Trans-differentiation of human fibroblasts into chondrocytes and osteocytes and its application for regenerative medicine
| Project/Area Number |
26293419
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
IKEDA Masa-Aki 東京医科歯科大学, 医歯(薬)学総合研究科, 准教授 (20193211)
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| Co-Investigator(Kenkyū-buntansha) |
池田 やよい 愛知学院大学, 歯学部, 教授 (00202903)
大谷 清 関西学院大学, 理工学部, 教授 (30201974)
春日井 昇平 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (70161049)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2016: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2015: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Keywords | 骨 / 軟骨 / 繊維芽細胞 / 間葉系幹細胞 / 分化転換 / 細胞培養 / 増殖因子 / 再生医療 / 線維芽細胞 / 培養 / リプログラミング / 再生医学 / 幹細胞 |
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| Outline of Final Research Achievements |
The objectives of this study were to determine (1) culture conditions that enable human fibroblasts to trans-differentiate into chondrocytes and osteocytes, and (2) culture conditions that enable mesenchymal stem cells (MSCs) to maintain proliferation and multilineage differentiation potential in vitro long-term culture. We found a combination of culture supplements that enabled to trans-differentiate human dermal fibroblasts into osteocytes and adipocytes, but not chondrocytes. Furthermore, we found a combination of culture supplements that enabled to maintain proliferation potential of bone marrow-derived MSCs (BM-MSCs) in vitro. The long-term cultured MSCs kept osteogenic, adipogenic, and chondrogenic differentiation capacity and expressed the lineage-specific differentiation markers. These results indicate that the extended culture conditions enabled to maintain proliferation capacity of BM-MSCs while retaining their multipotent differentiation capacity.
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Report
(4 results)
Research Products
(21 results)
