and verified as a candidate protein commonly upregulated in PDAC tissues. In immunohistochemistry, samples were divided into two groups based on the level of cofilin-1 expression, the High expression group showed significantly higher incidence of hematogenous dissemination in relapse forms of patients than did the Low expression group. After we established a detection system for immune-complex (IC) of cofilin-1 in sera, we observed that cofilin-1 IC levels in PDAC patients were significantly higher than those in healthy volunteers and patients with pancreatitis. Notably, those levels showed a stepwise increase during PDAC progression. These results suggest that cofilin-1 IC in sera is a potential serum biomarker for PDAC.
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