Investigation and clinical application of mechanism for growth of minimal cancer cells in gastrointestinal cancer

• Project/Area Number: 26293306
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kagoshima University
• Principal Investigator: Natsugoe Shoji 鹿児島大学, 医歯学域医学系, 教授 (70237577)
• Co-Investigator(Kenkyū-buntansha): 盛 真一郎 鹿児島大学, 医歯学域医学部・歯学部附属病院, 助教 (00620519) ; 奥村 浩 鹿児島大学, 医歯(薬)学総合研究科, 客員研究員 (10398282) ; 中条 哲浩 鹿児島大学, 医歯学域医学部・歯学部附属病院, 助教 (20404486) ; 内門 泰斗 鹿児島大学, 医歯学域医学部・歯学部附属病院, 特例准教授 (30464465) ; 喜島 祐子 鹿児島大学, 医歯学域医学部・歯学部附属病院, 講師 (60381175) ; 上之園 芳一 鹿児島大学, 医歯(薬)学総合研究科, 特任准教授 (60398279) ; 石神 純也 鹿児島大学, 医歯(薬)学総合研究科, 客員研究員 (90325803)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000); Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
• Keywords: 微量癌細胞 / 消化器癌 / リンパ節 / 血中遊離癌細胞 / 血中遊離脂肪細胞 / 肝転移 / 化学療法 / 化学放射線療法

Outline of Final Research Achievements

A total of 89 gastric cancer and 511 nodal metastasis were immunohistochemically examined by E-cadherin, N-cadherin and Snail. N-cadherin and Snail were important markers to predict tumor stage and prognosis in gastric cancer. In esophageal cancer cases which were complete response by preoperative chemo-radiation therapy, 30% of patients had a minimal residual disease by cytokeratin staining. At baseline analysis, CTCs were detected in 25 patients (27.8%). Overall survival was significantly shorter in patients with than without CTCs. Patients with a change in CTC status from positive to negative had a good prognosis as well as patients without baseline CTCs.

