Fluorescence imaging for investigation and treatment of bone and soft tissue tumors

• Project/Area Number: 26293333
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Kanazawa University
• Principal Investigator: Tsuchiya Hiroyuki 金沢大学, 医学系, 教授 (40227434)
• Co-Investigator(Kenkyū-buntansha): 林 克洋 金沢大学, 大学病院, 助教 (80507054) ; 山本 憲男 金沢大学, 医薬保健学総合研究科, 教授 (90332668) ; 三輪 真嗣 金沢大学, 大学病院, 医員 (40753455) ; 木村 浩明 名古屋市立大学, 医学(系)研究科(研究院), 助教 (50608693)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000); Fiscal Year 2016: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2014: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
• Keywords: 骨軟部腫瘍 / 転移 / 新規治療 / 転移メカニズム / 肉腫 / GSK-3β / アポトーシス / 腫瘍増殖 / 骨軟部肉腫 / 蛍光蛋白 / イメージング / 骨 / 悪性腫瘍 / 蛍光 / 手術 / 骨肉腫 / 骨転移

Outline of Final Research Achievements

We demonstrate in vivo fluorescence imaging of bone-marrow micrometastasis of prostate cancer at the cellular level in nude mice using PC-3 human prostate cancer cells labeled with green fluorescent protein (GFP). PC-3-GFP cells were visualized by real-time fluorescence imaging, to traffic and grow in the bone marrow. Formation of bone marrow micrometastasis could be imaged at the single-cell level in live mice, using confocal microscopy.
We investigated the efficacies of fluorescence-guided surgery (FGS), genetically-modified auxotrophic strain of Salmonella typhimurium (A1-R), and glycogen synthase kinase-3β (GSK- 3β) inhibitors. FGS significantly reduced residual tumor as well as the recurrence of experimental prostate-cancer bone metastasis. A1-R significantly inhibited tumor growth and metastasis in soft tissue tumor model and bone metastasis model. Treatment with GSK-3β inhibitors attenuated the growth of orthotopic osteosarcoma in mice.

Research Products

• [Int'l Joint Research] University of California, San Diego(米国)
• [Journal Article] Patient-derived orthotopic xenograft (PDOX) mouse model of adult rhabdomyosarcoma invades and recurs after resection in contrast to the subcutaneous ectopic model (2017)
  • Author(s): Igarashi K, Kawaguchi K, Kiyuna T, Murakami T, Miwa S, Nelson SD, Dry SM, Li Y, Singh A, Kimura H, Hayashi K, Yamamoto N, Tsuchiya H, Eilber FC, Hoffman RM
  • Journal Title: Cell Cycle Volume: 16 Issue: 1 Pages: 91-94
  • DOI: 10.1080/15384101.2016.1252885
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Use of αv Integrin Linked to GFP to Image Molecular Dynamics in Trafficking Cancer-Cell Emboli (2017)
  • Author(s): Tome Y, Yano S, Sugimoto N, Mii S, Bouvet M, Tsuchiya H, Kanaya F, Hoffman RM
  • Journal Title: J Cell Biochem Volume: 118 Issue: 1 Pages: 26-30
  • DOI: 10.1002/jcb.25603
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Surgical management of proximal fibular tumors: A report of 12 cases (2016)
  • Author(s): Inatani H, Yamamoto N, Hayashi K, Kimura H, Takeuchi A, Miwa S, Higuchi T, Abe K, Taniguchi Y, Yamada S, Okamoto H, Otsuka T, Tsuchiya H
  • Journal Title: J Bone Oncol Volume: 5 Issue: 4 Pages: 163-166
  • DOI: 10.1016/j.jbo.2016.06.001
  • NAID: 120005982984
  • Peer Reviewed / Open Access
• [Journal Article] Clinical Outcome of Reconstruction Using Frozen Autograft for a Humeral Bone Tumor (2016)
  • Author(s): Kimura H, Yamamoto N, Shirai T, Nishida H, Hayashi K, Tanzawa Y, Takeuchi A, Miwa S, Tsuchiya H
  • Journal Title: Anticancer Res Volume: 36 Pages: 6631-6635
  • Peer Reviewed
• [Journal Article] The disintegrin echistatin in combination with doxorubicin targets high-metastatic human osteosarcoma overexpressing ανβ3 integrin in chick embryo and nude mouse models (2016)
  • Author(s): Tome Y, Kimura H, Sugimoto N, Tsuchiya H, Kanaya F, Bouvet M, Hoffman RM
  • Journal Title: Oncotarget Volume: 7 Issue: 52 Pages: 87031-87036
  • DOI: 10.18632/oncotarget.13497
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Efficacy of glycogen synthase kinase-3β targeting against osteosarcoma via activation of β-catenin. (2016)
  • Author(s): Shimozaki S, Minamoto T, et al.
