Development of optical hypoxic environment for manufacturing a tissue-engineered oral mucosa by utilizing a hypoxia-sensing luminescent probe
Project/Area Number |
26293420
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Dental engineering/Regenerative dentistry
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Research Institution | Niigata University |
Principal Investigator |
IZUMI KENJI 新潟大学, 医歯学系, 教授 (80242436)
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Co-Investigator(Kenkyū-buntansha) |
飛田 成史 群馬大学, 大学院理工学府, 教授 (30164007)
前田 健康 新潟大学, 医歯学系, 教授 (40183941)
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Project Period (FY) |
2014-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2016: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
|
Keywords | 低酸素 / 再生医療 / 口腔粘膜 / 角化細胞 / 幹細胞ニッチ / 口腔粘膜角化細胞 / 発光プローブ / 口腔粘膜上皮細胞 / 上皮細胞 / プローブ |
Outline of Final Research Achievements |
It is necessary to calibrate cellular oxygen levels to quantify oxygen levels of an epithelial layer within a tissue-engineered oral mucosa for clinical application. In this study, we used a new iridium complex BTPDM1 as an oxygen sensing probe, at a concentration of 500nM. When culturing oral keratinocytes in a monolayer, in a variety of culture conditions such as cell plating density and calcium concentration, we measured intracellular oxygen levels using confocal phosphorescence lifetime imaging microscope. Our results showed cellular oxygen levels appeared to be dependent on the size of cells, and the development of cornified layer did not cause severe hypoxic condition in the underlying cell layer. This suggests the intraepithelial oxygen levels of the EVPOME grafts may depend on the cellular differentiation and the quality of cornified layer.
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Report
(5 results)
Research Products
(11 results)
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[Presentation] Hypoxia induces undifferentiated phenotype of oral keratinocytes in vitro2014
Author(s)
Kato H, Izumi K, Shiomi A, Uenoyama A, Kuo S, Feinberg SE, Maeda T
Organizer
TERMIS Americas 2014
Place of Presentation
Renaissance Hotel, Washington, DC Downtown, Washington DC, USA
Year and Date
2014-12-13 – 2014-12-16
Related Report
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