Research Products

• [Journal Article] Clinical significance of altering epithelial-mesenchymal transition in metastatic lymph nodes of gastric cancer. (2017)
  • Author(s): Okubo K, Uenosono Y, Arigami T, Yanagita S, Matsushita D, Kijima T, Amatatsu M, Uchikado Y, Kijima Y, Maemura K, Natsugoe S.
  • Journal Title: Gastric Cancer Volume: － Issue: 5 Pages: 802-810
  • DOI: 10.1007/s10120-017-0705-x
  • NAID: 40021337917
  • Peer Reviewed
• [Journal Article] Clinical impact of circulating tumor cells and therapy response in pancreatic cancer. (2017)
  • Author(s): Okubo K, Uenosono Y, Arigami T, Mataki Y, Matsushita D, Yanagita S, Kurahara H, Sakoda M, Kijima Y, Maemura K, Natsugoe S.
  • Journal Title: Eur J Surg Oncol. Volume: － Issue: 6 Pages: 1050-1055
  • DOI: 10.1016/j.ejso.2017.01.241
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Filamin C promotes lymphatic invasion and lymphatic metastasis and increases cell motility by regulating Rho GTPase in esophageal squamous cell carcinoma. (2017)
  • Author(s): Tanabe K, Shinsato Y, Furukawa T, Kita Y, Hatanaka K, Minami K, Kawahara K, Yamamoto M, Baba K, Mori S, Uchikado Y, Maemura K, Tanimoto A, Natsugoe S.
  • Journal Title: Oncotarget. Volume: 8 Issue: 4 Pages: 6353-6363
  • DOI: 10.18632/oncotarget.14087
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The clinical usefulness of the intraoperative detection of sentinel lymph node metastases by a rapid RT-PCR system in patients with gastric cancer. (2016)
  • Author(s): Yanagita S, Uenosono Y, Arigami T, Daisuke M, Okubo K, Kijima T, Arima H, Hirata M, Haraguchi N, Hagihara T, Nishizono Y, Ishigami S, Natsugoe S.
  • Journal Title: Cancer. Volume: 122 Issue: 3 Pages: 386-392
  • DOI: 10.1002/cncr.29740
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Ｉｍｐａｃｔ ｏｆ ｐ53 ａｎｄ ＰＤＧＦＲ－β expression on metastasis and prognosis of patients with pancreatic cancer (2016)
  • Author(s): Ｈｉｒｏｓｈｉ Ｋｕｒａｈａｒａ
  • Journal Title: Ｗｏｒｌｄ Ｊｏｕｒｎａｌ ｏｆ Ｓｕｒｇｅｒｙ Volume: Ｅｐｕｂ ａｈｅａｄ ｏｆ ｐｒｉｎｔ Issue: 8 Pages: 1977-1984
  • DOI: 10.1007/s00268-016-3477-2
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Clinical Significance of Circulating Tumor Cells in Peripheral Blood of Patients with Esophageal Squamous Cell Carcinoma. (2015)
  • Author(s): Matsushita D, Uenosono Y, Arigami T, Yanagita S, Nishizono Y, Hagihara T, Hirata M, Haraguchi N, Arima H, Kijima Y, Kurahara H, Maemura K, Okumura H, Ishigami S, Natsugoe S
  • Journal Title: Ann Surg Oncol Volume: 22 Issue: 11 Pages: 3674-3680
  • DOI: 10.1245/s10434-015-4392-8
  • Peer Reviewed
• [Journal Article] Human leukocyte antigen (HLA)-E and HLA-F expression in gastric cancer. (2015)
  • Author(s): Ishigami S, Arigami T, Okumura H, Uchikado Y, Kita Y, Kurahara H, Maemura K, Kijima Y, Ishihara Y, Sasaki K, Uenosono Y, Natsugoe S.
  • Journal Title: Anticancer Res Volume: 35 Pages: 2279-2295
  • Peer Reviewed
• [Journal Article] Analysis of the Fibrinogen and Neutrophil-Lymphocyte Ratio in Esophageal Squamous Cell Carcinoma: A Promising Blood Marker of Tumor Progression and Prognosis. (2015)
  • Author(s): Arigami T, Okumura H, Matsumoto M, Uchikado Y, Uenosono Y, Kita Y, Owaki T, Mori S, Kurahara H, Kijima Y, Ishigami S, Natsugoe S
  • Journal Title: Medicine Volume: 94 Issue: 42 Pages: 1702-1702
  • DOI: 10.1097/md.0000000000001702
  • Peer Reviewed
• [Journal Article] 下咽頭癌と食道癌の重複癌に対する治療戦略 －食道癌の治療－ (2014)
  • Author(s): 夏越祥次，内門泰斗，奥村 浩
  • Journal Title: 耳鼻と臨床 Volume: 60 Pages: 3-8
  • NAID: 130005118740
  • Peer Reviewed
• [Journal Article] The usefulness of neoadjuvant chemoradiation therapy for locally advanced esophageal cancer with multiple lymph-node metastases． (2014)
  • Author(s): Okumura H，Uchikado Y，Omoto I，Kita Y，Sasaki K，Arigami T，Uenosono Y，Matsushita D，Hiraki Y，Owaki T，Ishigami S，Natsugoe S．
  • Journal Title: Ann Surg Oncol． Volume: 21 Pages: 2845-284
  • Peer Reviewed