  • Journal Title: Oncotarget Volume: 7 Issue: 47 Pages: 77038-51
  • DOI: 10.18632/oncotarget.12781
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Non-toxic Efficacy of the Combination of Caffeine and Valproic Acid on Human Osteosarcoma Cells In Vitro and in Orthotopic Nude-mouse Models (2016)
  • Author(s): Igarashi K, Yamamoto N, Hayashi K, Takeuchi A, Kimura H, Miwa S, Hoffman RM, Tsuchiya H
  • Journal Title: Anticancer Res Volume: 36 Pages: 4477-82
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Efficacy of the Combination of a PARP Inhibitor and UVC on Cancer Cells as Imaged by Focus Formation by the DNA Repair-related Protein 53BP1 Linked to Green Fluorescent Protein (2016)
  • Author(s): Tome Y, Uehara F, Miwa S, Yano S, Mii S, Efimova EV, Bouvet M, Kimura H, Tsuchiya H, Kanaya F, Hoffman RM
  • Journal Title: Anticancer Res Volume: 36 Pages: 3821-6
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Use of αv Integrin Linked to Green Fluorescent Protein in Osteosarcoma Cells and Confocal Microscopy to Image Molecular Dynamics During Lung Metastasis in Nude Mice (2016)
  • Author(s): Tome Y, Yano S, Sugimoto N, Mii S, Uehara F, Miwa S, Bouvet M, Tsuchiya H, Kanaya F, Hoffman RM
  • Journal Title: Anticancer Res Volume: 36 Pages: 3811-6
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Clinical outcome of recurrent giant cell tumor of the extremity in the era before molecular target therapy: the Japanese Musculoskeletal Oncology Group study (2016)
  • Author(s): Takeuchi A, Tsuchiya H, Ishii T, Nishida Y, Abe S, Matsumine A, Kawai A, Yoshimura K, Ueda T
  • Journal Title: BMC Musculoskelet Disord Volume: 17 Issue: 1 Pages: 306-306
  • DOI: 10.1186/s12891-016-1163-z
  • Peer Reviewed / Open Access
• [Journal Article] The impact of complete surgical resection of spinal metastases on the survival of patients with thyroid cancer (2016)
  • Author(s): Kato S, Murakami H, Demura S, Fujimaki Y, Yoshioka K, Yokogawa N, Tsuchiya H
  • Journal Title: Cancer Med Volume: 5 Issue: 9 Pages: 2343-9
  • DOI: 10.1002/cam4.823
  • Peer Reviewed / Open Access
• [Journal Article] Clinical relevance of peroxisome proliferator-activated receptor-gamma expression in myxoid liposarcoma (2016)
  • Author(s): Takeuchi A, Yamamoto N, Shirai T, Hayashi K, Miwa S, Munesue S, Yamamoto Y, Tsuchiya H
  • Journal Title: BMC Cancer Volume: 16 Issue: 1 Pages: 442-442
  • DOI: 10.1186/s12885-016-2524-6
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Disintegrin targeting of an αvβ3 integrin-over-expressing high-metastatic human osteosarcoma with echistatin inhibits cell proliferation, migration, invasion and adhesion in vitro (2016)
  • Author(s): Tome Y, Kimura H, Kiyuna T, Sugimoto N, Tsuchiya H, Kanaya F, Bouvet M, Hoffman RM
  • Journal Title: Oncotarget Volume: 7 Issue: 29 Pages: 46315-46320
  • DOI: 10.18632/oncotarget.10111
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Balancing Prolonged Survival with QoL Using Low-dose Pazopanib Maintenance: A Comparison with the PALETTE Study (2016)
