Creation of a novel animal model of secondary lymphedema and development of the therapy
| Project/Area Number |
26293310
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cardiovascular surgery
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
Naoki Unno 浜松医科大学, 医学部, 准教授 (20291958)
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| Co-Investigator(Kenkyū-buntansha) |
山本 尚人 浜松医科大学, 医学部附属病院, 助教 (80402262)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2016: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
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| Keywords | リンパ浮腫 / 動物モデル / TGF-β / 線維化 / 筋線維芽細胞 / 治療 / Eicosapentaenoic acid / 実験モデル / 外科 / リンパ管学 / 脂肪細胞 / 線維芽細胞 / 蛍光リンパ管造影 / リンパ管造影 / 病態動物モデル |
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| Outline of Final Research Achievements |
The pathophysiology of secondary lymphedema remains unclear . Lack of an animal model has hindered investigations on the pathophysiology of the disease. We developed a novel animal model of secondary lymphedema using the rat hindlimb. Then, we identified that transforming growth factor (TGF)-β1 was produced by macrophages in the acute phase and by fibroblasts in the late phase of the disease. Further, TGF-β1 was observed to differentiate fibroblasts to myofibroblasts, accelerating collagen synthesis to result in fibrosis. Eicosapentaenoic acid (EPA) inhibited myofibroblasts producing TGF-β1 in skin samples harvested from the hindlimbs of our animal models, as well as from the legs of patients with secondary lymphedema. Moreover, daily administration of EPA effectively inhibited fibrosis in our rat model via suppression of TGF-β1. We showed EPA inhibits fibrosis in secondary lymphedema via suppression of myofibroblasts producing TGF-β1.
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Report
(4 results)
Research Products
(45 results)
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[Journal Article] Adipocyte in vascular wall can induce the rupture of abdominal aortic aneurysm.2016
Author(s)
Kugo H, Zaima N, Tanaka H, Mouri Y, Yanagimoto K, Hayamizu K, Hashimoto K, Sasaki T, Sano M, Yata T, Urano T, Setou M, Unno N, Moriyama T.
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Journal Title
Sci Rep.
Volume: 6
Issue: 1
Pages: 31268-31268
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Hemodynamic Analysis of Endoleaks After Endovascular Abdominal Aortic Aneurysm Repair by Using 4-Dimensional Flow-Sensitive Magnetic Resonance Imaging2016
Author(s)
Sakata M, Takehara Y, Katahashi K, Sano M, Inuzuka K, Yamamoto N, Sugiyama M, Sakahara H, Wakayama T, Alley MT, Konno H, Unno N
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Journal Title
Circulation Journal
Volume: 80
Issue: 8
Pages: 1715-1725
DOI
NAID
ISSN
1346-9843, 1347-4820
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Imaging Mass Spectrometry Reveals a Unique Distribution of Triglycerides in the Abdominal Aortic Aneurysmal Wall.2015
Author(s)
Tanaka H, Zaima N, Sasaki T, Yamamoto N, Inuzuka K, Sano M, Saito T, Hayasaka T, Goto-Inoue N, Sato K, Kugo H, Moriyama T, Konno H, Setou M, Unno N.
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Journal Title
J Vasc Res
Volume: 52
Issue: 2
Pages: 127-135
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Lymphangiogenesis and angiogenesis in abdominal aortic aneurysm.2014
Author(s)
Sano M, Sasaki T, Hirakawa S, Sakabe J, Ogawa M, Baba S, Zaima N, Tanaka H, Inuzuka K, Yamamoto N, Setou M, Sato K, Konno H, Unno N*
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Journal Title
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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