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Elucidation of non-invasive biomarker for early dtection of colorectal cancer

Research Project

Project/Area Number 26293301
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Digestive surgery
Research InstitutionMie University

Principal Investigator

Kusunoki Masato  三重大学, 医学系研究科, 教授 (50192026)

Co-Investigator(Kenkyū-buntansha) 問山 裕二  三重大学, 医学部附属病院, 准教授 (00422824)
井上 靖浩  三重大学, 医学系研究科, 客員准教授 (20324535)
毛利 靖彦  三重大学, 医学系研究科, 客員准教授 (70345974)
沖上 正人  三重大学, 医学系研究科, リサーチアソシエイト (90722596)
Project Period (FY) 2014-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2014: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Keywords大腸癌 / 早期診断 / バイオマーカー / 非侵襲的バイオマーカー / miRNA / エキソソーム / 診断 / microRNA / 早期診断マーカー / 大腸腺腫 / 血清マーカー / microRNAs / トランスレーショナルリサーチ / 小腸大腸肛門外科学
Outline of Final Research Achievements

The aim of this study was to identify non-invasive diagnostic biomarker for colorectal cancer. Array-based comprehensive analysis demonstrated that miR-1290 was one of candidate for diagnostic marker in CRC patients, and we validated diagnostic potential of serum miR-1290 using serum specimens from patients with adenoma and CRC. Surprisingly, serum miR-1290 could identify patients with adenoma or CRC with high accuracy (AUC=0.83, 0.718, respectively).

Academic Significance and Societal Importance of the Research Achievements

本研究結果を用いることで、大腸癌ならびにその前駆病変を早期に診断・同定しうる可能性が考慮された。

Report

(6 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (32 results)

All 2019 2018 2017 2016 2015 2014

All Journal Article (17 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 17 results,  Open Access: 7 results,  Acknowledgement Compliant: 1 results) Presentation (15 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Lymphocyte-C-reactive Protein Ratio as Promising New Marker for Predicting Surgical and Oncological Outcomes in Colorectal Cancer.2019

    • Author(s)
      Okugawa Y, Toiyama Y, Yamamoto A, Shigemori T, Ide S, Kitajima T, Fujikawa H, Yasuda H, Hiro J, Yoshiyama S, Yokoe T, Saigusa S, Tanaka K, Shirai Y, Kobayashi M, Ohi M, Araki T, McMillan DC, Miki C, Goel A, Kusunoki M
    • Journal Title

      Ann Surg. 2019 Feb 16.

      Volume: Feb Issue: 2 Pages: 16-16

    • DOI

      10.1097/sla.0000000000003239

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Clinical Implications of Pretreatment: Lymphocyte-to-Monocyte Ratio in Patients With Rectal Cancer Receiving Preoperative Chemoradiotherapy.2019

    • Author(s)
      Yamamoto A, Toiyama Y, Okugawa Y, Oki S, Ide S, Saigusa S, Araki T, Kusunoki M.
    • Journal Title

      Dis Colon Rectum.

      Volume: 62(2) Issue: 2 Pages: 171-180

    • DOI

      10.1097/dcr.0000000000001245

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] AZIN1 RNA editing confers cancer stemness and enhances oncogenic potential in colorectal cancer2018

    • Author(s)
      Shigeyasu Kunitoshi、Okugawa Yoshinaga、Toden Shusuke、Miyoshi Jinsei、Toiyama Yuji、Nagasaka Takeshi、Takahashi Naoki、Kusunoki Masato、Takayama Tetsuji、Yamada Yasuhide、Fujiwara Toshiyoshi、Chen Leilei、Goel Ajay
    • Journal Title

      JCI Insight

      Volume: 3 Issue: 12 Pages: 99976-99976

    • DOI

      10.1172/jci.insight.99976

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Prognostic impact of sarcopenia and its correlation with circulating miR-21 in colorectal cancer patients.2018

    • Author(s)
      Okugawa Y, Yao L, Toiyama Y, Yamamoto A, Shigemori T, Yin C, Omura Y, Ide S, Kitajima T, Shimura T, Fujikawa H, Yasuda H, Hiro J, Yoshiyama S, Kobayashi M, Tanaka K, Inoue Y, Araki T, Miki C, Kusunoki M.
    • Journal Title

      Oncol Rep.

      Volume: 39 Pages: 1555-1564

    • DOI

      10.3892/or.2018.6270

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] MiR-139-5p as a novel serum biomarker for recurrence and metastasis in colorectal cancer.2017

    • Author(s)
      Miyoshi J, Toden S, Yoshida K, Toiyama Y, Alberts SR, Kusunoki M, Sinicrope FA, Goel A.
    • Journal Title

      Sci Rep.

