Development of a novel orofacial bone regenerative method depending on the angiogenesis and lymphangiogenesis.
| Project/Area Number |
26293414
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Prosthodontics/ Dental materials science and
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| Research Institution | Kagoshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
小戝 健一郎 鹿児島大学, 医歯学域医学系, 教授 (90258418)
朝比奈 泉 長崎大学, 医歯(薬)学総合研究科, 教授 (30221039)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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| Keywords | 再生医療 / 間葉系幹細胞 / 骨再生 / 骨増生 / 顎骨増生 / 血管再生 / 顎堤増生 |
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| Outline of Final Research Achievements |
In the present study, we succeeded in development of a novel method to construct cell/scaffold complex using the sodium alginate. The uniformity and operativity of the cell/scaffold complex was apparently improved by using this novel method. Next, we constructed the mesenchymal stem cells (MSCs)/scaffold complex using sodium alginate, and then transplanted them on the mice calvariae. At 4 weeks after surgery, newly formed bone was confirmed in the grafts. Furthermore, neovessel and neolymphvessel formation was induced in the grafts area. VEGF-C is known to stimulate lymphangiogenesis, so, we next investigated whether VEGF-C modulates osteogenic differentiation of MSCs. Treatment with VEGF-C significantly induced the osteogenic differentiation of MSCs. In addition, we also revealed that treatment with VEGF-C significantly enhanced MSCs migration. These results indicate that not only angiogenesis but lymphangiogenesis may play an important role in bone formation process.
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] Regulation and Biological Significance of Formation of Osteoclasts and Foreign Body Giant Cells in an Extraskeletal Implantation Model2016
Author(s)
Ahmed GJ, Tatsukawa E, Morishita K, Shibata Y, Suehiro F, Kamitakahara M, Yokoi T, Koji T, Umeda M, Nishimura M, Ikeda T
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Journal Title
ACTA HISTOCHEMICA ET CYTOCHEMICA
Volume: 49
Issue: 3
Pages: 97-107
DOI
NAID
ISSN
0044-5991, 1347-5800
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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