  • Author(s): Abe K, Yamamoto N, Hayashi K, Takeuchi A, Miwa S, Inatani H, Higuchi T, Taniguchi Y, Tsuchiya H
  • Journal Title: Anticancer Res Volume: 36 Pages: 2893-7
  • Peer Reviewed
• [Journal Article] The outcomes of reconstruction using frozen autograft combined with iodine-coated implants for malignant bone tumors: compared with non-coated implants (2016)
  • Author(s): Shirai T, Tsuchiya H, Terauchi R, Tsuchida S, Mizoshiri N, Igarashi K, Miwa S, Takeuchi A, Kimura H, Hayashi K, Yamamoto N, Kubo T
  • Journal Title: Jpn J Clin Oncol Volume: 46 Issue: 8 Pages: 735-40
  • DOI: 10.1093/jjco/hyw065
  • NAID: 120005899104
  • Peer Reviewed
• [Journal Article] Tenosynovial giant cell tumors in unusual locations detected by positron emission tomography imaging confused with malignant tumors: report of two cases (2016)
  • Author(s): Takeuchi A, Yamamoto N, Hayashi K, Miwa S, Takahira M, Fukui K, Oikawa T, Tsuchiya H
  • Journal Title: BMC Musculoskelet Disord Volume: 17 Issue: 1 Pages: 180-180
  • DOI: 10.1186/s12891-016-1050-7
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Real-time in vivo confocal fluorescence imaging of prostate cancer bone-marrow micrometastasis development at the cellular level in nude mice (2016)
  • Author(s): Miwa S, Toneri M, Igarashi K, Yano S, Kimura H, Hayashi K, Yamamoto N, Tsuchiya H, Hoffman RM
  • Journal Title: J Cell Biochem Volume: 117 Issue: 11 Pages: 2533-2537
  • DOI: 10.1002/jcb.25545
  • Peer Reviewed / Open Access
• [Journal Article] Fluorescence-guided surgery of human prostate cancer experimental bone metastasis in nude mice using anti-CEA DyLight 650 for tumor illumination. (2016)
  • Author(s): Miwa S, De Magalhaes N, Toneri M, Zhang Y, Cao W, Bouvet M, Tsuchiya H, Hoffman RM.
  • Journal Title: Journal of Orthopedic Research Volume: 34 Issue: 4 Pages: 559-65
  • DOI: 10.1002/jor.22973
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Repeated total en bloc spondylectomy for spinal metastases at different sites in one patient. (2015)
  • Author(s): Sugita S, Murakami H, Demura S, Kato S, Yoshioka K, Yokogawa N, Tanaka S, Tsuchiya H.
  • Journal Title: Europearn Spine Journal Volume: 24 Issue: 10 Pages: 2196-200
  • DOI: 10.1007/s00586-015-4091-y
  • NAID: 120005647658
  • Peer Reviewed
• [Journal Article] Treatment of a Simple Bone Cyst Using a Cannulated Hydroxyapatite Pin. (2015)
  • Author(s): Shirai T, Tsuchiya H, Terauchi R, Tsuchida S, Mizoshiri N, Ikoma K, Fujiwara H, Miwa S, Kimura H, Takeuchi A, Hayashi K, Yamamoto N, Kubo T.
  • Journal Title: Medicine (Baltimore) Volume: 94 Issue: 25 Pages: e1027-e1027
  • DOI: 10.1097/md.0000000000001027
  • Peer Reviewed / Open Access
• [Journal Article] Intraperitoneal administration of tumor-targeting Salmonella typhimurium A1-R inhibits disseminated human ovarian cancer and extends survival in nude mice. (2015)
  • Author(s): Matsumoto Y, Miwa S, Zhang Y, Zhao M, Yano S, Uehara F, Yamamoto M, Hiroshima Y, Toneri M, Bouvet M, Matsubara H, Tsuchiya H, Hoffman RM.
