Establishment of diagnostic method and pathophysiology for idiopathic normal pressure hydrocephalus
| Project/Area Number |
26293326
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurosurgery
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| Research Institution | Juntendo University |
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Principal Investigator |
Arai Hajime 順天堂大学, 医学部, 教授 (70167229)
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| Co-Investigator(Kenkyū-buntansha) |
中島 円 順天堂大学, 医学部, 准教授 (50317450)
宮嶋 雅一 順天堂大学, 医学部, 准教授 (60200177)
菅野 秀宣 順天堂大学, 医学部, 准教授 (90265992)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥12,740,000 (Direct Cost: ¥9,800,000、Indirect Cost: ¥2,940,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
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| Keywords | 糖タンパク質 / 認知機能障害 / トランスジェニックマウス / パッチクランプ / 認知機能 / 特発性正常圧水頭症 / 加齢 / 髄液診断 / 脳脊髄液 / 神経毒性蛋白 / 認知障害 / 加齢性蛋白 / 興奮性シナプス後場電位 / 長期増強 / 認知症 / 動物モデル / 正常圧水頭症 / 長期増強現象 / LRG1 |
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| Outline of Final Research Achievements |
To investigate the relationship between LRG expression in the brain and memory impairment, we analyzed transgenic mice overexpressing LRG in the brain (Tg) focusing on hippocampus.
Immunostaining and western blotting revealed age-related increase of LRG expression in hippocampal neuron . Y-maze and Morris water maze test indicated spatial memory was retained in 24-week-old Tg, while deteriorated in 48-week-old compared to age-matched controls. Field excitatory postsynaptic potential declined in Tg compared to controls in 8, 24, and 48-week-old, obviously with age. Paired-pulse ratio decreased with age in Tg, while increased in controls. As a result, long-term potentiation was retained in 8 and 24-week-old Tg while diminished in 48-week-old comparing with age-matched controls. Electron-microscopic observation revealed decrease of synaptic vesicles and junctions in Tg. Hippocampal LRG overexpression contributes to synaptic dysfunction, which lead to memory impairment with advance of age.
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Report
(4 results)
Research Products
(71 results)
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[Journal Article] Neurite Orientation Dispersion and Imaging for Evaluation of Corticospinal Tract in Idiopathic Normal Pressure Hydrocephalus2017
Author(s)
Ryusuke Irie, Kohei Tsuruta, Masaaki Hori, Michimasa Suzuki, Koji Kamagata, Atsushi Nakanishi, Kouhei Kamiya, Madoka Nakajima, Masakazu Miyajima, Hajime Arai, Shigeki Aoki
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Journal Title
Jpn J Radiol
Volume: 35
Issue: 1
Pages: 25-30
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Nationwide epidemiologic survey of idiopathic normal pressure hydrocephalus (iNPH) in Japan: The Epidemiological and clinical characteristics2017
Author(s)
Nagato Kuriyama, Masakazu Miyajima, Madoka Nakajima, Michiko Kurosawa, Wakaba Fukushima, Yoshiyuki Watanabe, Etsuko Ozaki, Yoshio Hirota, Akiko Tamakoshi, Etsuro Mori, Takeo Kato, Takahiko Tokuda, Akinori Urae, Hajime Arai
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Journal Title
Brain and behavior
Volume: e00635
Issue: 3
Pages: 1-10
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Diffusion imaging of reversible and irreversible microstructural changes within the corticospinal tract in idiopathic normal pressure hydrocephalus.2017
Author(s)
Kouhei Kamiya, Masaaki Hori, Ryohei Irie, Masakazu Miyajima, Madoka Nakajima, Koji Kamagata, Kouhei Tsuruta, Asami Saito, Misaki Nakazawa, Yuichi Suzuki, Harushi Mori, Akira Kunimatsu, Hajime Arai
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Journal Title
NeuroImage: Clinical
Volume: 14
Pages: 663-671
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Prospective estimation of mean axon diameter and extra-axonal space of the posterior limb of the internal capsule in patients with idiopathic normal pressure hydrocephalus before and after a lumboperitoneal shunt by using q-space diffusion MRI.2015
Author(s)
Masaaki Hori, Kouhei Kamiya, Atsushi Nakanishi, Issei Fukunaga, Masakazu Miyajima, Madoka Nakajima, Michimasa Suzuki, Yuriko Suzuki, Ryosuke Irie, Koji Kamagata, Hajime Arai, Shigeki Aoki
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Journal Title
Europiｎ Radiology
Volume: E-pub
Issue: 9
Pages: 1-7
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Nation wide epidemiological features & treatment of patients with idiopathic normal pressure hydrocephalus in Japan2015
Author(s)
Madoka Nakajima, Masakazu Miyajima, Ikuko Ogino, Michiko Kurosawa, Nagato Kuriyama, Wakaba Fukushima, Etsuro Mori, Takeo Kato, Chihiro Akiba, Hidenori Sugano, Kostadin Karagiozov, Hajime Arai
Organizer
Hydrocephalus 2015
Place of Presentation
Canada, Banff, The Fairmont Banff Springs Hotel
Year and Date
2015-09-19
Related Report
Int'l Joint Research
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[Presentation] Panventriculomegaly (PaVM) with a wide foramen of Magendie and large cisterna magna: clinical features and congenital etiologies2015
Author(s)
H Kageyama, I Ogino, K Shimoji, M Nakajima, R Fukai, N Miyake, K Nishiyama, N Matsumoto, H Arai, M Miyajima:
Organizer
Hydrocephalus 2015
Place of Presentation
Canada, Banff, The Fairmont Banff Springs Hotel
Year and Date
2015-09-19
Related Report
Int'l Joint Research
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[Presentation] 全国疫学調査によるiNPH治療解析2014
Author(s)
中島 円, 宮嶋雅一, 黒沢美智子, 栗山長門, 福島若葉, 廣田良夫, 玉腰暁子, 森悦朗, 加藤丈夫, 浦江明憲, 新井 一
Organizer
厚生労働省班会議
Place of Presentation
東京
Year and Date
2014-11-22
Related Report
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