      Volume: - Issue: 1 Pages: 43393-43393

    • DOI

      10.1038/srep43393

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] CCAT1 and CCAT2 long noncoding RNAs, located within the 8q.24.21 ‘gene desert’, serve as important prognostic biomarkers in colorectal cancer2017

    • Author(s)
      Ozawa T, Matsuyama T, Toiyama Y, Takahashi N, Ishikawa T, Uetake H, Yamada Y, Kusunoki M, Calin G, Goel A.
    • Journal Title

      Annals of Oncology.

      Volume: 28 Issue: 8 Pages: 1882-1888

    • DOI

      10.1093/annonc/mdx248

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A Panel of Methylated MicroRNA Biomarkers for Identifying High-Risk Patients With Ulcerative Colitis-Associated Colorectal Cancer.2017

    • Author(s)
      Toiyama Y, Okugawa Y, Tanaka K, Araki T, Uchida K, Hishida A, Uchino M, Ikeuchi H, Hirota S, Kusunoki M, Boland CR, Goel A.
    • Journal Title

      Gastroenterology.

      Volume: 153 Issue: 6 Pages: 1634-1646

    • DOI

      10.1053/j.gastro.2017.08.037

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Successful identification of a predictive biomarker for lymph node metastasis in colorectal cancer using a proteomic approach.2017

    • Author(s)
      Mori K, Toiyama Y, Otake K, Ide S, Imaoka H, Okigami M, Okugawa Y, Fujikawa H, Saigusa S, Hiro J, Kobayashi M, Ohi M, Tanaka K, Inoue Y, Kobayashi Y, Mohri Y, Kobayashi I, Goel A, Kusunoki M.
    • Journal Title

      Oncotarget.

      Volume: 8 Issue: 63 Pages: 106935-106947

    • DOI

      10.18632/oncotarget.22149

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Circulating microRNA-1290 as a novel diagnostic and prognostic biomarker in human colorectal cancer2016

    • Author(s)
      Imaoka H, Toiyama Y, Fujikawa H, Hiro J, Saigusa S, Tanaka K, Inoue Y, Mohri Y, Mori T, Kato T, Toden S, Goel A, Kusunoki M.
    • Journal Title

      Ann Oncol.

      Volume: 27(10) Issue: 10 Pages: 1879-1886

    • DOI

      10.1093/annonc/mdw279

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] MicroRNA-7 Is Associated with Malignant Potential and Poor Prognosis in Human Colorectal Cancer.2016

    • Author(s)
      Nagano Y, Toiyama Y, Okugawa Y, Imaoka H, Fujikawa H, Yasuda H, Yoshiyama S, Hiro J, Kobayashi M, Ohi M, Araki T, Inoue Y, Mohri Y, Kusunoki M.
    • Journal Title

      Anticancer Res.

      Volume: 36(12) Pages: 6521-6526

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diagnostic Potential of Cell-Free and Exosomal MicroRNAs in the Identification of Patients with High-Risk Colorectal Adenomas.2016

    • Author(s)
      Uratani R, Toiyama Y, Kitajima T, Kawamura M, Hiro J, Kobayashi M, Tanaka K, Inoue Y, Mohri Y, Mori T, Kato T, Goel A, Kusunoki M.
    • Journal Title

      PLoS One.

      Volume: 11(10) Issue: 10 Pages: e0160722-e0160722

    • DOI

      10.1371/journal.pone.0160722

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] miRNA-503 Promotes Tumor Progression and Is Associated with Early Recurrence and Poor Prognosis in Human Colorectal Cancer.2016

    • Author(s)
      Noguchi T, Toiyama Y, Kitajima T, Imaoka H, Hiro J, Saigusa S, Tanaka K, Inoue Y, Mohri Y, Toden S, Kusunoki M
    • Journal Title

      Oncology.

      Volume: 90(4) Issue: 4 Pages: 221-231

    • DOI

      10.1159/000444493

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Circulating cell-free microRNAs as biomarkers for colorectal cancer.2016

    • Author(s)
      Toiyama Y, Tanaka K, Inoue Y, Mohri Y, Kusunoki M.
    • Journal Title

      Surg Today.

      Volume: 46 Pages: 13-24

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] SIGNIFICANCE OF NONCODING RNAS IN COLORECTAL CANCER: REVIEW2015

    • Author(s)
      Toiyama Y, Kusunoki M.
    • Journal Title

      日本外科学会雑誌

      Volume: 116 Pages: 360-365

    • NAID

      110010006639

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Serum miR-200c is a novel prognostic and metastasis-predictive biomarker in patients with colorectal cancer.2014

    • Author(s)
      Toiyama Y, Hur K, Tanaka K, Inoue Y, Kusunoki M, Boland CR, Goel A.
    • Journal Title

      Ann Surg.