  • Journal Title: Oncotarget Volume: 6 Pages: 11369-77
  • Peer Reviewed / Open Access
• [Journal Article] Heterogeneous cell-cycle behavior in response to UVB irradiation by a population of single cancer cells visualized by time-lapse FUCCI imaging. (2015)
  • Author(s): Miwa S, Yano S, Kimura H, Yamamoto M, Toneri M, Murakami T, Hayashi K, Yamamoto N, Fujiwara T, Tsuchiya H, Hoffman RM.
  • Journal Title: Cell Cycle Volume: 14 Issue: 12 Pages: 1932-1937
  • DOI: 10.1080/15384101.2015.1033598
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cell-cycle fate-monitoring distinguishes individual chemosensitive and chemoresistant cancer cells in drug-treated heterogeneous populations demonstrated by real-time FUCCI imaging. (2015)
  • Author(s): Miwa S, Yano S, Kimura H, Yamamoto M, Toneri M, Matsumoto Y, Uehara F, Hiroshima Y, Murakami T, Hayashi K, Yamamoto N, Bouvet M, Fujiwara T, Tsuchiya H, Hoffman RM.
  • Journal Title: Cell cycle Volume: 14 Issue: 4 Pages: 621-629
  • DOI: 10.4161/15384101.2014.991604
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Salvage method for unplanned excision of soft tissue sarcoma: long-term results of second-look surgery following radio-hyperthermo-chemotherapy. (2015)
  • Author(s): Hayashi K, Yamada S, Inatani H, Okamoto H, Takeuchi A, Nishida H, Yamamoto N, Tsuchiya H, Otsuka T.
  • Journal Title: Anticancer Research Volume: 35 Pages: 493-498
  • Peer Reviewed
• [Journal Article] Effectiveness of two novel anionic and cationic platinum complexes in the treatment of osteosarcoma. (2015)
  • Author(s): Igarashi K, Yamamoto N, Hayashi K, Takeuchi A, Miwa S, Odani A, Tsuchiya H.
  • Journal Title: Anticancer Agents Med Chem Volume: 15 Pages: 390-399
  • NAID: 120005647662
  • Peer Reviewed
• [Journal Article] Efficacy of triplet regimen antiemetic therapy for chemotherapy-induced nausea and vomiting (CINV) in bone and soft tissue sarcoma patients receiving highly emetogenic chemotherapy, and an efficacy comparison of single-shot palonosetron and consecutive-day granisetron for CINV in a randomized, single-blinded crossover study. (2015)
  • Author(s): Kimura H, Yamamoto N, Shirai T, Nishida H, Hayashi K, Tanzawa Y, Takeuchi A, Igarashi K, Inatani H, Shimozaki S, Kato T, Aoki Y, Higuchi T, Tsuchiya H.
  • Journal Title: Cancer Med Volume: 4 Issue: 3 Pages: 333-41
  • DOI: 10.1002/cam4.373
  • Peer Reviewed / Open Access
• [Journal Article] Surgical site infection after total en bloc spondylectomy: risk factors and the preventive new technology. (2015)
  • Author(s): Hayashi H, Murakami H, Demura S, Kato S, Yoshioka K, Shinmura K, Yokogawa N, Ishii T, Fang X, Shirai T, Tsuchiya H.
