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Trans-differentiation of human fibroblasts into chondrocytes and osteocytes and its application for regenerative medicine

Research Project

Project/Area Number 26293419
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Dental engineering/Regenerative dentistry
Research InstitutionTokyo Medical and Dental University

Principal Investigator

IKEDA Masa-Aki  東京医科歯科大学, 医歯(薬)学総合研究科, 准教授 (20193211)

Co-Investigator(Kenkyū-buntansha) 池田 やよい  愛知学院大学, 歯学部, 教授 (00202903)
大谷 清  関西学院大学, 理工学部, 教授 (30201974)
春日井 昇平  東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (70161049)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2016: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2015: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Keywords骨 / 軟骨 / 繊維芽細胞 / 間葉系幹細胞 / 分化転換 / 細胞培養 / 増殖因子 / 再生医療 / 線維芽細胞 / 培養 / リプログラミング / 再生医学 / 幹細胞
Outline of Final Research Achievements

The objectives of this study were to determine (1) culture conditions that enable human fibroblasts to trans-differentiate into chondrocytes and osteocytes, and (2) culture conditions that enable mesenchymal stem cells (MSCs) to maintain proliferation and multilineage differentiation potential in vitro long-term culture. We found a combination of culture supplements that enabled to trans-differentiate human dermal fibroblasts into osteocytes and adipocytes, but not chondrocytes. Furthermore, we found a combination of culture supplements that enabled to maintain proliferation potential of bone marrow-derived MSCs (BM-MSCs) in vitro. The long-term cultured MSCs kept osteogenic, adipogenic, and chondrogenic differentiation capacity and expressed the lineage-specific differentiation markers. These results indicate that the extended culture conditions enabled to maintain proliferation capacity of BM-MSCs while retaining their multipotent differentiation capacity.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (21 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (2 results) Journal Article (8 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 8 results,  Acknowledgement Compliant: 2 results,  Open Access: 3 results) Presentation (11 results) (of which Int'l Joint Research: 4 results,  Invited: 2 results)

  • [Int'l Joint Research] University of Naples Federico II/The University of Campania(Italy)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research]

    • Related Report
      2016 Annual Research Report
  • [Journal Article] FUCA1 is induced by wild-type p53 and expressed at different levels in thyroid cancers depending on p53 status.2017

    • Author(s)
      Tsuchida N, Ikeda MA, Grieco M, Vecchio G
    • Journal Title

      International Journal of Oncology

      Volume: 51 Issue: 6 Pages: 2043-204

    • DOI

      10.3892/ijo.2017.3968

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Extended Culture Conditions for Multipotent Bone Marrow-Derived Mesenchymal Stem Cells2016

    • Author(s)
      Zhang K, Ikeda Y, Kasugai S, Ikeda MA
    • Journal Title

      Journal of the Japanese Stomatological Society

      Volume: 83 Pages: 13-23

    • NAID

      40020827576

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Critical role of ARID3B in the expression of pro-apoptotic p53-target genes and apoptosis2015

    • Author(s)
      Pratama E, Tian X, Lestari W, Iseki S, Ichwan S J A, Ikeda MA
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 468 Issue: 1-2 Pages: 248-254

    • DOI

      10.1016/j.bbrc.2015.10.121

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Cyclin E marks quiescent neural stem cells and caspase-3-positive newborn cells during adult hippocampal neurogenesis in mice2015

    • Author(s)
      Ikeda Y, Ikeda MA
    • Journal Title

      Neurosci Lett.

      Volume: 607 Pages: 90-96

    • DOI

      10.1016/j.neulet.2015.09.017

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Effect of X-Irradiation at Different Stages in the Cell Cycle on Individual Cell–Based Kinetics in an Asynchronous Cell Population2015

    • Author(s)
      Tsuchida E, Kaida A, Pratama E, Ikeda MA, Suzuki K, Harada K, Miura M
    • Journal Title

      PLoS One

      Volume: 10 Issue: 6 Pages: e0128090-e0128090

    • DOI

      10.1371/journal.pone.0128090

    • NAID

      120006986960

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Chitosan exerts anticancer activity through induction of apoptosis and cell cycle arrest in oral cancer cells2014

    • Author(s)
      3.Yuniardini S Wimardhani, Dewi F Suniarti, Hans J Freisleben, Septelia I Wanandi, Nurjati C Siregar, Masa-Aki Ikeda
    • Journal Title

      Journal of Oral Science

      Volume: 56 Issue: 2 Pages: 119-126

    • DOI

      10.2334/josnusd.56.119

    • NAID

      130004479925

    • ISSN
      1343-4934, 1880-4926
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Apoptotic Activities of Thymoquinone, an Active Ingredient of Black Seed (Nigella sativa), in Cervical Cancer Cell Lines2014

    • Author(s)
      2.Ichwan SJ, Al-Ani IM, Bilal HG, Suriyah WH, Taher M, Ikeda MA
    • Journal Title

      Chin. J Physiol.

