Regenerative therapy for postoperative paraplegia after thoracic aortic surgery using bone marrow stem cell cultured in microgravity

• Project/Area Number: 26293312
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular surgery
• Research Institution: Hiroshima University
• Principal Investigator: Sueda Taijiro 広島大学, 医歯薬保健学研究院(医), 教授 (10162835)
• Co-Investigator(Kenkyū-buntansha): 弓削 類 広島大学, 医歯薬保健学研究院(保), 教授 (20263676)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000); Fiscal Year 2016: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2014: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
• Keywords: 胸腹部大動脈瘤 / 対麻痺 / 脊髄再生 / 骨髄間葉系幹細胞 / 微小重力培養 / 神経分化誘導 / 神経幹細胞 / 脊髄障害 / 間葉系幹細胞 / GFP / 胸部大動脈瘤 / 脊髄間葉系幹細胞 / 大動脈瘤手術 / 脊髄虚血 / 神経再生 / 脊髄再生治療

Outline of Final Research Achievements

Paraplegia is devastating complication after thoracoabdominal aortic surgery. Bone marrow stem cell was cultivated using the microgravited (10-3G) culture device (Gravite). Bone marrow stem cell can be cultivated under undifferentiated condition by microgravitated clture device. Rat paraplegia model was made using simple balloon occlusion of descending aorta for 30min. Control group (serine), simple cultivated bone marrow stem cells and microgravitated bone marrow stem cells were injected into rat paraplegia model via tail vein. Bone marrow degeneration can be treated by injection of cultivated bone marrow stem cells.

