Development of the self-defense inducing material

• Project/Area Number: 26293403
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Hokkaido University
• Principal Investigator: Sano Hidehiko 北海道大学, 歯学研究科(研究院), 教授 (90205998)
• Co-Investigator(Kenkyū-buntansha): 松田 康裕 北海道医療大学, 歯学部, 講師 (50431317)
• Co-Investigator(Renkei-kenkyūsha): SELIMOVIC Denis 北海道大学, 歯学研究科, 准教授 (20777776)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000); Fiscal Year 2016: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2014: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: ナノテクノロジー / 歯質接着 / 抗菌タンパク / 接着 / 生体適合性

Outline of Final Research Achievements

When using Colloidal Platinum Nanoparticls (CPN) as a primer solution, bonding to caries-affected dentin with Cleafil SE2 did not show improvement in bond strength. Two step dentin conditioner created with the combination of acid and CPN was effective for improving bond strength but affected by the moisture of dentin to be bonded.
When CPN was applied to Hela cells, immunoblot demonstrated the expression of Chromogranin A and its related proteins, showing the level of the expression was in proportion to the concentration of CPN. So far, it is not clear the machanizm of the expression of the antibacterial proteins. Future study is important disclose the mechanizm of the phenomenon.