  • Journal Title: Spine J Volume: 15 Pages: 132-7
  • NAID: 120005537885
  • Peer Reviewed
• [Journal Article] Inhibition of spontaneous and experimental lung metastasis of soft-tissue sarcoma by tumor-targeting Salmonella typhimurium A1-R (2014)
  • Author(s): Miwa S, Zhang Y, Baek KE, Uehara F, Yano S, Yamamoto M, Hiroshima Y, Matsumoto Y, Kimura H, Hayashi K, Yamamoto N, Tsuchiya H, Bouvet M, Hoffman RM, Zhao M
  • Journal Title: Oncotarget Volume: 5 Pages: 12849-12861
  • Peer Reviewed / Open Access
• [Journal Article] Tumor-targeting Salmonella typhimurium A1-R prevents experimental human breast cancer bone metastasis in nude mice (2014)
  • Author(s): Miwa S, Yano S, Zhang Y, Matsumoto Y, Uehara F, Yamamoto M, Hiroshima Y, Kimura H, Hayashi K, Yamamoto N, Bouvet M, Tsuchiya H, Hoffman RM, Zhao M
  • Journal Title: Oncotarget Volume: 5 Pages: 7119-71125
  • Peer Reviewed / Open Access
• [Journal Article] Fluorescence-guided surgery improves outcome in an orthotopic osteosarcoma nude-mouse model (2014)
  • Author(s): Miwa S, Hiroshima Y, Yano S, Zhang Y, Matsumoto Y, Uehara F, Yamamoto M, Kimura H, Hayashi K, Bouvet M, Tsuchiya H, Hoffman RM
  • Journal Title: Journal of Orthopaedic Ressearch Volume: 32 Issue: 12 Pages: 1596-1601
  • DOI: 10.1002/jor.22706
  • Peer Reviewed
• [Journal Article] Fluorescence guided-surgery of prostate cancer bone metastasis (2014)
  • Author(s): Miwa S, Matsumoto Y, Hiroshima Y, Yano S, Uehara F, Yamamoto M, Zhang Y, Kimura H, Hayashi K, Yamamoto N, Bouvet M, Sugimoto N, Tsuchiya H, Hoffman RM
  • Journal Title: Journal of Surgical Research Volume: 192 Issue: 1 Pages: 124-133
  • DOI: 10.1016/j.jss.2014.05.049
  • Peer Reviewed
• [Journal Article] Late recurrence of osteosarcoma: a report of two cases. (2014)
  • Author(s): Igarashi K, Yamamoto N, Shirai T, Nishida H, Hayashi K, Tanzawa Y, Kimura H, Takeuchi A, Miwa S, Inatani H, Shimozaki S, Kato T, Tsuchiya H.
  • Journal Title: J Orthop Surg (Hong Kong) Volume: 22 Pages: 415-419
  • Peer Reviewed / Open Access
• [Journal Article] Motor function of the upper-extremity after transection of the second thoracic nerve root during total en bloc spondylectomy. (2014)
  • Author(s): Yokogawa N, Murakami H, Demura S, Kato S, Yoshioka K, Hayashi H, Ishii T, Fujii M, Igarashi T, Tsuchiya H.
  • Journal Title: PLoS One Volume: 9 Issue: 10 Pages: e109838-e109838
  • DOI: 10.1371/journal.pone.0109838
  • NAID: 120005518032
  • Peer Reviewed / Open Access
• [Journal Article] Outcomes and complications of reconstruction using tumor-bearing frozen autografts in patients with metastatic bone tumors. (2014)
  • Author(s): Miwa S, Takeuchi A, Shirai T, Yamamoto N, Hayashi K, Nishida H, Kimura H, Igarashi K, Tsuchiya H.
  • Journal Title: Anticancer Res Volume: 34 Pages: 5569-5577
  • Peer Reviewed
• [Journal Article] Factors that influence functional outcome after total or subtotal scapulectomy: Japanese Musculoskeletal Oncology Group (JMOG) study. (2014)
  • Author(s): Hayashi K, Iwata S, Ogose A, Kawai A, Ueda T, Otsuka T, Tsuchiya H.
  • Journal Title: PLoS One Volume: 9 Issue: 6 Pages: e100119-e100119
  • DOI: 10.1371/journal.pone.0100119
  • Peer Reviewed / Open Access
• [Journal Article] Antimicrobial megaprostheses supported with iodine. (2014)
  • Author(s): Shirai T, Tsuchiya H, Nishida H, Yamamoto N, Watanabe K, Nakase J, Terauchi R, Arai Y, Fujiwara H, Kubo T.
  • Journal Title: J Biomater Appl Volume: 29 Issue: 4 Pages: 617-23
  • DOI: 10.1177/0885328214539365
  • Peer Reviewed
• [Journal Article] Accurate leg length measurement in total hip arthroplasty: a comparison of computer navigation and a simple manual measurement device. (2014)
  • Author(s): Ogawa K, Kabata T, Maeda T, Kajino Y, Tsuchiya H.
