role of preodontoblast in tooth development

• Project/Area Number: 26293435
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: The University of Tokushima
• Principal Investigator: IWAMOTO Tsutomu 徳島大学, 大学院医歯薬学研究部, 教授 (90346916)
• Co-Investigator(Kenkyū-buntansha): 長谷川 智一 徳島大学, 大学院医歯薬学研究部, 講師 (50274668)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000); Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2015: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2014: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
• Keywords: 前象牙芽細胞 / Pannexin / Panx3 / ATP channel / AMPK / 象牙芽細胞 / 細胞増殖 / 細胞分化 / 歯の発生 / 細胞間結合 / パネキシン / ギャップ結合分子

Outline of Final Research Achievements

The purpose of this study is to clarify the roles of preodontoblasts in tooth development. Here we have found that the expression of Panx3 was predominately localized in preo- dontoblasts that arise from dental papilla cells and can differentiate into dentin-secreting odontoblasts. Overexpression of Panx3 in dental mesenchymal cells reduced cell proliferation via up-regulation of p21, and promoted the BMP2-induced phosphorylation of Smad1/5/8 and the expression of Dspp, a marker of differentiated odontoblasts. Furthermore, Panx3 functions ATP release into the extracellular space through its hemichannel and induced the phosphorylation of AMPK. Our results suggest that Panx3 modulates intracellular ATP levels, resulting in the inhibition of odontoblast proliferation through the AMPK/p21 signaling pathway and promotion of cell differentiation by the BMP/Smad signaling pathway.

Research Products

• [Journal Article] Recruitment of mesenchymal stem cells by stromal cell-derived factor 1α in pulp cells from deciduous teeth (2015)
  • Author(s): Akazawa Y., Hasegawa T., Yoshimura Y., Chosa N., Asakawa T., Ueda K., Sugimoto A., Kitamura T., Nakagawa H., Ishisaki A., Iwamoto T.
  • Journal Title: International Journal of Molecular Medicine Volume: 36 Issue: 2 Pages: 442-448
  • DOI: 10.3892/ijmm.2015.2247
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Interaction between Fibronectin and β1 Integrin is Essential for Tooth Development. (2015)
  • Author(s): Saito, K., Fukumoto, E., Yamada, A., Yuasa, K., Yoshizaki, K., Iwamoto, T., Saito, M., Nakamura, T., and Fukumoto, S.
  • Journal Title: PLOS one Volume: 10(4) Issue: 4 Pages: 1-12
  • DOI: 10.1371/journal.pone.0121667
  • NAID: 120006872347
  • Peer Reviewed / Open Access
• [Journal Article] Dental findings and management in a child with hypomelanosis of Ito. (2014)
  • Author(s): Tomokazu Hasegawa, Yuki Akazawa, Takamasa Kitamura, Asuna Sugimoto, Kimiko Ueda, Tsutomu Iwamoto
  • Journal Title: Pediatric Dental Journal Volume: 24 Issue: 3 Pages: 173-177
  • DOI: 10.1016/j.pdj.2014.08.002
  • Peer Reviewed / Open Access
• [Presentation] 間葉系幹細胞の分化運命決定に関与 する圧受容体PIEZO1 (2016)
  • Author(s): 杉本明日菜，岩本 勉
  • Organizer: 第17回先端歯学スクール
  • Place of Presentation: WITH THE STYLE FUKUOKA（福岡県福岡市）
  • Year and Date: 2016-09-29
• [Presentation] Hydrostatic pressure regulates cell fate decision in human mesenchymal stem cells (2016)
  • Author(s): Sugimoto Asuna, Masayuki Shono, Tsutomu Iwamoto
  • Organizer: 10th Biennial Conference of the Pediatric Dentistry Association of Asia
  • Place of Presentation: 東京ドームホテル（東京都文京区）
  • Year and Date: 2016-05-26
  • Int'l Joint Research
• [Presentation] 象牙質修復に関わる間葉系幹細胞の乳歯歯髄細胞由来SDF-1αによる制御 (2015)
  • Author(s): 赤澤 友基, 長谷川 智一, 岩本 勉
  • Organizer: 第53回日本小児歯科学会大会
  • Place of Presentation: 広島国際会議場（広島県広島市）
  • Year and Date: 2015-05-21
• [Presentation] 乳歯歯根膜由来細胞のSDF-1αを介した歯周組織恒常性に関わる細胞間相互作用 (2015)
  • Author(s): 長谷川 智一, 赤澤 友基, 吉村 善隆, 帖佐 直幸, 浅川 剛吉, 杉本 明日菜, 北村 尚正, 上田(山口) 公子, 中川 弘, 白石 真紀, 石崎 明, 岩本 勉
  • Organizer: 第53回日本小児歯科学会大会
  • Place of Presentation: 広島国際会議場（広島県広島市）
  • Year and Date: 2015-05-21