Clarification of the tumor microenvironment in the development of urothelial carcinoma using animal model

• Project/Area Number: 26293349
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: University of Tsukuba
• Principal Investigator: Nishiyama Hiroyuki 筑波大学, 医学医療系, 教授 (20324642)
• Co-Investigator(Kenkyū-buntansha): 宮崎 淳 国際医療福祉大学, 医学部, 主任教授 (10550246) ; 小島 崇宏 筑波大学, 医学医療系, 講師 (40626892) ; 常樂 晃 筑波大学, 医学医療系, 講師 (60436277) ; 河合 弘二 筑波大学, 医学医療系, 講師 (90272195) ; 高岡 栄一郎 筑波大学, 医学医療系, 講師 (50625340)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000); Fiscal Year 2017: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
• Keywords: モルモット膀胱癌 / 脂質免疫 / ミコール酸 / Mycobacterium bovis BCG / CD1b / Mincle / Trem2 / Mycobacterium bovie BCG / TDM / 尿路上皮癌 / 癌免疫 / モルモットモデル / ノックアウトマウス / 腫瘍微小環境 / 腫瘍免疫 / 発癌

Outline of Final Research Achievements

We tested that guinea pigs can have bladder cancer by orally ingesting bracken. As a result of HE staining and immunostaining of the tumor of the bladder in the guinea pigs, it was confirmed that the tumor were non-muscle invaseve bladdere tumora. Guinea pigs have the same CD1b as human for lipid immunity. CD1b is a receptor for mycolic acid and we believe that future use of this bladder cancer cell line will elucidate the involvement of lipid immunity and T cell immunity.