  • Journal Title: Clin Orthop Surg Volume: 6 Issue: 2 Pages: 153-8
  • DOI: 10.4055/cios.2014.6.2.153
  • Peer Reviewed / Open Access
• [Journal Article] Perioperative complications of total en bloc spondylectomy: adverse effects of preoperative irradiation. (2014)
  • Author(s): Yokogawa N, Murakami H, Demura S, Kato S, Yoshioka K, Hayashi H, Ishii T, Igarashi T, Fang X, Tsuchiya H.
  • Journal Title: PLoS One Volume: 9 Issue: 6 Pages: e98797-e98797
  • DOI: 10.1371/journal.pone.0098797
  • Peer Reviewed / Open Access
• [Journal Article] Grafted tendon healing in femoral and tibial tunnels after anterior cruciate ligament reconstruction. (2014)
  • Author(s): Nakase J, Kitaoka K, Toratani T, Kosaka M, Ohashi Y, Tsuchiya H.
  • Journal Title: J Orthop Surg (Hong Kong) Volume: 22 Pages: 65-9
  • Peer Reviewed / Open Access
• [Journal Article] Uncultured autogenous adipose-derived regenerative cells promote bone formation during distraction osteogenesis in rats. (2014)
  • Author(s): Nomura I, Watanabe K, Matsubara H, Hayashi K, Sugimoto N, Tsuchiya H.
  • Journal Title: Clin Orthop Relat Res Volume: 472 Issue: 12 Pages: 3798-806
  • DOI: 10.1007/s11999-014-3608-8
  • Peer Reviewed
• [Journal Article] Prevention of pin tract infection with iodine-supported titanium pins. (2014)
  • Author(s): Shirai T, Watanabe K, Matsubara H, Nomura I, Fujiwara H, Arai Y, Ikoma K, Terauchi R, Kubo T, Tsuchiya H.
  • Journal Title: J Orthop Sci Volume: 19 Issue: 4 Pages: 598-602
  • DOI: 10.1007/s00776-014-0561-z
  • NAID: 120005435509
  • Peer Reviewed
• [Presentation] 骨軟部腫瘍における手術とリハビリテーション (2016)
  • Author(s): 土屋弘行
  • Organizer: 第11回日本リハビリテーション医学会専門医会学術集会
  • Place of Presentation: 金沢市
  • Year and Date: 2016-10-29
  • Invited
• [Presentation] Treatment guidelines for musculoskeletal sarcoma in Japan (2016)
  • Author(s): Tsuchiya H
  • Organizer: 第54回日本癌治療学会
  • Place of Presentation: 横浜市
  • Year and Date: 2016-10-20
  • Invited
• [Presentation] Correction of the deformity related to benign bone tumor (2016)
  • Author(s): Tsuchiya H
  • Organizer: 第60回韓国整形外科学会
  • Place of Presentation: インチョン，韓国
  • Year and Date: 2016-10-19
  • Invited
• [Presentation] Iodine-coated titanium plates, rods and ex fix pins (2016)
  • Author(s): Tsuchiya H
  • Organizer: 26th Annual Baltimore Deformity Course
  • Place of Presentation: Baltimore, USA
  • Year and Date: 2016-08-26
  • Int'l Joint Research
• [Presentation] External fixation techniques in tumor surgery (2016)
  • Author(s): Tsuchiya H
  • Organizer: 26th Annual Baltimore Deformity Course
  • Place of Presentation: Baltimore, USA
  • Year and Date: 2016-08-26
  • Int'l Joint Research
• [Presentation] 骨欠損に対する生物学的再建術ー未来に向けてー (2016)
  • Author(s): 土屋弘行
  • Organizer: 第89回日本整形外科学会学術総会
  • Place of Presentation: 横浜市
  • Year and Date: 2016-05-12
  • Invited
• [Presentation] Antimicrobial implant technology with iodine-coating (2016)
  • Author(s): Tsuchiya H
  • Organizer: X Congresso Brasileiro De Oncologia Ortopedica
  • Place of Presentation: Foz do Iguassu, Brazil
  • Year and Date: 2016-04-20
  • Int'l Joint Research / Invited
• [Presentation] Frozen autograft reconstruction (2016)
  • Author(s): Tsuchiya H
  • Organizer: X Congresso Brasileiro De Oncologia Ortopedica
  • Place of Presentation: Foz do Iguassu, Brazil
  • Year and Date: 2016-04-20
  • Int'l Joint Research / Invited
• [Presentation] Correction of tumor-related deformities (2016)
  • Author(s): Tsuchiya H
  • Organizer: X Congresso Brasileiro De Oncologia Ortopedica
  • Place of Presentation: Foz do Iguassu, Brazil
  • Year and Date: 2016-04-20
  • Int'l Joint Research / Invited
• [Presentation] Efficacy of fluorescence-guided surgery on primary human osteosarcoma. (2016)
  • Author(s): Miwa S, Uehara Fu, Kimura H, Hayashi K, Yamamoto N, Hoffman RM, Tsuchiya H.