• [Journal Article] Biomarkers for predicting the response of esophageal squamous cell carcinoma to neoadjuvant chemoradiation therapy． (2014)
  • Author(s): Okumura H，Uchikado Y，Setoyama T，Matsumoto M，Owaki T，Ishigami S，Natsugoe S．
  • Journal Title: Surgery Today Volume: 44 Pages: 421-428
  • Peer Reviewed
• [Journal Article] Clinical and biological impact of cyclin-dependent kinase subunit 2 (CKS2) in esophageal squamous cell carcinoma． (2014)
  • Author(s): Kita Y，Nishizono Y，Okumura H，Uchikado Y，Sasaki K，Matsumoto M，Setoyama T，Tanoue K，Omoto I，Mori S，Owaki T，Ishigami S，Nakagawa H，Tanaka F，Mimori K，Mori M，Natsugoe S．
  • Journal Title: Oncology Rep Volume: 31 Pages: 1986-1992
  • Peer Reviewed
• [Presentation] 切除不能・再発胃癌患者における血中循環癌細胞のHER2発現からみた化学 療法効果予測 (2016)
  • Author(s): 上之園芳一,有上貴明,柳田茂寛,大久保啓史,貴島 孝,松下大輔,奥村 浩,石神純也,帆北修一,夏越祥次
  • Organizer: 第24回日本消化器関連学会週間（JDDW）
  • Place of Presentation: 神戸コンベンションセンター（兵庫県神戸市）
  • Year and Date: 2016-11-05
• [Presentation] Laparoscopic and endoscopic cooperative surgery （LECS） with sentinel node dissection for early gastric cancer. (2016)
  • Author(s): Shoji Natsugoe
  • Organizer: Korea International Gastric Cancer Week 2016
  • Place of Presentation: ソウル（大韓民国）
  • Year and Date: 2016-04-22
  • Int'l Joint Research / Invited
• [Presentation] Overview of multimodal treatment for esophageal cancer. (2016)
  • Author(s): 夏越祥次
  • Organizer: 第116回日本外科学会定期学術集会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2016-04-15
  • Invited
• [Presentation] 切除不能・再発胃癌における化学療法中の血中循環癌細胞の変化とHER2発現に関する検討. (2016)
  • Author(s): 上之園芳一,有上貴明,柳田茂寛,大久保啓史,貴島 孝,松下大輔,奥村 浩,石神純也,帆北修一,夏越祥次
  • Organizer: 第116回日本外科学会定期学術集会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2016-04-15
• [Presentation] 胃癌における主病巣と転移巣でのEMTマーカー発現に関する検討 (2016)
  • Author(s): 大久保啓史，上之園芳一，有上貴明，柳田茂寛，松下大輔，貴島 孝，夏越祥次
  • Organizer: 第116回日本外科学会定期学術集会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2016-04-15
• [Presentation] 消化器外科領域の臨床研究ー微小転移診断と臨床応用ー (2015)
  • Author(s): 夏越祥次
  • Organizer: 第15回日本外科学会臨床研究セミナー
  • Place of Presentation: 福岡サンパレス（福岡県福岡市）
  • Year and Date: 2015-11-28
  • Invited
• [Presentation] 食道癌の治療 (2015)
  • Author(s): 夏越祥次
  • Organizer: 第53回日本癌治療学会学術集会
  • Place of Presentation: 国立京都国際会館（京都府京都市）
  • Year and Date: 2015-10-29
  • Invited
• [Presentation] 上部消化器癌のリンパ節微小転移 (2015)
  • Author(s): 夏越祥次
  • Organizer: 第24回がん転移学会学術集会
  • Place of Presentation: シティプラザ大阪（大阪府大阪市）
  • Year and Date: 2015-07-23
• [Presentation] Sentinel Node Navigation Surgery is Clinically Useful in Early Gastric Cancer (2015)
  • Author(s): 3.Natsugoe S, Uenosono Y, Arigami T, Yanagita S, Okubo K, Kijima T, Matsushita D, Ishigami S, Hokit S
  • Organizer: 11th International Gastric Cancer Congress
  • Place of Presentation: Sao Paulo, Brazil
  • Year and Date: 2015-06-03
  • Int'l Joint Research
• [Presentation] Various treatment of EGJ cancer (2015)
  • Author(s): Shoji Natsugoe
  • Organizer: 第20回中日消化器外科会議
  • Place of Presentation: Shanghai, China
  • Year and Date: 2015-05-30
  • Int'l Joint Research / Invited
• [Presentation] Sentinel node navigation surgery (2014)
  • Author(s): Natsugoe S
  • Organizer: 14th World Congress of the International Society of the Esophagus
  • Place of Presentation: Vancouver，Canada
  • Year and Date: 2014-09-22 – 2014-09-24
  • Invited
• [Presentation] Sentinel node navigation surgery in esophageal cancer (2014)
  • Author(s): Natsugoe S，Uenosono Y，Matsushita D，Arigami T，Yanagita S，Hagihara T，Omoto I，Uchikado Y，Okumura H，Ishigami S．
  • Organizer: 14th World Congress of the International Society of the Esophagus
  • Place of Presentation: Vancouver，Canada
  • Year and Date: 2014-09-22 – 2014-09-24
• [Presentation] 切除不能食道癌における Circulating Tumor Cells 発現の検討 (2014)
  • Author(s): 松下大輔，上之園芳一，有上貴明，柳田茂寛，平原徹志，大久保啓志，内門泰斗，奥村 浩，夏越祥次
  • Organizer: 第114回 日本外科学会定期学術集会 (優秀演題賞)
  • Place of Presentation: 京都（国立京都国際会館）
  • Year and Date: 2014-04-03 – 2014-04-05