Regeneration of rat molar pulp tissue by the implantation of stem cells from incisor pulp: development of a rat model of autologous pulp tissue engineering
| Project/Area Number |
26293405
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
OKIJI Takashi 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (80204098)
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| Co-Investigator(Kenkyū-buntansha) |
吉羽 永子 新潟大学, 医歯学総合病院, 講師 (10323974)
吉羽 邦彦 新潟大学, 医歯学系, 准教授 (30220718)
大島 勇人 新潟大学, 医歯学系, 教授 (70251824)
金子 友厚 新潟大学, 医歯学総合病院, 助教 (70345297)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Keywords | 歯学 / 歯内治療学 / 歯髄再生 / 歯髄幹細胞 / 血管内皮細胞 / スキャホールド |
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| Outline of Final Research Achievements |
This study aimed to establish an experimental autologous coronal pulp regeneration model using rat molars and examine whether co-implantation of endothelial cells with stem cells accelerates pulp tissue regeneration. Rat bone marrow mesenchymal stem cells (MSCs) with PLLA/peptide hydrogel constructs were implanted into the coronal pulp chamber of pulpotomized maxillary first molars of Wistar rats. One week after the implantation, a pulp-like tissue was generated in the pulp chamber. In teeth in which rat endothelial cells were co-implanted with MSCs, gene expression levels of pro-angiogenic factors such as Cxcl1 and dentin sialophosphoprotein were elevated compared with MSC-implanted teeth. The co-implanted teeth also showed accelerated scaffold absorption and complete dentin bridge formation. A similar analysis is in progress using stem cells isolated from rat incisor pulp tissues.
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Report
(4 results)
Research Products
(37 results)