  • Organizer: 2016 AAOS Annual Meeting
  • Place of Presentation: Orlando, Florida
  • Year and Date: 2016-03-01
  • Int'l Joint Research
• [Presentation] 難治例に対する四肢再建術の進歩－創外固定からインプラントまで－ (2016)
  • Author(s): 土屋弘行
  • Organizer: 第8回南北合同東京整形外科医療連携フォーラム
  • Place of Presentation: 東京
  • Invited
• [Presentation] 骨軟部腫瘍に対する患肢温存手術とリハビリテーション (2016)
  • Author(s): 土屋弘行
  • Organizer: 第53回日本リハビリテーション医学会学術集会
  • Place of Presentation: 京都市
  • Invited
• [Presentation] 整形外科医の夢・挑戦・実現（Dream, Dare and Do.）ー“かがやき”をはなてるか？ (2016)
  • Author(s): 土屋弘行
  • Organizer: 第５回JASA(Japan Association of Spine Surgeons with Ambition)
  • Place of Presentation: 金沢市
  • Invited
• [Presentation] Orthopaedic hardware modifications for osteomyelitis (2016)
  • Author(s): Tsuchiya H
  • Organizer: 26th Annual Baltimore Limb Deformity Pre-course Masters of Disaster: Managing Osteomyelitis in the 21st Century
  • Place of Presentation: Baltimore, USA
  • Int'l Joint Research
• [Presentation] Cierny-Mader Stage 3 tibial osteomyelitis (2016)
  • Author(s): Tsuchiya H
  • Organizer: 26th Annual Baltimore Limb Deformity Pre-course Masters of Disaster: Managing Osteomyelitis in the 21st Century
  • Place of Presentation: Baltimore, USA
  • Int'l Joint Research
• [Presentation] Case presentation-Femoral infected non-union (2016)
  • Author(s): Tsuchiya H
  • Organizer: 26th Annual Baltimore Limb Deformity Pre-course Masters of Disaster: Managing Osteomyelitis in the 21st Century
  • Place of Presentation: Baltimore, USA
  • Int'l Joint Research
• [Presentation] 金沢発整形外科医療の最前線 (2016)
  • Author(s): 土屋弘行
  • Organizer: 第158回神奈川整形外科災害外科研究会
  • Place of Presentation: 横浜市
  • Invited
• [Presentation] 難治例に対する四肢再建術の進歩 (2016)
  • Author(s): 土屋弘行
  • Organizer: 北海道大学第15回MOS会
  • Place of Presentation: 札幌市
  • Invited
• [Presentation] 四肢再建術の進歩－創外固定からインプラントまで－ (2016)
  • Author(s): 土屋弘行
  • Organizer: 第6回徳島運動器疾患の疼痛を考える会
  • Place of Presentation: 徳島市
  • Invited
• [Presentation] Biological reconstruction using liquid nitrogen treated tumor-bearing bone for malignant bone tumor (2015)
  • Author(s): N. Yamamoto, K. Hayashi, A. Takeuchi, S. Miwa, H. Inatani, T. Higuchi, Y. Aoki, K. Abe, A. Abdelaal, P. Suwanpramote, H. Tsuchiya
  • Organizer: 18th World Congress of International Society of Cryosurgery
  • Place of Presentation: Kanazawa
  • Year and Date: 2015-11-26
  • Int'l Joint Research
• [Presentation] A novel method to apply adipose-derived stem cells to bone and soft tissue defects after tumor excision. (2015)
  • Author(s): Hayashi K, Yamamoto N, Shirai T, Takeuchi A, Kimura H, Miwa S, Suganuma S, Nomura I, Inatani H, Aoki Y, Fang X, Tsuchiya H
  • Organizer: International Society of Limb Salvage & Musculoskeletal Tumor Society combined meeting
  • Place of Presentation: W Marriott Orlando Grande Lakes Orlando
  • Year and Date: 2015-10-06
  • Int'l Joint Research
