The exploration of bioactive lipids related to progression of cancer by the next generation mass spectrometry based lipidomics

• Project/Area Number: 26293351
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Kyoto University
• Principal Investigator: Yamasaki Toshinari 京都大学, 医学(系)研究科(研究院), 講師 (00607749)
• Co-Investigator(Kenkyū-buntansha): 神波 大己 京都大学, 医学(系)研究科(研究院), 准教授 (20402836) ; 寺田 直樹 京都大学, 医学(系)研究科(研究院), 助教 (60636637) ; 根来 宏光 京都大学, 医学(系)研究科(研究院), 助教 (80708595) ; 小川 修 京都大学, 医学(系)研究科(研究院), 教授 (90260611)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000); Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2014: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
• Keywords: 腎癌 / 前立腺癌 / 脂質 / 質量分析 / リゾホスファチジルコリン / ホスファチジン酸 / 生理活性脂質 / mTOR / PA

Outline of Final Research Achievements

Prostate tissue samples obtained from radical prostatectomy were analyzed by high-resolution matrix-assisted laser desorption/ionization imaging mass spectrometry (HR-MALDI-IMS). LPC(16:0/OH) was significantly lower expressed in cancer tissue than in benign epithelium. Reduced expression of LPC(16:0/OH) in cancer tissue was an independent predictor of biochemical recurrence after radical prostatectomy.
Analysis of phosphatidic acid (PA) in kidney tissue samples obtained from nephrectomy was performed using Liquid Chromatography Mass Spectrometry (LC/MS). PA(16:0/18:1) was significantly lower expressed in cancer tissue than in benign tissue. PA(16:0/18:1) reduced cell proliferation in the human renal cancer cell line 786-O.

Research Products

• [Journal Article] Decreased expression of lysophosphatidylcholine (16:0/OH) in high resolution imaging mass spectrometry independently predicts biochemical recurrence after surgical treatment for prostate cancer. (2015)
  • Author(s): 後藤崇之
  • Journal Title: Prostate. Volume: 75 Issue: 16 Pages: 1821-1830
  • DOI: 10.1002/pros.23088
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 高解像度質量顕微鏡を用いたリゾホスファチジルコリン(16:0/OH)の発現低下は前立腺癌の術後生化学的再発の独立予測因子である (2016)
  • Author(s): 後藤崇之
  • Organizer: 第104回泌尿器科学会総会
  • Place of Presentation: 仙台
  • Year and Date: 2016-04-23
• [Presentation] 高解像度質量顕微鏡を用いた前立腺癌組織in situ脂質分布解析による新規バイオマーカー探索 (2016)
  • Author(s): 後藤崇之
  • Organizer: 第25回泌尿器科分子・細胞研究会
  • Place of Presentation: 大阪
  • Year and Date: 2016-02-26
• [Presentation] 高解像度質量顕微鏡を用いた前立腺癌組織in situ脂質分布解析による新規バイオマーカー探索 (2016)
  • Author(s): 後藤崇之
  • Organizer: 第32回前立腺シンポジウム
  • Place of Presentation: 品川
• [Presentation] 前立腺がんの新規バイオマーカー探索 (2016)
  • Author(s): 後藤崇之
  • Organizer: 京滋北陸Urological Expert Meeting
  • Place of Presentation: 京都
  • Invited
• [Presentation] 質量顕微鏡を用いた前立腺癌研究の成果 (2016)
  • Author(s): 後藤崇之
  • Organizer: 泌尿器科癌における脂質代謝を考える会
  • Place of Presentation: つくば市
  • Invited
• [Presentation] In situ lipid profiling of prostate cancer tissues using high resolution imaging mass spectrometry (2014)
  • Author(s): 後藤崇之
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 高解像度質量顕微鏡を用いた前立腺癌組織のin situ 脂質プロファイリング (2014)
  • Author(s): 後藤崇之
  • Organizer: 前立腺生物学シンポジウム
  • Place of Presentation: 伊勢志摩
  • Year and Date: 2014-06-26 – 2014-06-27
• [Presentation] 高解像度質量顕微鏡を用いた前立腺癌組織のin situ 脂質プロファイリング (2014)
  • Author(s): 後藤崇之
  • Organizer: 第102回日本泌尿器科学会総会
  • Place of Presentation: 神戸
  • Year and Date: 2014-04-24 – 2014-04-27
• [Presentation] The expression profile of phosphatidylinositol in high resolution imaging mass spectrometry is a potential biomarker for prostate cancer (2014)
  • Author(s): 後藤崇之
  • Organizer: 29th Annual EAU Congress
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 2014-04-11 – 2014-04-15
• [Book] 月刊腎臓内科・泌尿器科 (2016)
  • Author(s): 後藤崇之
  • Total Pages: 112
  • Publisher: 科学評論社
• [Remarks] 京都大学医学研究科泌尿器科学教室HP
  • URL: http://www.urology.kuhp.kyoto-u.ac.jp/