Establishment of ECM related Periodontal medicine

• Project/Area Number: 26293437
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Periodontology
• Research Institution: Osaka University
• Principal Investigator: Yamada Satoru 大阪大学, 歯学部附属病院, 講師 (40359849)
• Co-Investigator(Kenkyū-buntansha): 森崎 隆幸 国立研究開発法人国立循環器病研究センター, その他部局等, その他 (30174410) ; 竹立 匡秀 大阪大学, 歯学研究科(研究院), 助教 (60452447) ; 村上 伸也 大阪大学, 歯学研究科(研究院), 教授 (70239490)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000); Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
• Keywords: 歯周医学 / 遺伝子改変マウス / 細胞外マトリックス / ECM / 細胞外基質 / 疾患モデル

Outline of Final Research Achievements

In this study, we investigated common molecules which are expressed and function in periodontal tissue and other organs/tissues to understand molecular pathogenesis of periodontal medicine. It was shown that PLAP-1 which is expressed in periodontal ligament and adipose tissue functions as positive regulator for adipogenesis. PLAP-1 KO mice shows the improvement of HFD-induced overweight and significantly high glucose tolerance and enhanced insulin sensitivity compared to WT mice. HFD-induced alveolar bone resorption in PLAP-1 KO mice were also less. In LDS model mouse, in which TGF-β signal is disordered alveolar bone loss by P.g inoculation was significantly increased, compared to WT. The LDS macrophages showed an up-regulated response to LPS compared to that of WT. These results suggest that PLAP-1 plays important roles in HFD-induced obesity and periodontitis and the abnormality of TGF-β signal may accelerate pathogenesis or progress of periodontitis.

