| Project/Area Number |
26293360
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Osaka University |
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Principal Investigator |
Sawada Kenjiro 大阪大学, 医学(系)研究科(研究院), 講師 (00452392)
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| Co-Investigator(Kenkyū-buntansha) |
馬淵 誠士 大阪大学, 医学(系)研究科(研究院), 助教 (00452441)
木村 正 大阪大学, 医学(系)研究科(研究院), 教授 (90240845)
橋本 香映 大阪大学, 医学(系)研究科(研究院), 助教 (90612078)
磯部 晶 大阪大学, 医学(系)研究科(研究院), 助教 (60397619)
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| Research Collaborator |
Kinose Yasuto
Nakamura Koji
Yoshimura Akihiko
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2016: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Keywords | Ovarian cancer / peritoneal dissemination / microRNA / exosome / paclitaxel / 卵巣癌 / マイクロRNA / CD44 miR-99a / バイオマーカー / 腹膜播種 / 卵巣がん / エクソソーム / パクリタキセル耐性 / 低酸素刺激 |
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| Outline of Final Research Achievements |
The overcome of paclitaxel resistance is a critical issue in ovarian cancer treatment. We aimed to identify key miRNAs which regulate paclitaxel resistance and to pursue those potential as therapeutic targets. Two paclitaxel resistant ovarian cancer cell lines were established by a continuous exposure. MiRNA PCR arrays were performed and miR-194 was found to be one of down-regulated miRNAs. We found that miR-194 acts as a tumor suppressor miRNA through the sensitization to paclitaxel and can be considered as a therapeutic target for paclitaxel-resistant ovarian cancer. Further, we aimed to identify the functional roles of ovarian cancer-derived exosomes during peritoneal dissemination. Exosomes were isolated from ovarian cancer cell lines. Ovarian cancer invasion was significantly promoted in the presence of exosome-treated human peritoneal mesothelial cells (HPMCs). We proved that ovarian cancer-derived exosomes transfer CD-44 to HPMCs, which can facilitate ovarian cancer invasion.
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