| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2017: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
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| Outline of Final Research Achievements |
In this study, we collected the malaria patient bloods in Thai-Myanmar and established 255 of the cultivable parasite strain. Next, we established the detection method for artemisinin resistance. In this method, we cultured the parasites in the presence of 5 nM of artemisinin for 96 hours and evaluated their resistance in terms of their survivals. By using this method, we identified 7 artemisinin resistant parasites from those obtained cultivable parasites. In contrast to the Cambodian resistant parasite, one of them did not show the resistance at the ring stage. This result indicated that Thai-Myanmar resistant parasite was different from the Cambodian resistant parasites. Furthermore, we identified the gene responsible for redox system as drug resistance gene. This result suggested that the change of the redox condition in the cell may involved in the artemisinin resistance.
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