      Volume: 259 Issue: 4 Pages: 735-743

    • DOI

      10.1097/sla.0b013e3182a6909d

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An update on microRNAs as colorectal cancer biomarkers: where are we and what's next?2014

    • Author(s)
      Okugawa Y, Toiyama Y, Goel A.
    • Journal Title

      Expert Rev Mol Diagn.

      Volume: 14 Issue: 8 Pages: 999-1021

    • DOI

      10.1586/14737159.2014.946907

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNA methylation and microRNA biomarkers for noninvasive detection of gastric and colorectal cancer.2014

    • Author(s)
      Toiyama Y, Okugawa Y, Goel A.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 455 Pages: 43-57

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] 大腸腺腫患者における非侵襲的診断マーカーとしての血清およびエクソソームmicro RNA の比較検討.2017

    • Author(s)
      北嶋貴仁、問山裕二、志村匡信、今岡裕基、沖上正人、安田裕美、奥川喜永、大北喜基、吉山繁幸、廣純一郎、三枝晋、井上幹大、小林美奈子、大井正貴、荒木俊光、田中光司、 井上靖浩、内田恵一、毛利靖彦、楠 正人
    • Organizer
      第87回大腸癌研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Diagnostic potential of serum and exosomal microRNAs for patients with pre-malignant lesions of colorectal cancer:Detection of adenoma.2017

    • Author(s)
      Uratani R,Toiyama Y,Kitajima T,Fujikawa H,Hiro J,Kobayashi M,Tanaka K,Inoue Y,Mohri Y,Mori T,Kato T,Goel A,Kusunoki M
    • Organizer
      AACR Annual Meeting , Washington,D.C., USA, April 4,2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 消化管悪性腫瘍におけるBDNF/TrkB axisの腹膜播種進展における機序解明と,治療標的としての可能性.2017

    • Author(s)
      奥川喜永、問山裕二、楠 正人
    • Organizer
      第15回日本消化器外科学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] The clinical significance of epigenetic microRNA-137 silencing in patients with ulcerative colitis.2017

    • Author(s)
      Toiyama Y, Okugawa Y , Hur K ,Tanaka K, Araki T, Uchida K , Uchino M , Ikeuchi H , Hirota S , Kusunoki M ,Boland CR, Goel A
    • Organizer
      AACR Annual Meeting , Washington,D.C., USA, April 4,2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Preoperative evaluation of heat shock protein 47 expression to identify patients with colorectal cancer with lymph node metastasis and poor prognosis.2017

    • Author(s)
      Mori K, Toiyama Y, Okugawa Y, Ichikawa T, Nagano Y, Oki S, Imaoka H, Okigami M, Fujikawa H, Hiro J, Saigusa S, Kobayashi M, Inoue Y, Mohri Y, Kusunoki M
    • Organizer
      2017 Gastrointestinal Cancers Symposium San Francisco, California, USA, Jan 21, 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 大腸癌組織におけるmicroRNA-7発現レベルと臨床病理学的意義2016

    • Author(s)
      長野 由佳, 問山 裕二, 今岡 裕基, 藤川 裕之, 廣 純一郎, 小林 美奈子, 田中 光司, 井上 靖浩, 毛利 靖彦, 楠 正人
    • Organizer
      日本消化器外科学会総会 71回
    • Place of Presentation
      アスティ徳島(徳島県、徳島市)
    • Year and Date
      2016-07-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] 大腸癌早期診断および予後マーカーとしてのcirculating microRNA-12902016

    • Author(s)
      今岡 裕基, 問山 裕二, 藤川 裕之, 三枝 晋, 廣 純一郎, 小林 美奈子, 井上 靖浩, 田中 光司, 毛利 靖彦, 楠 正人
    • Organizer
      日本消化器外科学会総会 71回
    • Place of Presentation
      アスティ徳島(徳島県、徳島市)
    • Year and Date
      2016-07-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] 大腸癌におけるmicroRNA-1290発現の機能的ならびに臨床病理学的意義2016

    • Author(s)
      今岡 裕基, 問山 裕二, 長野 由佳, 浦谷 亮, 森 浩一郎, 沖 哲, 藤川 裕之, 三枝 晋, 廣 純一郎, 小林 美奈子, 大井 正貴, 荒木 俊光, 田中 光司, 井上 靖浩, 毛利 靖彦, 楠 正人
    • Organizer
      日本外科学会定期学術集会 116回
    • Place of Presentation
      大阪国際会議場(大阪府、大阪市)
    • Year and Date
      2016-04-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] 大腸腺腫患者の非侵襲的診断マーカー 血清およびエクソソームmicro RNAの比較検討から2016

