| Budget Amount *help |
¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000)
Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
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| Outline of Final Research Achievements |
An oocyte aging is strongly related with impairing totipotency of zygote and X-chromosome inactivation of female embryos is partially key molecule feature on acquiring totipotency. We aimed to explore molecular mechanism on X-chromosome inactivation process in preimplantation development using mice model. An our first accomplishment showed that histone 3 lysine 9 tri-methylation (H3K9me3) was involved in Xm (maternal X-chromosome)-Xist derepression from early preimplantation phases. We also found that the chromatin state at the Xist genomic locus became markedly condensed as oocyte growth proceeded. Xm-Xist was derepressed by chromatin alterations, the derepression was stably maintained and rescued paternal Xist mutational lethality, indicating that loss of Xm-Xist imprinting was irreversible. We propose that chromatin condensation and fine-tuning of Rnf12 dosage are crucial for Xist imprint maintenance by silencing Xm-Xist (Fukda, et al. Plos genetics 2016).
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