• [Presentation] Development of a novel molecular-targeted therapy for osteosarcoma (2015)
  • Author(s): N. Yamamoto, S. Shimozaki, K. Hayashi, A. Takeuchi, S. Miwa, H. Inatani, Y. Aoki, T. Higuchi, K. Abe, T. Minamoto, H. Tsuchiya
  • Organizer: 2015 ISOLS/MSTS Combined Annual Meeting
  • Place of Presentation: Orlando, USA
  • Year and Date: 2015-10-06
  • Int'l Joint Research
• [Presentation] Reconstruction of malignant bone tumors using pedicle frozen autograft treated with liquid nitrogen (2014)
  • Author(s): Tsuchiya H
  • Organizer: XXVI SICOT Triennial World Congress
  • Place of Presentation: SulAmerica Convention Center （Rio de Janeiro, Brazil）
  • Year and Date: 2014-11-19 – 2014-11-22
• [Presentation] Advantage of antimicrobial iodine-coated implants for prevention and treatment of implant-associated orthopaedic infections (2014)
  • Author(s): Tuchiya H
  • Organizer: XXVI SICOT Triennial World Congress
  • Place of Presentation: SulAmerica Convention Center （Rio de Janeiro, Brazil）
  • Year and Date: 2014-11-19 – 2014-11-22
• [Presentation] Epiphyseal preservation and biological reconstruction for metaphyseal osteosarcoma (2014)
  • Author(s): Tsuchiya H, Matsubara H, Takeuchi A, Yamamoto N, Nishida H, Hayashi K, Aikawa T, Yoshida Y
  • Organizer: The 24th Annual Meeting of LLRS (ASAMI-North America)
  • Place of Presentation: Fairmont The Queen Elizabeth （Montreal, Canada）
  • Year and Date: 2014-07-25 – 2014-07-26
• [Presentation] 患肢温存手術の進歩—夢の途中 (2014)
  • Author(s): 土屋弘行
  • Organizer: 第87回日本整形外科学会学術総会
  • Place of Presentation: 神戸ポートピアホテル、神戸国際展示場、神戸国際会議場（兵庫県神戸市）
  • Year and Date: 2014-05-21 – 2014-05-25
• [Presentation] Fluorescence-guided surgery decreases residual tumor in primary osteosarcoma model (2014)
  • Author(s): Miwa S, Hiroshima Y, Yano S, Matsumoto Y, Uehara F, Yamamoto M, Kimura H, Hayashi K, Tsuchiya H, Hoffman RM
  • Organizer: 2014 American Society of Cancer Research (AACR) annual meeting
  • Place of Presentation: San Diego Convention Center （San Diego, USA）
  • Year and Date: 2014-04-05 – 2014-04-09
• [Presentation] The efficacy of tumor-targeting Salmonella typhimurium A1-R for bone metastasis (2014)
  • Author(s): Miwa S, Yano S, Zhang Y, Uehara F, Matsumoto Y, Hiroshima Y, Yamamoto M, Kimura H, Hayashi K, Tsuchiya H, Zhao M, Hoffman RM
  • Organizer: 2014 American Society of Cancer Research (AACR) annual meeting
  • Place of Presentation: San Diego Convention Center （San Diego, USA）
  • Year and Date: 2014-04-05 – 2014-04-09
• [Book] Management of juxtaphyseal malignant bone tumors around the knee joint: New concepts in limb-sparing surgery. In Pediatric Lower Limb Deformities-principles and techniques of management, Sabharwal S ed. (2016)
  • Author(s): Matsubara H & Tsuchiya H
  • Total Pages: 10
  • Publisher: Springer Cham Heidelberg, New York Dordrecht London