    • Author(s)
      浦谷 亮, 問山 裕二, 北嶋 貴仁, 森 浩一郎, 沖 哲, 今岡 裕基, 藤川 裕之, 廣 純一郎, 三枝 晋, 小林 美奈子, 大井 正貴, 荒木 俊光, 田中 光司, 井上 靖浩, 毛利 靖彦, 森 孝郎, 加藤 俊夫, 楠 正人
    • Organizer
      日本外科学会定期学術集会抄録集 116回
    • Place of Presentation
      大阪国際会議場(大阪府、大阪市)
    • Year and Date
      2016-04-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] 下部消化管 大腸癌における血清miR-203の臨床病理学的意義2016

    • Author(s)
      問山 裕二, 浦谷 亮, 沖 哲, 川村 幹雄, 藤川 裕之, 奥川 喜永, 廣 純一郎, 三枝 晋, 小林 美奈子, 荒木 俊光, 大井 正貴, 田中 光司, 井上 靖浩, 毛利 靖彦, 楠 正人
    • Organizer
      日本外科学会定期学術集会抄録集 116回
    • Place of Presentation
      大阪国際会議場(大阪府、大阪市)
    • Year and Date
      2016-04-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] 臨床外科に役立つ診断と治療のイノベーション 大腸癌バイオマーカーとしてのmicroRNAs2015

    • Author(s)
      問山 裕二(三重大学 消化管・小児外科), 井上 靖浩, 田中 光司, 浦谷 亮, 森 浩一郎, 今岡 裕基, 志村 匡信, 藤川 裕之, 三枝 晋, 廣 純一郎, 小林 美奈子, 大井 正貴, 荒木 俊光, 毛利 靖彦, 楠 正人
    • Organizer
      日本臨床外科学会総会
    • Place of Presentation
      福岡国際会議場[福岡県・福岡市)
    • Year and Date
      2015-11-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] 大腸がんバイオマーカー 大腸癌における診断および予後予測マーカーとしての血清miR-1290発現の検討2015

    • Author(s)
      今岡 裕基(三重大学 大学院医学系研究科), 問山 裕二, 浦谷 亮, 森 浩一郎, 志村 匡信, 藤川 裕之, 三枝 晋, 廣 純一郎, 小林 美奈子, 大井 正貴, 荒木 俊光, 田中 光司, 井上 靖浩, 毛利 靖彦, 楠 正人
    • Organizer
      日本癌治療学会学術集会
    • Place of Presentation
      国立京都国際会館(京都府・京都市)
    • Year and Date
      2015-10-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] 大腸がんバイオマーカー 大腸癌患者における転移ならびに予後診断マーカーとしての転移巣由来血清microRNA-2032015

    • Author(s)
      問山 裕二(三重大学 大学院医学系研究科消化管・小児外科), 今岡 裕基, 井出 正造, 志村 匡信, 安田 裕美, 藤川 裕之, 廣 純一郎, 三枝 晋, 小林 美奈子, 荒木 俊光, 大井 正貴, 田中 光司, 井上 靖浩, 毛利 靖彦, 楠 正人
    • Organizer
      日本癌治療学会学術集会
    • Place of Presentation
      国立京都国際会館(京都府・京都市)
    • Year and Date
      2015-10-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] 大腸癌治療におけるバイオマーカー 早期大腸癌診断に向けての大腸腺腫患者血清microRNAs発現の検討2014

    • Author(s)
      問山 裕二、北嶋 貴仁, 今岡 裕基, 近藤 哲, 井出 正造, 川村 幹雄, 川本 文, 奥川 喜永, 廣 純一郎, 三枝 晋, 小林 美奈子, 大井 正貴, 田中 光司, 井上 靖浩, 毛利 靖彦, 楠 正人
    • Organizer
      日本大腸肛門病学会
    • Place of Presentation
      横浜ベイホテル東急
    • Year and Date
      2014-11-07 – 2014-11-08
    • Related Report
      2014 Annual Research Report
  • [Presentation] 大腸癌転移、予後診断マーカーとしての血清マイクロRNA2014

    • Author(s)
      問山 裕二、井上 靖浩, 沖上 正人, 川村 幹雄, 川本 文, 奥川 喜永, 三枝 晋, 廣 純一郎, 田中 光司, 毛利 靖彦, Boland C. Richard, Goel Ajay, 楠 正人
    • Organizer
      日本癌学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2020-03-30  

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