Research Products

• [Journal Article] A novel blood viscosity estimation based on pressure -flow characteristics of an oxygenator during cardiopulmonary bypass (2017)
  • Author(s): Okahara S, Soh Z, Miyamoto S, Takahahsi H, Takahashi S, Sueda T, Tsuji T
  • Journal Title: Artif Organs Volume: 41 Pages: 262-266
  • Peer Reviewed / Open Access
• [Journal Article] Blood viscosity monitoring during cardiopulmonary bypass based on pressure-flow chracteristics of a Newtonian fluid (2016)
  • Author(s): Okahara S, Zu Soh, Takahahsi S Sueda T, Tsuji T
  • Journal Title: Conf Proc IEEE Eng Med Biol Soc Volume: 759 Pages: 1197-1199
  • Peer Reviewed / Open Access
• [Journal Article] Intraoperative factors postoperative atrial fibrillation? Reply (2016)
  • Author(s): Takahashi S, Sueda T
  • Journal Title: Cir J Volume: Epub ahead Frb Pages: 8-8
  • Peer Reviewed / Open Access
• [Journal Article] Iliac artery stenting combined with open iliofemoral endarterectomy facilitated by a novel ultrasonic ablation instrument for diffuse iliofemoral occlusive disease (2016)
  • Author(s): Takahashi S, Taguchi T, Dohi Y, Sueda T
  • Journal Title: Ann Vasc Surg Volume: 31 Pages: 13-16
  • Peer Reviewed / Open Access
• [Journal Article] Preoperative tissue Doppler imaging-derived atrial conduction time predict postoperative atrail fibrillation in patients undergoing mitral valve surgery for mitral valve regurgitation (2016)
  • Author(s): Takahashi S, Katayama K, Watanabe M, Kodama H, Taguchi T, Kurosaki T, Imai K, Sueda T
  • Journal Title: Cir J Volume: 80 Pages: 101-109
  • Peer Reviewed / Open Access
• [Journal Article] Iliac artery stenting combined with open iliofemoral endoarterectomy facilitated by a novel ultrasonic ablation instrument for diffuse iliofemoral occlusive disease (2016)
  • Author(s): Takahahsi S, Taguchi T, Dohi Y, Sueda T
  • Journal Title: Ann Vas Surg Volume: 31 Pages: 13-16
  • Peer Reviewed / Open Access
• [Journal Article] The relationship between inflammation and neoangiogenesis of epicardial adipose tissue and coronary atherosclerosis based on computed tomography analysis (2015)
  • Author(s): Kitagawa T, Tamamoto H, Sentani K, Takahahsi S, Tsushima H, Senoo A, Yasui W, SuedaT
  • Journal Title: Atherosclerosis Volume: 243 Pages: 293-9
  • Peer Reviewed / Open Access
• [Journal Article] The impact of preoperative identification of the Adamkiewicz artery on descending and thoracoabdominal aortic repair (2015)
  • Author(s): Tanaka H, Ogino H, Minatoya K, Matsui Y, Higami T, Okabatashi H, Sakai Y, Aomi Y, Shiiya N, Sawa Y, Okota Y, Sueda T, Akashi H, Kuniyoshi Y, Katsumata T
  • Journal Title: J Thorac Cardiovasc Surg Volume: 151 Pages: 122-8
  • Peer Reviewed / Open Access
• [Journal Article] Efficacy of cardiopulmonary rehabilitation with adaptive servo-ventilation in patients undergoing off-pump coronary artery bypass grafting (2015)
  • Author(s): Tashiro N, Takahahsi S, Takasaki T, Katayama K, Taguchi T, Watanabe M, Kurosaki T, Imai K, Sueda T
  • Journal Title: Cir J Volume: 79 Pages: 1290-8
  • NAID: 130005073148
  • Peer Reviewed / Open Access
• [Journal Article] Postoperative atrial fibrillation after thoracic aortic surgery (2015)
  • Author(s): Arakawa M, Miyata H, Uchida N, Motomura N, Katayama A, Tamura K, Sueda T, Takamoto S
  • Journal Title: Asian Cardiovasc Thorac Ann Volume: 99 Pages: 103-108
  • Peer Reviewed / Open Access
• [Journal Article] A successful hybrid repair for vertebral arteriovenous fistula with extracranial vertebral artery aneurysm (2015)
  • Author(s): Takahashi S, Katayama K, Tatsugawa T, Sueda T
  • Journal Title: Ann Vas Surg Volume: 29 Pages: 5-8
  • Peer Reviewed / Open Access
• [Journal Article] Prestenotic and post stenotic multiple aneurysms with adult coactation (2014)
  • Author(s): Katayama K, Uchida N, Takasaki T, Sueda T
  • Journal Title: J Vasc surg Volume: 60 Pages: 234-235
  • Peer Reviewed / Open Access
• [Journal Article] Multiple factors predict the risk of spinal cord injury after the frozen elephant trunk technique for extended thoracic aortic disease. (2014)
  • Author(s): Katayama K, Uchida N, Katayama A, Takahashi S, Takasaki T, Kurosaki T, Imai K, Sueda T
  • Journal Title: Eur J Cardiothorac Surg Volume: 47 Issue: 4 Pages: 616-620
  • DOI: 10.1093/ejcts/ezu243
  • Peer Reviewed / Open Access
• [Journal Article] Delayed surgery for type A aortic dissection caused by blunt trauma (2014)
  • Author(s): Takahashi S, Uchida N, Takasaki T, Sueda T
  • Journal Title: Asian Cardiovasc Thorac Ann Volume: 23 Pages: 206-208
  • Peer Reviewed / Open Access
• [Journal Article] Transintercostal-evoked spinal cord potential in thoracic aortic surgery (2014)
  • Author(s): Takahashi S, Orihahsi K, Imai K, Mizukami T, Takasaki T, Sueda T
  • Journal Title: Ann Vasc Surg Volume: 28 Pages: 1775-1781
  • Peer Reviewed / Open Access
• [Journal Article] Trehalose protects against spinal cord ischemia in rabbits (2014)
  • Author(s): Takahashi S, Isaka M, Hamamoto M, Imai K, Orihahsi K, Sueda T
  • Journal Title: J Vasc Surg Volume: 60 Pages: 490-496
  • Peer Reviewed / Open Access
• [Journal Article] Inhibition of somatosensory-evoked cortical responses by a weak leading stimulus (2014)
  • Author(s): Nakagawa K, Inui K, Yuge L, Kakigi R
  • Journal Title: Neuroimage Volume: 101 Pages: 416-424
  • Peer Reviewed / Open Access
• [Journal Article] Sensory incongruence leading to hand disownership modulates somatosensory cortical processing (2014)
  • Author(s): Otsuru N, Hoshizume A, Nakashima D, Endo Y, Inui K, Kakigi R, Yuge L
  • Journal Title: Cortex Volume: 58 Pages: 1-8
• [Presentation] Removal of infected sten graft (2016)
  • Author(s): Sueda T
  • Organizer: Japan Endovascular Symposium
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2016-08-23
  • Invited
• [Presentation] Spinal cord injur after thoracic endovascular aortic stent grafting (2016)
  • Author(s): Sueda T
  • Organizer: American Association for Thoracic Surgery-Aortic Symposium-
  • Place of Presentation: New York, USA
  • Year and Date: 2016-05-12
  • Int'l Joint Research
• [Presentation] 胸部大動脈ステントグラフトのトラブル対処 (2015)
  • Author(s): 末田泰二郎
  • Organizer: 第10回日本endovascular symposium
  • Place of Presentation: 東京
  • Year and Date: 2015-08-27
  • Invited
• [Presentation] Endovascular repair for aortic arch pathology in elderly patients (2015)
  • Author(s): Taijiro Sueda
  • Organizer: 23th annual meeting of the Asian Society for Cardiovascular and Thoracic Surgery
  • Place of Presentation: Hong Kong
  • Year and Date: 2015-05-11
  • Int'l Joint Research
• [Book] 新・心臓血管外科テキスト (2016)
  • Author(s): 末田泰二郎、高橋信也
  • Publisher: 中外医学社
• [Book] 日常診療に活かす診療ガイドラインup-to-date2016-2017 (2016)
  • Author(s): 大毛宏喜、末田泰二郎
  • Publisher: メデイカルレビュー社