Research Products

• [Journal Article] A comparison of nephrotoxicity between patients with a solitary-functioning kidney and those with bilateral-functioning kidneys in cisplatin-based chemotherapy for advanced urothelial carcinoma: a Japanese retrospective multi-institutional study (2018)
  • Author(s): Inoue Takamitsu、Miyazaki Jun、Ichioka Daishi、Narita Shintaro、Kageyama Susumu、Sugimoto Mikio、Mitsuzuka Koji、Shiraishi Yusuke、Kinoshita Hidefumi、Wakeda Hironobu、Nomoto Takeshi、Kikuchi Eiji、Matsui Yoshiyuki、Fujie Keiko、Habuchi Tomonori、Nishiyama Hiroyuki
  • Journal Title: BMC cancer Volume: 18 Issue: 1 Pages: 290-290
  • DOI: 10.1186/s12885-018-4186-z
  • NAID: 120007134237
  • Peer Reviewed / Open Access
• [Journal Article] Potential use of prostate specific membrane antigen (PSMA) for detecting the tumor neovasculature of brain tumors by PET imaging with 89Zr-Df-IAB2M anti-PSMA minibody (2018)
  • Author(s): Matsuda Masahide、Ishikawa Eiichi、Yamamoto Tetsuya、Hatano Kentaro、Joraku Akira、Iizumi Yuichi、Masuda Yosuke、Nishiyama Hiroyuki、Matsumura Akira
  • Journal Title: Journal of Neuro-Oncology Volume: in print Issue: 3 Pages: 581-589
  • DOI: 10.1007/s11060-018-2825-5
  • Peer Reviewed
• [Journal Article] High prevalence of hypogonadism determined by serum free testosterone level in Japanese testicular cancer survivors (2018)
  • Author(s): Kurobe Masahiro、Kawai Koji、Suetomi Takahiro、Iwamoto Teruaki、Waku Natsui、Kawahara Takashi、Kojima Takahiro、Joraku Akira、Miyazaki Jun、Nishiyama Hiroyuki
  • Journal Title: International Journal of Urology Volume: in print Issue: 5 Pages: 457-462
  • DOI: 10.1111/iju.13537
  • Peer Reviewed
• [Journal Article] Involvement of TRPV3 and TRPM8 ion channel proteins in induction of mammalian cold-inducible proteins (2018)
  • Author(s): Fujita Takanori、Liu Yu、Higashitsuji Hiroaki、Itoh Katsuhiko、Shibasaki Koji、Fujita Jun、Nishiyama Hiroyuki
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 495 Issue: 1 Pages: 935-940
  • DOI: 10.1016/j.bbrc.2017.11.136
  • NAID: 120006460063
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A Phase III trial of a single early intravesical instillation of pirarubicin to prevent bladder recurrence after radical nephroureterectomy for upper tract urothelial carcinoma (JCOG1403, UTUC THP Phase III). (2018)
  • Author(s): Miyamoto K, Ito A, Wakabayashi M, Eba J, Arai Y, Nishiyama H, Sugimoto M, Yamashita R, Kakehi Y.
  • Journal Title: Japanese Journal of Clinical Oncology Volume: 48 Pages: 94-97
  • DOI: 10.1093/jjco/hyx158
  • Peer Reviewed
• [Journal Article] Asia Consensus Statement on NCCN Clinical Practice Guideline for bladder cancer (2017)
  • Author(s): Nishiyama Hiroyuki
  • Journal Title: Japanese Journal of Clinical Oncology Volume: 48 Issue: 1 Pages: 3-6
  • DOI: 10.1093/jjco/hyx130
  • Peer Reviewed
• [Journal Article] Possible risk of overestimation of renal function using cystatin C-based eGFR in testicular cancer survivors treated with cisplatin-based chemotherapy (2017)
  • Author(s): Ichioka Daishi、Kawai Koji、Tanaka Ken、Ishitsuka Ryutaro、Yoshino Takayuki、Kimura Tomokazu、Kandori Shuya、Kawahara Takashi、Kojima Takahiro、Usui Joichi、Yamagata Kunihiro、Miyazaki Jun、Nishiyama Hiroyuki
  • Journal Title: Clinical and Experimental Nephrology Volume: in print Issue: 3 Pages: 727-734
  • DOI: 10.1007/s10157-017-1474-x
  • Peer Reviewed
• [Journal Article] Guidelines for treatment of renal injury during cancer chemotherapy 2016 (2017)
  • Author(s): Horie Shigeo、Oya Mototsugu、Nangaku Masaomi、Yasuda Yoshinari、Komatsu Yasuhiro、Yanagita Motoko、Kitagawa Yuko、Kuwano Hiroyuki、Nishiyama Hiroyuki、Ishioka Chikashi、Takaishi Hiromasa、Shimodaira Hideki、Mogi Akira、Ando Yuichi、Matsumoto Koji、Kadowaki Daisuke、Muto Satoru
  • Journal Title: Clinical and Experimental Nephrology Volume: 22 Issue: 1 Pages: 210-244
  • DOI: 10.1007/s10157-017-1448-z
  • NAID: 120007134431
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Relationship between Semenogelins bound to human sperm and other semen parameters and pregnancy outcomes (2017)
  • Author(s): Yamasaki Kazumitsu、Yoshida Kaoru、Yoshiike Miki、Shimada Kazuhiko、Nishiyama Hiroyuki、Takamizawa Satoru、Yanagida Kaoru、Iwamoto Teruaki
  • Journal Title: Basic and Clinical Andrology Volume: 27 Issue: 1 Pages: 15-15
  • DOI: 10.1186/s12610-017-0059-6
  • NAID: 120007128294
  • Peer Reviewed / Open Access
• [Journal Article] Epidemiology of urothelial carcinoma (2017)
  • Author(s): Miyazaki Jun、Nishiyama Hiroyuki
  • Journal Title: International Journal of Urology Volume: 24 Issue: 10 Pages: 730-734
  • DOI: 10.1111/iju.13376
  • Peer Reviewed
• [Journal Article] TRPV4-dependent induction of a novel mammalian cold-inducible protein SRSF5 as well as CIRP and RBM3 (2017)
  • Author(s): Fujita Takanori、Higashitsuji Hiroaki、Higashitsuji Hisako、Liu Yu、Itoh Katsuhiko、Sakurai Toshiharu、Kojima Takahiro、Kandori Shuya、Nishiyama Hiroyuki、Fukumoto Motoi、Fukumoto Manabu、Shibasaki Koji、Fujita Jun
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 2295-2295
  • DOI: 10.1038/s41598-017-02473-x
  • NAID: 120007129048
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Impact of lymph node dissection on clinical outcomes during nephroureterectomy in patients with clinically node-negative upper urinary tract urothelial cancer: subanalysis of a multi-institutional nationwide case series of the Japanese Urological Association (2017)
  • Author(s): Inokuchi Junichi、Eto Masatoshi、Hara Tomohiko、Fujimoto Hiroyuki、Nishiyama Hiroyuki、Miyazaki Jun、Kikuchi Eiji、Hinotsu Shiro、Koie Takuya、Ohyama Chikara
  • Journal Title: Japanese Journal of Clinical Oncology Volume: 47 Issue: 7 Pages: 652-659
  • DOI: 10.1093/jjco/hyx051
  • Peer Reviewed / Open Access
• [Journal Article] Development of RNA-FISH Assay for Detection of Oncogenic FGFR3-TACC3 Fusion Genes in FFPE Samples. (2016)
  • Author(s): 7)Kurobe M, Kojima T, Nishimura K, Kandori S, Kawahara T, Yoshino T, Ueno S, Iizumi Y, Mitsuzuka K, Arai Y, Tsuruta H, Habuchi T, Kobayashi T, Matsui Y, Ogawa O, Sugimoto M, Kakehi Y, Nagumo Y, Tsutsumi M, Oikawa T, Kikuchi K, Nishiyama H.
  • Journal Title: PLoS One Volume: 11 Issue: 12 Pages: e0165109-e0165109
  • DOI: 10.1371/journal.pone.0165109
  • NAID: 120005980906
  • Peer Reviewed / Open Access