The exploration of bioactive lipids related to progression of cancer by the next generation mass spectrometry based lipidomics
| Project/Area Number |
26293351
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
神波 大己 京都大学, 医学(系)研究科(研究院), 准教授 (20402836)
寺田 直樹 京都大学, 医学(系)研究科(研究院), 助教 (60636637)
根来 宏光 京都大学, 医学(系)研究科(研究院), 助教 (80708595)
小川 修 京都大学, 医学(系)研究科(研究院), 教授 (90260611)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2014: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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| Keywords | 腎癌 / 前立腺癌 / 脂質 / 質量分析 / リゾホスファチジルコリン / ホスファチジン酸 / 生理活性脂質 / mTOR / PA |
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| Outline of Final Research Achievements |
Prostate tissue samples obtained from radical prostatectomy were analyzed by high-resolution matrix-assisted laser desorption/ionization imaging mass spectrometry (HR-MALDI-IMS). LPC(16:0/OH) was significantly lower expressed in cancer tissue than in benign epithelium. Reduced expression of LPC(16:0/OH) in cancer tissue was an independent predictor of biochemical recurrence after radical prostatectomy.
Analysis of phosphatidic acid (PA) in kidney tissue samples obtained from nephrectomy was performed using Liquid Chromatography Mass Spectrometry (LC/MS). PA(16:0/18:1) was significantly lower expressed in cancer tissue than in benign tissue. PA(16:0/18:1) reduced cell proliferation in the human renal cancer cell line 786-O.
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Report
(4 results)
Research Products
(12 results)