Research Products

• [Journal Article] Transcriptome Reveals Cathepsin K in Periodontal Ligament Differentiation. (2016)
  • Author(s): Yamada S, Ozaki N, Tsushima K, Yamaba S, Fujihara C, Awata T, Sakashita H, Kajikawa T, Kitagaki J, Yamashita M, Yanagita M, Murakami S
  • Journal Title: J Dent Res Volume: 95 Issue: 9 Pages: 1026-33
  • DOI: 10.1177/0022034516645796
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Transcriptome reveals cathepsin K in periodontal ligament differentiation (2016)
  • Author(s): S. Yamada, N. Ozaki, K. Tsushima, S. Yamaba, C. Fujihara, T. Awata, H. Sakashita, T. Kajikawa, J. Kitagaki, M. Yamashita, M. Yanagita, S. Murakami
  • Journal Title: Journal of Dental Research Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] PLAP-1/aspirin regulates inflammation through Toll-like receptors 2 and 4 (2015)
  • Author(s): S Yamaba, S Yamada, T Kajikawa, T Awata, H Sakashita, K Tsushima, C Fujihara, M Yanagita, S Murakami
  • Journal Title: J Dent Res Volume: 94 Issue: 12 Pages: 1706-14
  • DOI: 10.1177/0022034515606859
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] PLAP-1/asporin positively regulates FGF-2 activity (2015)
  • Author(s): T Awata, S Yamada, K Tsushima, H Sakashita, S Yamaba, T Kajikawa, M Yamashita, M Takedachi, M Yanagita, M Kitamura, S Murakami.
  • Journal Title: J Dent Res Volume: 94 Issue: 10 Pages: 1417-24
  • DOI: 10.1177/0022034515598507
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Characterization of a novel periodontal ligament-specific periostin isoform (2014)
  • Author(s): S Yamada, T Tauchi, T Awata, K Maeda, T Kajikawa, M Yanagita, S Murakami
  • Journal Title: J Dent Res Volume: 93 Issue: 9 Pages: 89-95
  • DOI: 10.1177/0022034514543015
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Analysis of pathomolecular condition of periodontitis with Loeys-Dietz Syndrome (2017)
  • Author(s): Tsushima K, Yamada S, Awata T, Yamaba S, Sakashita H, Kinoshita M, Takedachi M, Kitagaki J, Yamashita M, Yanagita M, Nozaki T, Saito M, Murakami S
  • Organizer: 2017 IADR GENERAL SESSION
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2017-03-22
  • Int'l Joint Research
• [Presentation] 脂肪分化による脂肪分化調節機構の解明 (2016)
  • Author(s): 阪下裕美、山田聡、津島賢一朗、木下昌毅、藤原千春、梶川哲宏、粟田敏仁、山羽聡子、村上伸也
  • Organizer: 第59回秋季日本歯周病学会学術大会
  • Place of Presentation: 朱鷺メッセ（新潟県新潟市）
  • Year and Date: 2016-10-07
• [Presentation] Loeys-Dietz症候群モデルマウスを用いた歯周病の分子病態解析 (2016)
  • Author(s): 津島賢一朗、山田聡、粟田敏仁、山羽聡子、阪下裕美、木下昌毅、竹立匡秀、北垣次郎太、山下元三、柳田学、野崎剛徳、北村正博、齋藤正寛、村上伸也
  • Organizer: 第59回秋季日本歯周病学会学術大会
  • Place of Presentation: 朱鷺メッセ（新潟県新潟市）
  • Year and Date: 2016-10-07
• [Presentation] Pathomolecular relationship between Loeys-Dietz syndrome and aggressive periodontitis (2015)
  • Author(s): K. Tsushima, S. Yamada, T. Awata, S. Yamaba, H. Sakashita, M Takedachi, J. Kitagaki, M. Yamashita, M. Yanagita, T. Nozaki, M. Kitamura, M. Saito, S. Murakami
  • Organizer: 63rd annual meeting of Japanese association for dental research
  • Place of Presentation: 福岡国際会議場（福岡県・福岡市）
  • Year and Date: 2015-10-30
  • Int'l Joint Research
• [Presentation] 脂肪分化過程におけるPLAP-1の機能解析 (2015)
  • Author(s): 阪下裕美、山田 聡、津島賢一朗、山羽聡子、粟田敏仁、梶川哲宏、竹立匡秀、村上伸也
  • Organizer: 第58回春季日本歯周病学会学術大会
  • Place of Presentation: 幕張メッセ（千葉県・千葉市）
  • Year and Date: 2015-05-15
• [Presentation] Loeys-Dietz症候群モデルマウスの作製と解析 (2015)
  • Author(s): 津島賢一朗、山田 聡、粟田敏仁、山羽聡子、阪下裕美、竹立匡秀、北垣次郎太、山下元三、柳田 学、野崎剛徳、北村正博、齋藤正寛、村上伸也
  • Organizer: 第58回春季日本歯周病学会学術大会
  • Place of Presentation: 幕張メッセ（千葉県・千葉市）
  • Year and Date: 2015-05-15
• [Presentation] 脂肪細胞分化過程におけるPLAP-1の機能解析 (2015)
  • Author(s): 阪下裕美，山田 聡，津島賢一朗，山羽聡子，粟田敏仁，梶川哲宏，竹立匡秀，村上伸也
  • Organizer: 第58回日本歯周病学会春季学術大会
  • Place of Presentation: 千葉
  • Year and Date: 2015-05-15
• [Presentation] Loeys-Dietz症候群モデルマウスの作製と解析 (2015)
  • Author(s): 津島賢一朗、山田聡、粟田敏仁、山羽聡子、阪下裕美、竹立匡秀、北垣次郎太、山下元三、柳田学、野崎剛徳、北村正博、斎藤正寛、村上伸也
  • Organizer: 第58回日本歯周病学会春季学術大会
  • Place of Presentation: 千葉
  • Year and Date: 2015-05-15
• [Presentation] Molecular basis of periodontal ligament homeostasis regulatedby PLAP-1/asporin. (2014)
  • Author(s): Satoru Yamada
  • Organizer: International Association of Dental Research
  • Place of Presentation: Cape Town, South Africa
  • Year and Date: 2014-06-27
• [Presentation] 肥満病態におけるPLAP-1の機能解析 (2014)
  • Author(s): 阪下裕美，山田 聡，梶川哲宏，粟田敏仁，山羽聡子，津島賢一朗，竹立匡秀，村上伸也
  • Organizer: 第57回日本歯周病学会春季学術大会
  • Place of Presentation: 岐阜
  • Year and Date: 2014-05-23
• [Presentation] TLRを介したPLAP-1の炎症制御機構 (2014)
  • Author(s): 山羽聡子，山田 聡，粟田敏仁，阪下裕美，津島賢一朗，梶川哲宏，竹立匡秀，柳田 学，村上伸也
  • Organizer: 第57回日本歯周病学会春季学術大会
  • Place of Presentation: 岐阜
  • Year and Date: 2014-05-23