      Volume: 57 Issue: 5 Pages: 249-255

    • DOI

      10.4077/cjp.2014.bab190

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of p27Kip1 and E-cadherin in Head and Neck Squamous Cell Carcinoma of Indonesian Patients.2014

    • Author(s)
      1.Auerkari EI, Joewono V, Handjari DR, Sarwono1 AT, Suhartono AW, Eto K, Ikeda MA
    • Journal Title

      Open Dentistry J

      Volume: 8 Issue: 1 Pages: 136-143

    • DOI

      10.2174/1874210601408010136

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Critical role of ARID3B in the expression of E2F-responsive genes and cell proliferation2016

    • Author(s)
      Saadat KASM,Pratama E, Lestari W,Ma T, Ohtani K,Ikeda MA
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] Opposite roles of lncRNA ERICD (E2F1-regulated inhibitor of cell death) and ARID3A (AT-rich interaction domain 3A) inosteosarcoma, glioblastoma and lung cancer2016

    • Author(s)
      Arman K, Igci YZ, Saadat KASM, Altan Z, Sahin Y, Ikeda MA, Igci M
    • Organizer
      The 41th FEBS Congress
    • Place of Presentation
      Ephesus(Turkey)
    • Year and Date
      2016-09-03
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Novel Candidates of Therapeutic Targets for Head and Neck Squamous Cell Carcinomas Defined by Recent Comprehensive Mutation Analysis2016

    • Author(s)
      Ikeda, MA
    • Organizer
      The 17th Scientific and Refresher Course in Dentistry (KPPIKG 2016)
    • Place of Presentation
      Jakarta, Indonesia
    • Year and Date
      2016-02-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ARID3B promotes gene expression critical for E2f-mediated cell cycle progression and p53-mediated apoptosis2015

    • Author(s)
      Pratama E, Saadat KASM,Lestari W,Ichwan S, Iseki S, Ohtani K,Ikeda MA
    • Organizer
      第38回日本分子生物学会年会・第88回日本生化学会大会合同大会
    • Place of Presentation
      神戸国際会議場、兵庫県、神戸市
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] Expression study of E2F-target genes in osteosarcoma (U2OS) and glioblastoma (T98G) cell lines after knocking down ARID3A and ARID3B2015

    • Author(s)
      Saadat KASM, Kaifee A, Ahmet A, Iseki S, Ohtani K, Ikeda MA
    • Organizer
      XIV. National Congress of Medical Biology and Genetics
    • Place of Presentation
      Oludeniz-Fethiye, Turkey
    • Year and Date
      2015-10-27
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Overexpression of ARID3 facilitates E2F-dependent apoptosis2015

    • Author(s)
      Saadat KASM, Kaifee A, Ahmet A, Ohtani K, Ikeda MA
    • Organizer
      XIV. National Congress of Medical Biology and Genetics
    • Place of Presentation
      Oludeniz-Fethiye, Turkey
    • Year and Date
      2015-10-27
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Maintenance of Stemness in Mesenchymal Stem Cells during Long-Term Culture2014

    • Author(s)
      Zhang Kui, Shohei Kasugai, Masa-Aki Ikeda
    • Organizer
      第79回口腔病学会学術大会
    • Place of Presentation
      東京
    • Year and Date
      2014-12-05 – 2014-12-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] Critical roles for ARID3B in Expression of Proapoptotic p53-Target Genes and Cell Death Following DNA Damage2014

    • Author(s)
      Endrawan Pratama, Sachiko Iseki, Masa-Aki Ikeda
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Immunohistochemical detection of cyclin E in non-proliferating neurons of the mouse adult hippocampal dentate gyrus2014

    • Author(s)
      Ikeda Y, Ikeda MA
    • Organizer
      Society for Neuroscience 2014 Annual Meeting
    • Place of Presentation
      Washington DC, USA
    • Year and Date
      2014-11-15 – 2014-11-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] Mesenchymal Stem Cells and Bone Tissue Engineering - Overview and Our Approach –2014

    • Author(s)
      Ikeda, MA
    • Organizer
      Regional Oral Biology Scientific Meeting 2014
    • Place of Presentation
      Depok, Indonesia
    • Year and Date
      2014-10-30 – 2014-10-31
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] アポトーシス関連p53標的遺伝子の発現と細胞死誘導におけるDNA結合因子ARID3Bの重要な役割2014

    • Author(s)
      Endrawan Pratama、井関祥子、池田正明
    • Organizer
      第56回歯科基礎医学会学術大会・総会
    • Place of Presentation
      福岡
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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